Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)
WB (Western Blot) (Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.)
Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜. Data represents mean+/-SEM of triplicates from a representative of 3 independent experiments.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Application Data (Published customer image: Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization. A) Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FceR? chain (right panel). B) Serum-opsonization inhibits the recognition of zymosan by both dectin-1 and dectin-2. NIH3T3 retrovirally transduced as indicated in the legend were incubated with zymosan that had been pre-incubated with serum at increasing concentrations and the interaction was monitored by flow cytometry. Data represents the mean+/-SEM of triplicates from a representative of 2 independent experiments. Data in (D) was analyzed by two-way ANOVA, with the significant Interaction statistics demonstrating the reduced binding of zymosan to the transduced cells after prior incubation with increasing concentrations of serum.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Application Data (Mouse spleen stained wiith Rat anti Mouse Dectin-2)
Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/M˜ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜ from dectin-1-deficient mice. All bars show mean+/-95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (˜C') or the use of different cell lineages (˜L'). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Application Data (Published Customer Image:Mouse Anti Porcine granulocytes antibody, clone 2B2 used to identify mature neutrophils in porcine blood by flow cytometery in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 expressed on both immature and mature porcine neutrophils.Image caption:Gating strategy.Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSChigh 6D10+ 2B2-) and mature neutrophils (CD172a+ SSChigh 6D10+ 2B2+).From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.Delayed development of systemic immunity in preterm pigs as a model for preterm infants.Sci Rep. 2016 Nov 10;6:36816.)
Application Data (Figure A. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse anti Pig granulocytes . All experiments performed on red cell lysed porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer)
Application Data (Figure A. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse IgG1 isotype control detected with FITC conjugated Goat anti Mouse IgG1 Figure B. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse anti Pig granulocytes detected with FITC conjugated Goat anti Mouse IgG1 . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell monocytes. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-AHNAK mAb. [Lot No. 2014C3a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-AHNAK monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX27 mAb. [Lot No. 2251C2a-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DDX27 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2251C2a-1])
WB (Western Blot) (Detection of DDX27 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. 2251C2a-1]Predicted molecular weight: 89 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-DDX27 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BrdU antibody (Green) and TriMethyl-Histone H3-K27 antibody (Red). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse jejunum using BrdU antibody at dilution of 1:300 (40x lens).)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human DNAM-1 protein, mFc-His tagged protein PME100050 can bind Rabbit anti-DNAM-1 monoclonal antibody ( clone: DM95) in a linear range of 0.64-80 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human DNAM-1 protein, mFc-His tagged protein PME100050 can bind Rabbit anti-DNAM-1 monoclonal antibody ( clone: DM95) in a linear range of 0.64-80 ng/ml.)
IF (Immunofluorescence) (ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : THP-1 cell lysate)
ICC (Immunocytochemistry) (Localization of human GTF2IRD1 in HeLa cells.HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc)
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells transfected with vector (lanes 1, 3) or myc-tagged GTF2IRD1 (lanes 2, 4) was incubated with anti-GTF2IRD1 (BMR00190) conjugated with protein G sepharose. Immunocomplex was analyzed by Western blot with anti-mPredicted molecular weight: 106 kDa)
WB (Western Blot) (Detection of human GTF2IRD1 by Western blot.Sample: Whole cell lysate was obtained from HeLa cells transiently transfected with vector (lanes 1, 3) or myc-tagged human GTF2IRD1 (lanes 2, 4). Primary antibodies: anti-myc tag (1 ug/ml) (lanes 1, 2) or antPredicted molecular weight: 106 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-GTF2IRD1 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BXDC1 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 2099C7a-1])
WB (Western Blot) (Detection of human BXDC1 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2099C7a-1]Predicted molecular weight: 35 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-BXDC1 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZNF38 mAb. [Lot No. 3418F1a-1])
WB (Western Blot) (Detection of human ZNF38 by Western blot.Samples: Whole cell lysate (25 ug) from HEK293 cells. [Lot No. 3418F1a-1]Predicted molecular weight: 53 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ZNF38 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HDLBP mAb. [Lot No. 2404C3a-1])
WB (Western Blot) (Detection of HDLBP by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2404C3a-1]Predicted molecular weight: 23 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-HDLBP monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HCCA2 mAb. [Lot No. 2400C3a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-HCCA2 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F5 mAb. [Lot No.220D1a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-E2F5 monoclonal antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of over-expressed DDDDK-tagged protein in HeLa cell incubated with DDDDK antibody. A mock served as negative control and over-expressed Hela cell lysate served as positive control.)
WB (Western Blot) (Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)
ELISA (Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA (Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40985. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40985) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SAMHD1 protein using SAMHD1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SAMHD1 protein (Right) or empty vector (Left) detected with SAMHD1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PAICS protein using PAICS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PAICS protein (Right) or empty vector (Left) detected with PAICS antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PAICS protein (red) or empty vector (blue) stained using PAICS antibody)
PAICS antibody was purified by affinity chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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