Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-NGF (PTR1353) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Claudin 2 (PT0311) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates of K562 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TGF ?1(PT2173) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PMEL (PT2221) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK20(ABT044) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (HaCat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK4(ABT061) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-E-Cadherin (ABT270) antibody. The HRP-conjugated Goat anti-M)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysate using Ikaros (C-terminus) mouse mAb.)
WB (Western Blot) (Western blot detection of Ikaros(C-terminus) in Ramos and Jurkat cell lysates using Ikaros(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry of HeLa cells using anti-Rab2 mouse mAb diluted 1:200)
WB (Western Blot) (Western blot detection of Rab2 in Hela.Ramos and K562 cell lysates and using Rab2 mouse mAb (1:800 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1(ABT-MLH1) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohiostchemistry) (IHC image of AAA243707 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA243707 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
IHC (Immunohiostchemistry) (Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 ug/ml. Commercially tested by LifeSpan BioSciences.)
FCM/FACS (Flow Cytometry) (Fig-3: Intra cellular flow analysis of RANKL in HepG2 using 0.5 ug/10^6 cells of antibody (Clone: ABM12D6). Green represents isotype control; red represents anti-RANKL antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
FCM/FACS (Flow Cytometry) (Fig-2: Intra cellular flow analysis of RANKL in Jurkat using 0.5 ug/10^6 cells of antibody (Clone: ABM12D6). Green represents isotype control; red represents anti-RANKL antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
WB (Western Blot) (Fig-1: Western blot analysis of RANKL. Anti-RANKL antibody (Clone: ABM12D6) was used at 2 ug/ml on hHeart lysate.)
FCM/FACS (Flow Cytometry) (Fig-2: Intracellular flow analysis of hTLR4 antibody in TLR4 Transfected Cell line using 2 ug/10^6 cells of hTLR4 antibody (Clone: ABM19C4). Green represents isotype control; red represents anti-hTLR4 antibody. Goat anti-mouse FITC conjugate was used as secondary antibody.)
FCM/FACS (Flow Cytometry) (Fig-1: Immunohistochemical analysis of TLR4 in human skin tissue using TLR4 antibody (Clone: ABM19C4) at 5 ug/ml.)
IHC (Immunohistochemistry) (Fig. 4: Immunoperoxidase staining of paraffin-embedded sections of human foreskin with mouse anti-human sFlt1-14 antibody. (D) Control; Fixation: 4% PFA (app. 25min); Dilution: 1:100-500. The staining in smooth muscle cells is visible.)
IF (Immunofluorescence) (Fig. 3: Immunofluorescence staining (green) of cryo-sections of human foreskin with mouse anti-human sFlt1-14 antibody [Cat# AAA79326] and counter staining of nuclei with Dapi (blue). (B) Control; Fixation: 4% PFA (app. 25min); Dilution: 1:500. Staining in smooth muscle cells, the perineurium of nerves and the Stratum basale of the epidermis as well as some scattered cells.)
ELISA (Fig. 2: Direct ELISA: recombinant human sFlt-1(D5) [Cat# sFlt-1 [Cat# and sFlt1-14 [Cat# INQUIRE] were coated with 1ug/ml in PBS. The sFlt1-14 specific mouse anti-human sFlt1-14 antibody generated against a peptide from the unique C terminal end was used with 2ug/ml, the Flt-1 specific mouse anti-human Flt-1 #EWI [Cat# recognizing all Flt-1 proteins was used as control [1ug/ml].)
WB (Western Blot) (Fig. 1: Western Blot Analysis of recombinant human sFlt-1(D5) [Cat# sFlt-1 [Cat# sFlt1-14 [Cat# INQUIRE], sKDR(D7) [Cat# and sFLT-4 [Cat# using a monoclonal mouse anti-human sFlt1-14 generated against a peptide from the unique C terminal end (left panel) and a monoclonal mouse anti-human Flt-1 [Cat# recognizing all Flt-1 proteins used as control.)
IHC (Immunohistochemisry) (Immunohistochemistry of S100A4 in paraffin-embedded Human lung cancer tissue using S100A4 Rabbit mAb at dilution 1:50)
WB (Western Blot) (Western blot detection of S100A4 in Hela cell lysates using S100A4 Rabbit mAb(1:500 diluted).Predicted band size:12kDa.Observed band size:12kDa.)
IF (Immunofluorescence) (Immunofluorescence of S1A4 (green) in hela using S1A4 Rabbit mAb at dilution 1/5, and DAPI(blue))
IF (Immunofluorescence) (Immunofluorescence analysis of Human lung cancer tissue using Lactoferrin Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using Lactoferrin Monoclonal Antibody at dilution of 1:200.)
Application Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag)
Application Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP)
Application Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 . Visualised using a LI-COR Odyssey infrared imaging system)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP)
Application Data (Detection of human C3 was performed using Mouse anti Human C3 at 0.5 ug/ml followed by Rabbit F(ab')2 anti Mouse IgG-HRP at 1:2000. Lane 2 shows the C3 alpha chain at molecular weight of approximately 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa. Lane 5 and 6 shows the mature C3 at molecular weight of approximately 186 kDa.Human plasma was run under reducing conditions at dilutions of 1/20 and 1/200 respectively in lanes 2 and 3, and under non-reducing conditions at dilutions of 1/20 and 1/200 in lanes 5 and 6, respectively. Lane 4 was left blank)
FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HeLa cells using anti-PKM2 antibody (AAA126886).Overlay histogram showing HeLa cells stained with AAA126886 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PKM2 Antibody (AAA126886, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 4. IF analysis of PKM2 using anti-PKM2 antibody (AAA126886).PKM2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-PKM2 Antibody (AAA126886) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemisry) (Figure 3. IHC analysis of PKM2 using anti-PKM2 antibody (AAA126886).PKM2 was detected in a paraffin-embedded section of differentiated adenocarcinoma of the human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PKM2 Antibody (AAA126886) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of PKM2 using anti-PKM2 antibody (AAA126886).PKM2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PKM2 Antibody (AAA126886) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of PKM2 using anti-PKM2 antibody (AAA126886).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human SH-SY5Y whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PKM2 antigen affinity purified monoclonal antibody (#AAA126886) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKM2 at approximately 60 kDa. The expected band size for PKM2 is at 60 kDa.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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