Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.)
WB (Western Blot) (Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsc70 protein using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant DSTN protein using DSTN antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of DSTN protein in paraffin embedded Adenocarcinoma of Human colon tissue using DSTN antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DSTN protein (Right) or empty vector (Left) detected with DSTN antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF antibody with DAB staining.)
WB (Western Blot) (Western Blot showing using BRAF antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).)
WB (Western Blot) (Western Blot showing BRAF antibody used against truncated recombinant Braf (1) and A431 cell lysate (2).)
IHC (Immunohistochemisry) (Immunohistochemical analysis of PGAM2 protein in paraffin embedded Human Kidney tissue using PGAM2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PGAM2 protein (Right) or empty vector (Left) detected with PGAM2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PGAM2 protein using PGAM2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RUVBL2 protein (Right) or empty vector (Left) detected with RUVBL2 antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of RUVBL2 protein in paraffin embedded Carcinoma of Human lung tissue using RUVBL2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant QPRT protein (Right) or empty vector (Left) detected with QPRT antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant QPRT protein (red) or empty vector (blue) stained using QPRT antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RASD2 protein using RASD2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RASD2 protein (Right) or empty vector (Left) detected with RASD2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RASD2 protein (red) or empty vector (blue) stained using RASD2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant GOLM1 protein using GOLM1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of GOLM1 protein in paraffin embedded Carcinoma of Human lung tissue using GOLM1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GOLM1 protein (Right) or empty vector (Left) detected with GOLM1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANXA11 protein using ANXA11 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANXA11 protein (Right) or empty vector (Left) detected with ANXA11 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BCKDK protein in paraffin embedded Human endometrium tissue using BCKDK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BCKDK protein (Right) or empty vector (Left) detected with BCKDK antibody)
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
BCKDK antibody was purified by affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry) (Staining of C57BL/6 splenocytes with CD3 antibody (FITC), NK1.1 antibody (APC), and 0.5 ug of CD160 antibody (PE). The histogram (right) demonstrates staining of CD160 on NK1.1+CD3- cells (red histogram) and NK1.1+CD3+ cells (black histogram), as gated in the dot plot (left).)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant POLR2J2 protein (Right) or empty vector (Left) detected with POLR2J2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using POLR2J2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ELK3 protein using ELK3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ELK3 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using ELK3 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ELK3 protein (Right) or empty vector (Left) detected with ELK3 antibody)
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using Thrombomodulin Recombinant Mouse Monoclonal Antibody (rTHBD/1591) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using C1QA Mouse Monoclonal Antibody (C1QA/2952)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified C1QA Mouse Monoclonal Antibody (C1QA/2952). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2952).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Kidney stained with C1QA Mouse Monoclonal Antibody (C1QA/2952).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBPA mouse mAb with DAB staining.)
WB (Western Blot) (Figure 1: Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
WB (Western Blot) (Western BlotPositive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, ntera-2 whole cell lysate, Mouse brain tissueAll lanes: PAK1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61, 62 kDaObserved band size: 61 kDa)
WB (Western Blot) (Myc-tagged fusion protein were subjected to SDS PAGE followed by western blot with AAA248000 (MYC tag antibody) at dilution of 1:10000)
IP (Immunoprecipitation) (IP Result of anti MYC tag (IP: AAA248000, 4ug; Detection: AAA248000 1:5000) with Transfected HEK-293 cells lysate 1000ug.)
ICC (Immunocytochemistry) (ICC staining alpha-tubulin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis on PC12 cell lysates using anti-alpha-tubulin mouse mAb.)
WB (Western Blot) (Detection of human E-Selectin/CD62E by western blot. Samples: Whole cell lysate (50 ug) from HUVEC-C cells untreated (-) or treated (+) with 10ng/mL TNFalpha. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (AAA213694 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human E-Selectin/CD62E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HUVEC-C cells treated with 10ng/mL TNFalpha. Antibodies: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (AAA213694 lot 1) used for IP at 6 ul/mg lysate. E-Selectin/CD62E was also immunoprecipitated by a second antibody against a different epitope of E-Selectin/CD62E (BL-2789A-4F3). For blotting immunoprecipitated E-Selectin/CD62E, AAA213694 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry) (Detection of human E-Selectin/CD62E by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (AAA213694). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human E-Selectin/CD62E by immunocytochemistry. Sample: FFPE section of HUVEC-C cells treated with TNF-alpha (left) and mock treated HUVEC-C cells (right). Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (AAA213694). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Detection of human E-Selectin/CD62E (shaded) in TNF-alpha treated HUVEC cells by flow cytometry. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (AAA213694) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (ICC staining RND3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RND3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of RND3 on HEK293 (1) and RND3-hIgGFc transfected HEK293 (2) cell lysate using anti-RND3 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of RND3 on human RND3 recombinant protein using anti-RND3 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SK-N-SH cells with GRIN3B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with GRIN3B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
WB (Western Blot) (Western blot analysis of GRIN3B on HEK293 (1) and GRIN3B-hIgGFc transfected HEK293 (2) cell lysate using anti-GRIN3B antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of GRIN3B on human GRIN3B recombinant protein using anti-GRIN3B antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with CD163 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD163 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD163 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD163 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD163 on human thymus tissue lysate using anti-CD163 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells using Bmi-1 Monoclonal Antibody (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using Bmi-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded cervical cancer tissues with DAB staining using Bmi-1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Bmi-1 Monoclonal Antibody against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using CD71 Monoclonal Antibody (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of MSCS cells using CD71 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded brain tissues with DAB staining using CD71 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using CD71 Monoclonal Antibody against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using Crk II Monoclonal Antibody (blue) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using Crk II Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded intima cancer tissues with DAB staining using Crk II Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Crk II Monoclonal Antibody against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using HSP27 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using HSP27 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) with DAB staining using HSP27 Monoclonal Antibody)
WB (Western Blot) (Western Blot analysis using HSP27 Monoclonal Antibody against HeLa (1). A549 (2). Jurkat (3). A431 (4). HEK293(5). HepG2 (6) and PC-12 (7) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using HSP60 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using HSP60 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) with DAB staining using HSP60 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using HSP60 Monoclonal Antibody against T47D (1). HeLa (2). HepG2 (3). A549 (4). Jurkat (5). HEK293 (6). NIH/3T3 (7). PC-12 (8) and Cos7 (9) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells using IL-34 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of U251 cells using IL-34 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using IL-34 Monoclonal Antibody against IL-34-hIgGFc transfected HEK293 cell lysate.)
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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