Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using CD71 Monoclonal Antibody (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of MSCS cells using CD71 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded brain tissues with DAB staining using CD71 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using CD71 Monoclonal Antibody against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using Crk II Monoclonal Antibody (blue) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using Crk II Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded intima cancer tissues with DAB staining using Crk II Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Crk II Monoclonal Antibody against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using GSK-3? mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot analysis of extracts from A549. Hela. 3T3 and Rat Brain cell lysates using GSK-3? mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using GSK-3?.GSK-3? Rabbit mAb was used for the western blot analysis (1:1000 diluted).)
ICC (Immunocytochemistry) (Immunocytochemistry staining of Jurkat cells fixed with -20? Ethanol and using anti-AMACR (C-terminus) mouse mAb (dilution 1:100).)
WB (Western Blot) (Western blot detection of AMACR(C-terminus) in Rat Liver lysates using AMACR(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size: 42KDa.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBPA mouse mAb with DAB staining.)
WB (Western Blot) (Figure 1: Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.)
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using Thrombomodulin Recombinant Mouse Monoclonal Antibody (rTHBD/1591) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human breast cancer tissue using Cytokeratin 6 Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using Cytokeratin 6 Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) Hela, 2) HepG2 cells using Cytokeratin 6 Monoclonal Antibody at dilution of 1:2000.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using HSC70 Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) Hela, 2)Mouse brain, 3) Rat brain using HSC70 Monoclonal Antibody at dilution of 1:2000.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human stomach cancer tissue using Ubiquitin Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Ubiquitin Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using Ubiquitin Monoclonal Antibody at dilution of 1:1000.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human Macrophages:RPE . Permeabilised with Leucoperm)
Application Data (Macrophages stained with Mouse anti Human Macrophages/ Monocytes using enhanced DAB in experimental allergic marmoset brain. Mag. X 400)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages . Permeabilised with Leucoperm)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC. Permeabilised with Leucoperm)
IF (Immunofluorescence) (ICC/IF analysis of PDCP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot) (Western blot analysis of STAT6 expression in Raji cell lysate (AAA46952).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT6)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat liver, using STAT6 Antibody(AAA46952)STAT6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-STAT6 Antibody (AAA46952)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of Cathepsin D expression in (1)MCF-7 cell lysate;(2)SKBR-3 cell lysate (AAA47053).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTSD)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human ovarian, using Cathepsin D Antibody(AAA47053)CTSD was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTSD Antibody (AAA47053)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using the Anti-Fc epsilon R antibody IE7 (AAA72131). U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of IE7 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of U937 cells using anti-Fc epsilon R antibody (AAA72131) IE7. Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of IE7 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL.Green: TIM-3 Antibody [1C6]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL.Green: TIM-3 Antibody [1C6]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD30 protein, His tagged protein PME100481 can bind Rabbit anti-CD30 monoclonal antibody (clone: DM103) in a linear range of 0.12-60 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD30 protein, His tagged protein PME100481 can bind Rabbit anti-CD30 monoclonal antibody (clone: DM103) in a linear range of 0.12-60 ng/ml.)
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ABCF2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2001C1-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ABCF2 mAb. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)
WB (Western Blot) (Detection of ABCF2 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CLNS1A mAb. [Lot No. 2187C2a-1])
WB (Western Blot) (Detection of CLNS1A by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2187C2a-1]Predicted molecular weight: 26 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-CLNS1A monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PPP1R13L mAb. [Lot No.2808C5a-1])
WB (Western Blot) (Detection of human PPP1R13L by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2808C5a-1]Predicted molecular weight: 89 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-PPP1R13L monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-UBP1 mAb. [Lot No. 327C1a-1])
WB (Western Blot) (Detection of human UBP1 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 327C1a-1]Predicted molecular weight: 60 kDa)
Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-UBP1 monoclonal antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CXCR4 antibody (AAA36798) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-POU2F1 mAb. [Lot No. POU5I097-1])
WB (Western Blot) (Detection of human POU2F1 by Western blot.Samples: Whole cell lysate (50 ug) from HEK293 cells. [Lot No. POU5I097-1]Predicted molecular weight: 76 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-POU2F1 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZNF161 mAb. [Lot No. ZNF5J141-2])
WB (Western Blot) (Detection of ZNF161 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. ZNF5J141-2]Predicted molecular weight: 56 kDa)
Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-ZNF161 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-EIF2S1 mAb. [Lot No. EIF2S1A2B8-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-EIF2S1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. EIF2S1A2B8-1])
WB (Western Blot) (Detection of EIF2S1 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. EIF2S1A2B8-1]Predicted molecular weight: 36 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-EIF2S1 monoclonal antibody.)
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRPF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2093C5a-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRPF1 mAb. [Lot No. 2093C5a-1]Predicted molecular weight: 138 kDa)
WB (Western Blot) (Detection of BRPF1 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug) and rat NIH3T3 (M, 50 ug) cells. [Lot No. 2093C5a-1]Predicted molecular weight: 138 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-BRPF1 monoclonal antibody.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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