Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IHC (Immunohiostchemistry) (Fig: 2 Immunohistochemical analysis of p53 in human colon carcinoma using p53 antibody (Clone: BP53-12) at 1:100 dilution.)

WB (Western Blot) (Fig: 1 Western blot analysis of p53. Anti p53 antibody (Clone: BP53-12) was used at 1 ug/ml on 1) A431, 2) MCF7, 3) HEK293 lysates.)

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p53, Monoclonal Antibody (Cat# AAA78573)

Full Name

Monoclonal Antibody to p53 (Clone BP53-12)

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Protein G Chromatography

Pricing

$395/0.1 mg $765/2x0.1 mg $1,120/3x0.1 mg $1,450/4x0.1 mg $1,610/5x0.1 mg

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IHC (Immunohiostchemistry)

WB (Western Blot)

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S100A7, Monoclonal Antibody (Cat# AAA77810)

Full Name

S100A7, Human, mAb 9F3

Gene Names

S100A7; PSOR1; S100A7c

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunoassay, Immunohistochemistry

Pricing

$795/0.1 mg $1,540/2x0.1 mg $2,240/3x0.1 mg $2,910/4x0.1 mg $3,160/5x0.1 mg

IB (Immunoblot) (Immunoblotting of RL-ph2 recognizing M13 phage coat protein g8p at a 1:1000 dilution.)

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M13 phage coat protein g8p, Monoclonal Antibody (Cat# AAA77476)

Full Name

Mouse anti M13 phage coat protein g8p

Reactivity

M13 phage

Applications

Western Blot, Immunocytochemistry, Flow Cytometry

Purity

ProtG affinity purification

Pricing

$620/0.1 mg $1,200/2x0.1 mg $1,755/3x0.1 mg $2,275/4x0.1 mg $2,565/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

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PSD95, Monoclonal Antibody (Cat# AAA103280)

Full Name

PSD95 Antibody: Biotin

Gene Names

Dlg4; Dlgh4; PSD95; Sap90

Reactivity

Mouse, Rat, Bovine, Human

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Pricing

$555/0.1 mg $1,070/2x0.1 mg $1,565/3x0.1 mg $2,025/4x0.1 mg $2,190/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NPR3 protein using NPR3 antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of NPR3 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using NPR3 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NPR3 protein (Right) or empty vector (Left) detected with NPR3 antibody)

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NPR3, Monoclonal Antibody (Cat# AAA107261)

Full Name

NPR3 antibody

Gene Names

NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

NPR3 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PAICS protein using PAICS antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PAICS protein (Right) or empty vector (Left) detected with PAICS antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PAICS protein (red) or empty vector (blue) stained using PAICS antibody)

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PAICS, Monoclonal Antibody (Cat# AAA107537)

Full Name

PAICS antibody

Gene Names

PAICS; ADE2; AIRC; PAIS; ADE2H1

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

PAICS antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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Application Data (Abatacept bridging ELISA for Anti-Drug Antibody (ADA) assay developmentA microtiter plate was coated overnight with abatacept at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335, black), clone AbD37065ia (HCA336, red) or Mouse Anti-Human CD152 . Detection was performed using HRP conjugated abatacept followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)

Application Data (Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335) at a concentration of 2 ug/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)

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CD152, Monoclonal Antibody (Cat# AAA50478)

Full Name

MOUSE ANTI HUMAN CD152

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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WB (Western Blot) (Western blot analysis of Cox2 expression in A549 cell lysate.)

WB (Western Blot) (All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody (M00084-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTGS2.)

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Cox2, Monoclonal Antibody (Cat# AAA125127)

Full Name

Anti-Cox2 Monoclonal Antibody

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity-Chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western Blot analysis of FHL1 expression in Human fetal muscle lysate.)

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FHL1, Monoclonal Antibody (Cat# AAA125139)

Full Name

Anti-FHL1 Monoclonal Antibody

Gene Names

FHL1; KYOT; SLIM; FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity-Chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜. Data represents mean+/-SEM of triplicates from a representative of 3 independent experiments.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

Application Data (Published customer image: Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization. A) Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FceR? chain (right panel). B) Serum-opsonization inhibits the recognition of zymosan by both dectin-1 and dectin-2. NIH3T3 retrovirally transduced as indicated in the legend were incubated with zymosan that had been pre-incubated with serum at increasing concentrations and the interaction was monitored by flow cytometry. Data represents the mean+/-SEM of triplicates from a representative of 2 independent experiments. Data in (D) was analyzed by two-way ANOVA, with the significant Interaction statistics demonstrating the reduced binding of zymosan to the transduced cells after prior incubation with increasing concentrations of serum.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

Application Data (Mouse spleen stained wiith Rat anti Mouse Dectin-2)

Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/M˜ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜ from dectin-1-deficient mice. All bars show mean+/-95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (˜C') or the use of different cell lineages (˜L'). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

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DECTIN-2, Monoclonal Antibody (Cat# AAA49373)

Full Name

RAT ANTI MOUSE DECTIN-2

Gene Names

Clec4n; Nkcl; Clec6a; Clecsf10

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD63)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD63:FITC)

Application Data (Peripheral Human Monocytes stained with Mouse anti Human CD63:APC)

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CD63, Monoclonal Antibody (Cat# AAA49638)

Full Name

MOUSE ANTI HUMAN CD63:APC

Gene Names

CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30

Applications

Flow Cytometry

Pricing

$600/100 Tests $1,160/2x100 Tests $1,695/3x100 Tests $2,200/4x100 Tests $2,465/5x100 Tests

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Application Data (Rat peritoneal macrophages stained with Rat anti Mouse P2X7:Alexa Fluor 488)

Application Data (Rat peritoneal macrophages stained with Rat anti Mouse P2X7)

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P2X7, Monoclonal Antibody (Cat# AAA49756)

Full Name

RAT ANTI MOUSE P2X7

Gene Names

P2rx7; P2X7R; P2X(7); AI467586

Applications

Immunofluorescence, Flow Cytometry

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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IHC (Immunohistochemisry) (DAB staining on IHCP;Sample: Human Skeletal muscle Tissue; Primary Ab: 30ug/ml Mouse AntiHuman NTProANP AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Human Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse AntiHuman NTProANP AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Human Stomach Tissue; Primary Ab: 30ug/ml Mouse AntiHuman NTProANP Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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NTerminal ProAtrial Natriuretic Peptide (NTProANP), Monoclonal Antibody (Cat# AAA151546)

Full Name

Monoclonal Antibody to NTerminal ProAtrial Natriuretic Peptide (NTProANP)

Gene Names

NPPA; ANF; ANP; PND; ATFB6; CDD-ANF

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$215/0.1 mL $280/0.2 mL $440/0.5 mL $535/1 mL $1,445/5 mL $2,815/2x5 mL $4,115/3x5 mL $5,335/4x5 mL $6,495/5x5 mL

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WB (Western Blot) (Western Blot; Sample: Lane1: Human Serum; Lane2: Rat Cerebrum lysate Primary Ab: 3ug/ml Mouse AntiHuman APOA1 Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

IHC (Immunohistochemisry) (DAB staining on IHCP;Sample: Human Small intestine Tissue; Primary Ab: 10ug/ml Mouse AntiHuman APOA1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 10ug/ml Mouse AntiHuman APOA1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Human Kidney Tissue; Primary Ab: 10ug/ml Mouse AntiHuman APOA1 Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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Apolipoprotein A1 (APOA1), Monoclonal Antibody (Cat# AAA151549)

Full Name

Monoclonal Antibody to Apolipoprotein A1 (APOA1)

Reactivity

Human, Rat, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$215/0.1 mL $280/0.2 mL $440/0.5 mL $535/1 mL $1,445/5 mL $2,815/2x5 mL $4,115/3x5 mL $5,335/4x5 mL $6,495/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Human Cardiac Muscle Tissue; Primary Ab: 10ug/ml Mouse AntiHuman SEMA5B AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse AntiHuman SEMA5B Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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Semaphorin 5B (SEMA5B), Monoclonal Antibody (Cat# AAA151906)

Full Name

Monoclonal Antibody to Semaphorin 5B (SEMA5B)

Gene Names

SEMA5B; SemG; SEMAG

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $520/0.5 mL $625/1 mL $1,725/5 mL $3,365/2x5 mL $4,915/3x5 mL $6,375/4x5 mL $7,800/5x5 mL

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IHC (Immunohistochemistry) (CD163 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry) (CD163 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CD163 Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)

ICC (Immunocytochemistry) (CD163 Antibody-Anti-CD163 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.)

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CD163, Monoclonal Antibody (Cat# AAA162637)

Full Name

CD163 Mouse anti-Human Monoclonal (2F8) Antibody

Gene Names

CD163; M130; MM130

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence

Purity

Immunoaffinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Western Blot; Sample: Bovine Testis lysate Primary Ab: 2ug/ml Mouse Anti-human AMH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Testis TissuePrimary Ab: 30ug/ml Mouse Anti-human AMH AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemisry) (DAB staining on IHC-P;Sample: Rat Ovary TissuePrimary Ab: 30ug/ml Mouse Anti-human AMH AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohiostchemistry) (DAB staining on IHC-P;Sample: Rat Testis Tissue; Primary Ab: 30ug/ml Mouse Anti-human AMH AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: Rat Ovary Tissue; Primary Ab: 30ug/ml Mouse Anti-human AMH Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Anti-ullerian Hormone (AMH), Monoclonal Antibody (Cat# AAA152683)

Full Name

Monoclonal Antibody to Anti-ullerian Hormone (AMH)

Gene Names

AMH; MIF; MIS

Reactivity

Human, Rat, Bovine

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$305/0.1 mL $400/0.2 mL $720/0.5 mL $885/1 mL $2,485/5 mL $4,840/2x5 mL $7,075/3x5 mL $9,190/4x5 mL $11,215/5x5 mL

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Application Data (RNA pol II CTD monomethyl Lys7 antibody tested for specificity Biotinylated peptides containing various RNA Pol II CTD Lysine 7 modifications were captured onto wells of a Streptavidin-coated plate and then incubated with RNA Pol II CTD K7me1 antibody at 1 ug/mL. Peptide-bound antibody was detected using anti-rat IgG HRP followed by addition of peroxidase substrate and read at A405.)

IP (Immunoprecipitation) (RNA pol II CTD monomethyl Lys7 antibody tested by Immunoprecipitation. 5 ug of antibody was used to immunoprecipitate RNA pol II CTD monomethyl Lys7 from 300 ug of K562 nuclear extract, which was then run on SDS-PAGE and probed with the same antibody at 3 ug/ml.)

WB (Western Blot) (RNA pol II CTD monomethyl Lys7 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)

ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD K7me1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Raji cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10.9M sequence tags were mapped to identify RNA pol II binding sites across a region of chromosome 12.)

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RNA pol II CTD monomethyl Lys7, Monoclonal Antibody (Cat# AAA60095)

Full Name

RNA pol II CTD monomethyl Lys7 antibody (mAb)(Clone 1F5)

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Purity

Protein G Chromatography

Pricing

$250/0.01 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of BRAF protein in paraffin embedded Human colon tissue using BRAF antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BRAF protein (Right) or empty vector (Left) detected with BRAF antibody)

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BRAF, Monoclonal Antibody (Cat# AAA74874)

Full Name

BRAF antibody

Gene Names

BRAF; NS7; BRAF1; RAFB1; B-RAF1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

BRAF antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SATB1 mAb. [Lot No.3548D4_2a-1])

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-SATB1 monoclonal antibody.)

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SATB1, Monoclonal Antibody (Cat# AAA36528)

Full Name

Mouse monoclonal antibody Anti-Human SATB1

Reactivity

Human

Applications

Immunocytochemistry, Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

WB (Western Blot) (Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)

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HA-Tag, Monoclonal Antibody (Cat# AAA36624)

Full Name

HA-Tag Monoclonal Antibody

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$255/0.1 mL $500/2x0.1 mL $730/3x0.1 mL $940/5x0.1 mL $945/4x0.1 mL

Application Data

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Nesprin 1, Monoclonal Antibody (Cat# AAA59511)

Full Name

Mouse Anti-Nesprin 1 [MANNES1A(7A12)]

Gene Names

SYNE1; 8B; CPG2; ARCA1; EDMD4; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2

Reactivity

Mouse, Rat, Rabbit, Human

Applications

Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$650/0.1 mg $1,260/2x0.1 mg $1,845/3x0.1 mg $2,390/4x0.1 mg $2,640/5x0.1 mg

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Application Data ((0.01ug/ml) staining in MCF7(A) cells lysate, human lung(B) and human breast carcinoma(C) tissue lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)

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Mucin1, Monoclonal Antibody (Cat# AAA59577)

Full Name

Mucin1 Monoclonal Antibody [VU-3D1]

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein A or G purified

Pricing

$420/0.02 mg $560/0.05 mg $650/0.1 mg $1,260/2x0.1 mg $1,845/3x0.1 mg $2,390/4x0.1 mg $2,640/5x0.1 mg

SDS-PAGE (DS-PAGE Analysis Purified EBV Mouse Monoclonal Antibody. Confirmation of Purity and Integrity of Antibody.)

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EBV Early Antigens, Monoclonal Antibody (Cat# AAA62617)

Full Name

EBV Early Antigens

Reactivity

EBV

Applications

Immunofluorescence

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with EMA Monoclonal Antibody (SPM492).)

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MUC1 / EMA /CD227, Monoclonal Antibody (Cat# AAA62847)

Full Name

MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunofluorescence, Flow Cytometry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (MGN185+F5D))

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Myogenin, Monoclonal Antibody (Cat# AAA62503)

Full Name

Myogenin (Skeletal Muscle Marker) Mouse Monoclonal Antibody

Gene Names

MYOG; MYF4; myf-4; bHLHc3

Reactivity

Human, Mouse, Rat, Cat, Pig

Applications

Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Western blot analysis of Human Serum using TTR Mouse mAb diluted at 1:2000)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Thyroid Tissue using TTR Mouse mAb diluted at 1:200.)

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TTR, Monoclonal Antibody (Cat# AAA243666)

Full Name

TTR Monoclonal Antibody

Gene Names

TTR; CTS; CTS1; PALB; TBPA; HsT2651

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of SHP2 in paraffin-embedded Human breast cancer tissue using SHP2 Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of SHP2 in Hela,293 cell lysates using SHP2 Rabbit mAb(1:1000 diluted).Predicted band size:68kDa.Observed band size:68kDa.)

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SHP2, Monoclonal Antibody (Cat# AAA314264)

Full Name

SHP2 Rabbit mAb

Gene Names

PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using P70 S6 Kinase Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using P70 S6 Kinase Mouse mAb diluted at 1:200.)

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P70 S6 Kinase, Monoclonal Antibody (Cat# AAA309470)

Full Name

P70 S6 Kinase Mouse Monoclonal Antibody(1C7)

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)

WB (Western Blot) (Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1, 000 dilution.)

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CENPC, Monoclonal Antibody (Cat# AAA312655)

Full Name

CENPC Antibody

Gene Names

CENPC; MIF2; hcp-4; CENP-C; CENPC1

Reactivity

Human

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $470/0.1 mL $920/2x0.1 mL $1,340/3x0.1 mL $1,735/4x0.1 mL $1,940/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot) (Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela)

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BMAL1, Monoclonal Antibody (Cat# AAA312538)

Full Name

BMAL1 Antibody

Gene Names

ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $470/0.1 mL $920/2x0.1 mL $1,340/3x0.1 mL $1,735/4x0.1 mL $1,940/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Thyroid Tissue using TTR Mouse mAb diluted at 1:200)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using TTR Mouse mAb diluted at 1:200)

WB (Western Blot) (Western blot analysis of Human Serum using TTR Mouse mAb diluted at 1:2000)

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TTR, Monoclonal Antibody (Cat# AAA309529)

Full Name

TTR Mouse Monoclonal Antibody(1D7)

Gene Names

TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with LGR5/GPR49 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of LGR5/GPR49 on SH-SY-5Y lysates using anti-LGR5/GPR49 antibody at 1/1, 000 dilution.)

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LGR5/GPR49, Monoclonal Antibody (Cat# AAA312203)

Full Name

LGR5/GPR49 Antibody

Gene Names

LGR5; FEX; HG38; GPR49; GPR67; GRP49

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $470/0.1 mL $920/2x0.1 mL $1,340/3x0.1 mL $1,735/4x0.1 mL $1,940/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining VEGF Receptor 2 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining VEGF Receptor 2 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining VEGF Receptor 2 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

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VEGF 2, Monoclonal Antibody (Cat# AAA312210)

Full Name

VEGF Receptor 2 Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $470/0.1 mL $920/2x0.1 mL $1,340/3x0.1 mL $1,735/4x0.1 mL $1,940/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HeLa cells showing cytoplasmic staining.)

WB (Western Blot) (Western blot analysis of MST-3 expression in non-transfected 293T (A), human MST-3 transfected 293T (B) and A-431 (C) whole cell lysates.)

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MST3, Monoclonal Antibody (Cat# AAA311994)

Full Name

MST3 Antibody

Gene Names

STK24; MST3; STK3; MST3B; STE20

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IF (Immunofluorescence)

Application Data

IF (Immunofluorescence)

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Ly6G, Monoclonal Antibody (Cat# AAA197035)

Full Name

Anti-Mouse Ly6G In Vivo Antibody - Low Endotoxin (1A8)

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry

Purity

>95% by SDS-PAGE and HPLC
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Pricing

$560/5 mg $1,395/25 mg $2,180/50 mg $3,110/100 mg $6,060/2x100 mg $8,855/3x100 mg $11,500/4x100 mg $13,740/5x100 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of Hela tissue using HSP70 Monoclonal Antibody at dilution of 1:100.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus cancer tissue using HSP70 Monoclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of 1) Hela, 2) Mouse brain using HSP70 Monoclonal Antibody at dilution of 1:2000.)

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HSP70, Monoclonal Antibody (Cat# AAA171616)

Full Name

HSP70 Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Bax Monoclonal Antibody at dilution of 1:200.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Bax Monoclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of 1) Hela, 2) C2C12, 3) PC-12 cells using Bax Monoclonal Antibody at dilution of 1:1000.)

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Bax, Monoclonal Antibody (Cat# AAA173665)

Full Name

Bax Monoclonal Antibody

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Protein A Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Stomach Tissue))

IHC (Immunohiostchemistry) (DAB staining on IHC-P; Samples: Human Stomach Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Brain Natriuretic, Monoclonal Antibody (Cat# AAA141306)

Full Name

Monoclonal Antibody to Brain Natriuretic Peptide (BNP)

Gene Names

NPPB; BNP

Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$205/0.1 mL $240/0.2 mL $390/0.5 mL $465/1 mL $1,255/5 mL $2,450/2x5 mL $3,580/3x5 mL $4,645/4x5 mL $5,685/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Glioma Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant AREG, Human.)

WB (Western Blot) (Western Blot: Sample: Rat Placenta lysate; Primary Ab: 3ug/ml Mouse Anti-Human AREG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Amphiregulin (AREG), Monoclonal Antibody (Cat# AAA146470)

Full Name

Monoclonal Antibody to Amphiregulin (AREG)

Gene Names

AREG; AR; SDGF; AREGB; CRDGF

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$205/0.1 mL $240/0.2 mL $390/0.5 mL $465/1 mL $1,255/5 mL $2,450/2x5 mL $3,580/3x5 mL $4,645/4x5 mL $5,685/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of RSK1 p90 in paraffin-embedded Human Cholangiocarcinoma using RSK1 p90 Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of RSK1 p90 in Hela cell lysates using RSK1 p90 Rabbit mAb(1:500 diluted).Predicted band size:83kDa.Observed band size:90kDa.)

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RSK1 p90, Monoclonal Antibody (Cat# AAA314453)

Full Name

RSK1 p90 Rabbit mAb

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.