Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IGJ protein (Right) or empty vector (Left) detected with IGJ antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of IGJ protein in paraffin embedded Carcinoma of Human kidney tissue using IGJ antibody)

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IGJ, Monoclonal Antibody (Cat# AAA108239)

Full Name

IGJ antibody

Gene Names

JCHAIN; IGJ; JCH; IGCJ

Applications

Western Blot, Immunohistochemistry

Purity

IGJ antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKCE protein using PRKCE antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKCE protein (Right) or empty vector (Left) detected with PRKCE antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PRKCE protein (red) or empty vector (blue) stained using PRKCE antibody)

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PRKCE, Monoclonal Antibody (Cat# AAA108069)

Full Name

PRKCE antibody

Gene Names

PRKCE; PKCE; nPKC-epsilon

Applications

Western Blot, Immunofluorescence

Purity

PRKCE antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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WB (Western Blot) (human plasma tissue were subjected to SDS PAGE followed by western blot with AAA102694 (APOA1 Antibody) at dilution of 1:2000)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using AAA102694 (APOA1 Antibody) at dilution of 1:400 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)

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APOA1, Monoclonal Antibody (Cat# AAA102694)

Full Name

APOA1 Mouse Monoclonal

Gene Names

APOA1; apo(a)

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

> 95% as determined by SDS-PAGE
Protein A+G purification

Pricing

$355/0.1 mg $685/2x0.1 mg $995/3x0.1 mg $1,295/4x0.1 mg $1,380/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TMOD1 protein using TMOD1 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TMOD1 protein (Right) or empty vector (Left) detected with TMOD1 antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant TMOD1 protein (red) or empty vector (blue) stained using TMOD1 antibody)

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TMOD1, Monoclonal Antibody (Cat# AAA107374)

Full Name

TMOD1 antibody

Gene Names

TMOD1; TMOD; ETMOD; D9S57E

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

TMOD1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PSMD2 protein (Right) or empty vector (Left) detected with PSMD2 antibody)

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PSMD2 protein using PSMD2 antibody)

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PSMD2, Monoclonal Antibody (Cat# AAA107157)

Full Name

PSMD2 antibody

Gene Names

PSMD2; S2; P97; RPN1; TRAP2

Applications

Western Blot, Immunofluorescence

Purity

PSMD2 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant HP protein using HP antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HP protein (Right) or empty vector (Left) detected with HP antibody)

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HP, Monoclonal Antibody (Cat# AAA106487)

Full Name

HP antibody

Gene Names

HP; BP; HPA1S; HP2ALPHA2

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

HP antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of SLC2A6 protein in paraffin embedded Human endometrium tissue using SLC2A6 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SLC2A6 protein (Right) or empty vector (Left) detected with SLC2A6 antibody)

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SLC2A6 protein using SLC2A6 antibody)

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SLC2A6, Monoclonal Antibody (Cat# AAA106938)

Full Name

SLC2A6 antibody

Gene Names

Slc2a6; Glut6; Glut9; A330096C23; F630103L12Rik

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

SLC2A6 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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WB (Western Blot) (Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate (AAA47045).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTB)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart, using beta Actin Antibody(AAA47045)ACTB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTB Antibody (AAA47045)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells, using beta Actin Antibody)

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beta Actin, Monoclonal Antibody (Cat# AAA47045)

Full Name

Anti-beta Actin Rabbit Monoclonal Antibody

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of NUP98 expression in HeLa cell lysate (AAA47047).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP98 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUP98)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human testis, using NUP98 Antibody(AAA47047)NUP98 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NUP98 Antibody (AAA47047)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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NUP98, Monoclonal Antibody (Cat# AAA47047)

Full Name

Anti-NUP98 Rabbit Monoclonal Antibody

Gene Names

NUP98; ADIR2; NUP96; NUP196

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate (AAA47074).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYLK)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(AAA47074)MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA47074)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Myosin light chain kinase, Monoclonal Antibody (Cat# AAA47074)

Full Name

Anti-Myosin light chain kinase Rabbit Monoclonal Antibody

Gene Names

MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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IF (Immunofluorescence) (ICC/IF analysis of ACTA2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: SW480 cell lysate Lane 3.: HeLa cell lysate)

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ACTA2, Monoclonal Antibody (Cat# AAA48166)

Full Name

ACTA2 antibody

Gene Names

ACTA2; ACTSA

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BECN1 protein using BECN1 antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of BECN1 protein in paraffin embedded Human pancreas tissue using BECN1 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BECN1 protein (Right) or empty vector (Left) detected with BECN1 antibody)

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BECN1, Monoclonal Antibody (Cat# AAA106341)

Full Name

BECN1 antibody

Gene Names

BECN1; ATG6; VPS30; beclin1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

BECN1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of ACY1 protein in paraffin embedded Human liver tissue using ACY1 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACY1 protein (Right) or empty vector (Left) detected with ACY1 antibody)

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ACY1, Monoclonal Antibody (Cat# AAA106346)

Full Name

ACY1 antibody

Gene Names

ACY1; ACY-1; ACY1D; HEL-S-5

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

ACY1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BEST3 protein using BEST3 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BEST3 protein (Right) or empty vector (Left) detected with BEST3 antibody)

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BEST3, Monoclonal Antibody (Cat# AAA106230)

Full Name

BEST3 antibody

Gene Names

BEST3; VMD2L3

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

BEST3 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BECN1 protein using BECN1 antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of BECN1 protein in paraffin embedded Human liver tissue using BECN1 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BECN1 protein (Right) or empty vector (Left) detected with BECN1 antibody)

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BECN1, Monoclonal Antibody (Cat# AAA106258)

Full Name

BECN1 antibody

Gene Names

BECN1; ATG6; VPS30; beclin1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

BECN1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer using SGK1 antibody.)

WB (Western Blot) (Western blot analysis of SGK1 expression in A431 cell lysate.)

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SGK1/Sgk, Monoclonal Antibody (Cat# AAA46976)

Full Name

Anti-SGK1/Sgk Rabbit Monoclonal Antibody

Gene Names

SGK1; SGK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of M6PR expression in Jurkat cell lysate (AAA47006).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGF2R)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using M6PR Antibody(AAA47006)IGF2R was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGF2R Antibody (AAA47006)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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M6PR, Monoclonal Antibody (Cat# AAA47006)

Full Name

Anti-M6PR Rabbit Monoclonal Antibody

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 10H7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 10H7. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

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Alpha Synuclein, Monoclonal Antibody (Cat# AAA104138)

Full Name

Alpha Synuclein Antibody, Clone 10H7

Gene Names

Snca; NACP; alphaSYN; alpha-Syn

Reactivity

Human; Mouse; Rat

Applications

Immunofluorescence, Immunocytochemistry, Dot Blot, Western Blot

Purity

Protein G Purified

Pricing

$525/0.1 mg $1,015/2x0.1 mg $1,485/3x0.1 mg $1,930/4x0.1 mg $2,060/5x0.1 mg

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ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.)

WB (Western Blot) (Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

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Alpha Synuclein, Monoclonal Antibody (Cat# AAA104161)

Full Name

Alpha Synuclein Antibody, Clone 4F1: FITC

Gene Names

Snca; NACP; alphaSYN; alpha-Syn

Reactivity

Human; Mouse; Rat

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Dot Blot, Western Blot

Purity

Protein G Purified

Pricing

$580/0.1 mg $1,120/2x0.1 mg $1,635/3x0.1 mg $2,125/4x0.1 mg $2,290/5x0.1 mg

IHC (Immunohistochemistry) (HeLa cell cultures were stained with AAA76607 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells. Cells were counterstained with our chicken polyclonal antibody to vimentin in red. Blue is a DNA stain.)

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Aurora A/B, Monoclonal Antibody (Cat# AAA76607)

Full Name

Aurora A/B Kinase

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47

Reactivity

Rat, Cow, Pig, Horse, Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry

Purity

Affinity Purified

Pricing

$545/0.1 mL $1,050/2x0.1 mL $1,530/3x0.1 mL $1,985/4x0.1 mL $2,240/5x0.1 mL

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WB (Western Blot) (Western blot analysis of different tissue lysates using mouse mAb to alpha-internexin dilution 1:10,000 in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord.)

IF (Immunofluorescence) (Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to alpha-internexin, MO22102, dilution 1:5,000 in green, and costained with chicken pAb to calretinin 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies.)

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alpha-Internexin/NF 66, Monoclonal Antibody (Cat# AAA76626)

Full Name

alpha-Internexin/NF 66

Gene Names

INA; NEF5; NF-66; TXBP-1

Reactivity

Human, Mouse, Rat, Cat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,105/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Fig-3: Intra cellular flow analysis of IkB alpha in Jurkat using 0.5 ug/10^6 cells of antibody (Clone: ABM10F4). Green represents isotype control; red represents anti-IkB alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

FCM/FACS (Flow Cytometry) (Fig-2: Intra cellular flow analysis of IkB alpha in Hela using 0.5 ug/10^6 cells of antibody (Clone: ABM10F4). Green represents isotype control; red represents anti-IkB alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

WB (Western Blot) (Fig-1: Western blot analysis of IkB alpha. Anti-IkB alpha antibody (Clone: ABM10F4) was used at 4 ug/ml on MCF7 and PC3 lysate.)

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IkB alpha, Monoclonal Antibody (Cat# AAA78273)

Full Name

Monoclonal Antibody to IkB alpha (Clone: ABM10F4)

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Purified
Protein G Chromatography

Pricing

$215/0.025 mg $395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Fig-2: Intra cellular flow analysis of TSLP in HepG2 using 0.5 ug/10^6 cells of antibody TSLP (Clone: ABM27H9). Green represents isotype control; red represents anti-TSLP antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

WB (Western Blot) (Fig-1: Western blot analysis of TSLP. Anti-TSLPin antibody (Clone: ABM27H9) was used at 4 ug/ml on hHeart, hSKM and mouse embryonic Liver lysates.)

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TSLP, Monoclonal Antibody (Cat# AAA78289)

Full Name

Monoclonal Antibody to TSLP (Clone: ABM27H9)

Gene Names

CRLF2; CRL2; TSLPR; CRLF2Y

Reactivity

Mouse, Human

Applications

Flow Cytometry, Western Blot

Purity

Purified
Protein G Chromatography

Pricing

$215/0.025 mg $395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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WB (Western Blot) (Figure-3: Western blot analysis of MSI-2. Anti Msi-2 (Clone: ABM5A48) was used at 0.5 ug/ml in Recombinant and 2 ug/ml in SKBR3, MCF7 Lysates.)

FCM/FACS (Flow Cytometry) (Figure-2: Intra cellular flow analysis of Msi-2 on A431 cells using 2 ug/10^6 cells of Msi-2 antibody (Clone: ABM5A48). Green represents isotype control; red represents anti-Msi-2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody)

FCM/FACS (Flow Cytometry) (Figure-1: Intra cellular flow analysis of Msi-2 on HeLa cells using 2 ug/10^6 cells of Msi-2 antibody (Clone: ABM5A48). Green represents isotype control; red represents anti-Msi-2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

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MSi-2, Monoclonal Antibody (Cat# AAA78434)

Full Name

Monoclonal Antibody to MSi-2 (Clone: ABM5A48)

Gene Names

MSI2; MSI2H

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Purified
Protein G Chromatography

Pricing

$215/0.025 mg $395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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Application Data (Cell surface staining of THP1 cells. Green represents Isotype control. Red represents Anti-TSLP Receptor (AAA78228). 0.5 µg antibodies were used. Goat anti- mouse PE was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of TSLP Receptor. Anti- TSLP Receptor antibody (Clone: ABM28B6) was tested at 2 µg/ml on (1) THP-1 and (2) human heart lysate.)

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TSLP Receptor, Monoclonal Antibody (Cat# AAA78228)

Full Name

TSLP Receptor Monoclonal Antibody

Gene Names

CRLF2; CRL2; TSLPR; CRLF2Y

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Protein G Chromatography

Pricing

$215/0.025 mg $395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure-2: Intra Cellular flow analysis of ROR Gamma in PBMC (Lymphocyte gated) using 0.5 ug/10^6 cells of Anti-ROR Gamma. Green represents isotype control; red represents Anti- ROR Gamma antibody. Goat Anti-mouse PE conjugate was used as the secondary antibody.)

WB (Western Blot) (Figure-1: Western blot analysis of ROR Gamma. Anti- ROR Gamma antibody (Clone: ABM21H2) was used at 6 ug/ml on (1) A431, (2) PANC-1, (3) HepG2, (4) Hela, (5) A375 and (6) h Testis lysates.)

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ROR Gamma, Monoclonal Antibody (Cat# AAA78417)

Full Name

Monoclonal Antibody to ROR Gamma (Clone: ABM21H2)

Gene Names

RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Purified
Protein G Purification

Pricing

$215/0.025 mg $395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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IF (Immunofluorescence) (Figure 4. IF analysis of PNP using anti- PNP antibody (AAA125910).PNP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- PNP Antibody (AAA125910) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of PNP using anti-PNP antibody (AAA125910).PNP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PNP Antibody (AAA125910) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of PNP using anti-PNP antibody (AAA125910).PNP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PNP Antibody (AAA125910) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PNP using anti-PNP antibody (AAA125910).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human U87 whole cell lysatesLane 4: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PNP antigen affinity purified monoclonal antibody (Catalog # AAA125910) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PNP at approximately 32KD. The expected band size for PNP is at 32KD.)

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PNP, Monoclonal Antibody (Cat# AAA125910)

Full Name

Anti-PNP Antibody (monoclonal, 7G5)

Gene Names

PNP; NP; PUNP; PRO1837

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot)

WB (Western Blot)

IF (Immunofluorescence)

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IDH1, Monoclonal Antibody (Cat# AAA125497)

Full Name

Anti-IDH1 Antibody (Monoclonal, 16H7)

Gene Names

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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WB (Western Blot) (Detection of human SMAD3 by western blot. Samples: Whole cell lysate (50 ug) from PANC-1, HeLa, A-549, Jurkat, HEK293T, K-562, U2OS, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human SMAD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from PANC-1 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used for IP at 20 ul/mg lysate. SMAD3 was also immunoprecipitated by rabbit anti-SMAD3 antibody BL-1699B-2D12. For blotting immunoprecipitated SMAD3, AAA213605 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

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SMAD3, Monoclonal Recombinant Antibody (Cat# AAA213605)

Full Name

Rabbit anti-SMAD3 Recombinant Monoclonal Antibody [BLR144J]

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

Application Data (p21/WAF1 transfected cell lysate probed with Mouse anti Human p21/WAF1)

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p21/WAF1, Monoclonal Antibody (Cat# AAA49361)

Full Name

MOUSE ANTI HUMAN p21/WAF1

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Applications

Western Blot, Immunoprecipitation

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,735/5x0.1 mg

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Application Data (Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD3)

Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

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CD3, Monoclonal Antibody (Cat# AAA49610)

Full Name

MOUSE ANTI DOG CD3

Applications

Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry

Pricing

$490/0.1 mg $955/2x0.1 mg $1,400/3x0.1 mg $1,815/4x0.1 mg $2,000/5x0.1 mg

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WB (Western Blot) (Western blot of extracts from HeLa cells, using anti-SIK2, clone S15G10.)

ICC (Immunocytochemistry) (Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.)

IHC (Immunohistochemistry) (Anti-SIK2 / SNF1LK2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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SIK2 / SNF1LK2, Monoclonal Antibody (Cat# AAA51786)

Full Name

Mouse Monoclonal [clone S15G10] (IgG1) to Human SIK2 / SNF1LK2

Gene Names

SIK2; QIK; SNF1LK2; LOH11CR1I

Reactivity

Human

Applications

Immunoprecipitation, Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Affinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohistochemistry) (DAB staining on IHC-P. Samples: Human Brain Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Glioma Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Liver Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant HJV, Human.)

WB (Western Blot) (Western Blot: Sample: Rat Heart Tissue.)

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Hemojuvelin (HJV), Monoclonal Antibody (Cat# AAA146555)

Full Name

Monoclonal Antibody to Hemojuvelin (HJV)

Gene Names

HFE2; JH; HJV; RGMC; HFE2A

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$235/0.1 mL $300/0.2 mL $495/0.5 mL $605/1 mL $1,650/5 mL $3,210/2x5 mL $4,690/3x5 mL $6,085/4x5 mL $7,440/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Rat Liver Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemisry) (DAB staining on IHCP;Sample: Rat Testis Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Rat Kidney Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Rat Spleen Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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Interferon Gamma (IFNg), Monoclonal Antibody (Cat# AAA151439)

Full Name

Monoclonal Antibody to Interferon Gamma (IFNg)

Gene Names

Ifng; IFNG2

Reactivity

Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$205/0.1 mL $240/0.2 mL $390/0.5 mL $465/1 mL $1,255/5 mL $2,450/2x5 mL $3,580/3x5 mL $4,645/4x5 mL $5,685/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse AntiRat CD40L AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Rat Kidney Tissue; Primary Ab: 30ug/ml Mouse AntiRat CD40L Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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Cluster Of Differentiation 40 Ligand (CD40L), Monoclonal Antibody (Cat# AAA151485)

Full Name

Monoclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

Gene Names

Cd40lg; Cd40l; Tnfsf5

Reactivity

Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $290/0.2 mL $465/0.5 mL $580/1 mL $1,550/5 mL $3,015/2x5 mL $4,410/3x5 mL $5,720/4x5 mL $6,980/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHCP;Samples: Human Cerebellum Tissue;Primary Ab: 20ug/ml Mouse AntiHuman S100B AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohiostchemistry) (DAB staining on IHCP; Samples: Human Cerebrum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman S100B Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

WB (Western Blot)

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S100 Calcium Binding Protein B (S100B), Monoclonal Antibody (Cat# AAA151572)

Full Name

Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$220/0.1 mL $285/0.2 mL $455/0.5 mL $570/1 mL $1,520/5 mL $2,965/2x5 mL $4,330/3x5 mL $5,630/4x5 mL $6,835/5x5 mL

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DB (Dot Blot) (Histone H3K9me0 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 ug/mL. Lane 1: Histone H3 unmodified. Lane 2: Histone H3K4me3. Lane 3: Histone H3K9me3. Lane 4: Histone H3 K9ac. Lane 5: Histone H3 K14ac. Lane 6: Histone H3K4ac. Lane 7: Histone H3 S10ph.)

WB (Western Blot) (Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.)

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Histone H3K9me0, Monoclonal Antibody (Cat# AAA60019)

Full Name

Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6)

Reactivity

Human, Wide Range Predicted

Applications

Western Blot

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $705/0.1 mg $1,375/2x0.1 mg $2,005/3x0.1 mg $2,605/4x0.1 mg $3,015/5x0.1 mg

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Application Data (Histone H4K8ac antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K8ac antibody (mAb) was applied at a dilution of 0.7 ug/ml to MODified Histone Peptide Array tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K8ac antibody (mAb) for acetyl-Lys8 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K8ac antibody (mAb) at a dilution of 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide. Lane 9: acetyl-Lys20 peptide. Lane 10: unmodified Lys20 peptide. Lane 11: acetyl-Lys31 peptide. Lane 12: unmodified Lys31 peptide.)

WB (Western Blot) (Histone H4K8ac antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4K8ac (mAb) at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: cells treated with sodium butyrate.)

ChIP (Chromatin Immunoprecipitation) (Histone H4K8ac antibody (mAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4K8ac (mAb) or the equivalent amount of mouse IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

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Histone H4K8ac, Monoclonal Antibody (Cat# AAA60043)

Full Name

Histone H4K8ac antibody (mAb)(Clone MABI 0408)

Gene Names

HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116

Reactivity

Human, Wide Range Predicted

Applications

Western Blot, Dot Blot, Chromatin immunoprecipitation

Purity

Protein G Chromatography

Pricing

$725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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Application Data (Formalin-paraffin breast DCIS (ductal carcinoma in situ) stained with HER-2 Ab (HRB2/451).)

Application Data (Formalin-paraffin breast invasive carcinoma stained with HER-2 Ab (HRB2/451).)

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HER-2, Monoclonal Antibody (Cat# AAA62563)

Full Name

HER-2

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human.

Applications

Immunohistochemistry, Western Blot

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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IF (Immunofluorescence) (Immunofluorescence of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody at dilution 1:20, and DAPI(blue))

WB (Western Blot) (Western blot detection of PI3 Kinase p85 alpha in Jurkat,C6,CHO-K1 cell lysates using PI3 Kinase p85 alpha Rabbit mAb(1:1000 diluted).Predicted band size:84kDa.Observed band size:84kDa.)

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PI3 Kinase p85 alpha, Monoclonal Antibody (Cat# AAA178771)

Full Name

Recombinant PI3 Kinase p85 alpha Monoclonal Antibody

Gene Names

PIK3R1; p85; AGM7; GRB1; p85-ALPHA

Reactivity

Human, Rat, Hamster

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purified

Pricing

$255/0.05 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of APG5L/ATG5 (green) in MCF-7 using APG5L/ATG5 antibody at dilution 1:20, and DAPI(blue))

WB (Western Blot) (Western blot detection of APG5L/ATG5 Rabbit pA in K562,C6,Hela cell lysates using APG5L/ATG5 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:55kDa.)

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APG5L, Monoclonal Antibody (Cat# AAA178788)

Full Name

Recombinant APG5L Monoclonal Antibody

Gene Names

ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purified

Pricing

$255/0.05 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.