Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
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IHC (Immunohistochemistry) (Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).Commercially tested by LifeSpan BioSciences.)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
WB (Western Blot) (Western Blot Lane1: Rat Brain Tissue Lane2: Rat Liver Tissue Primary Ab: 3ug/mL Rabbit Anti-Human bTG Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab)
WB (Western Blot) (Detection of human S100A9 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HL-60, HEK293T, MUTZ-3, and THP-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH antibody .)
IHC (Immunohistochemistry) (Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of spleen. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry) (Detection of human S100A9 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of MUTZ-3 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human S100A9 by immunocytochemistry. Sample: FFPE section of NK-92 cells. Antibody: Rabbit anti-S100A9 recombinant monoclonal antibody (AAA213638 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using PAPP-A Mouse Monoclonal Antibody (PAPPA/2718) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS_PAGE (SDS-PAGE Analysis Purified PAPP-A Mouse Monoclonal Antibody (PAPPA/2718). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemisry) (Formalin-fixed, paraffin-embedded human Placenta stained with PAPP-A Mouse Monoclonal Antibody (PAPPA/2718).)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with PAPP-A Mouse Monoclonal Antibody (PAPPA/2718).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with PAPP-A Mouse Monoclonal Antibody (PAPPA/2718).)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-colon tissue. 1.Kif 7 Monoclonal Antibody(3F8)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.C/EBP ? Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). Kif 7 Monoclonal Antibody(3F8)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)
IHC (Immunohistochemistry) (IHC staining of Mouse Kidney tissue. diluted at 1:200.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
WB (Western Blot) (Western blot analysis of HepG2. diluted at 1:2.000.)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) Rat Testis tissue. 3) Raw264.7. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
Application Data (The picture was kindly provided by our customer)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse SpleenTissue using CD 25 Mouse mAb diluted at 1:500.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.CD25 Monoclonal Antibody(Q22) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). ?-tubulin Monoclonal Antibody(M7)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)
IHC (Immunohiostchemistry) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) Mouse Brain tissue. 3) Rat Brain tissue. diluted at 1:5000.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using TRP2/DCT Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using TRP2/DCT Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Human Brain. 2) Mouse Brain. 3) Rat Brain Tissue Lysate using TRP2/DCTMouse Monoclonal mAb diluted at 1:5.000.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human KidneyTissue using Src Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Src Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Hela Cell. 2) 293T Cell Lysate using SrcMouse Monoclonal mAb diluted at 1:2.000.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using PPAR ? Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using PPAR ? Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Hela Cell. 2) 293T Cell Lysate using PPAR?Mouse Monoclonal mAb diluted at 1:2.000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung CarcinomaTissue using Myd 88 Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung CarcinomaTissue using Myd 88 Mouse Monoclonal antibody diluted at 1:200.)
ICC (Immunocytochemistry) (Immunocytochemistry of HeLa cells using anti-CDK5(N-terminus) mouse mAb diluted 1:150)
WB (Western Blot) (Western blot detection of CDK5(N-terminus) in 3T3.Hela.PC-12.COS7.Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-eEF2 mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot detection of eEF2 in HL-60.Jurkat.SHSY-5Y.U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using RanBP9 mouse mAb (1:300).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using RanBP9 mouse mAb.)
WB (Western Blot) (Western blot detection of RanBP9 in Jurkat.MOLT-4 and CEM cell lysates and using RanBP9 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 95KDa.)
IF (Immunofluorescence) (Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at -20? and using anti-PKM2 mouse mAb (dilution 1:500).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using PKM2.)
WB (Western Blot) (Western blot detection of PKM2 in HCT116.COS7.K562.A549 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using Hsp27 mouse mAb (1:300).)
WB (Western Blot) (Western blot detection of Hsp27 in Hela.HsHela.A431.COS7.Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of K562 cell lysates using CHD4 mouse mAb.)
WB (Western Blot) (Western blot detection of CHD4 in K562.HelaNE.HL-60.MOLT-4.Jurkat and 293 cell lysates using CHD4 mouse mAb (1:1000 diluted).Predicted band size:260KDa.Observed band size:260KDa.)
WB (Western Blot) (Western blot detection of LIN28A in LIN28B and LIN28A recombinant antigen fragments and using LIN28A mouse mAb (1:1000 diluted).)
WB (Western Blot) (Western blot detection of LIN28 in NTERA and F9 cell lysates using LIN28A mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells surface-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (dilution 1:100).)
WB (Western Blot) (Western blot detection of Noggin in CHO-K1 cell lysates over-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:37KDa.)
WB (Western Blot) (Western blot detection of SMYD4 in Hela.MCF7 and A431 cell lysates using SMYD4 mouse mAb (1:200 diluted).Predicted band size:89KDa.Observed band size:89KDa.)
WB (Western Blot) (Western blot detection of SMYD4 in 40ug Hela.40ug MCF7 and 40ug MDA-MB-468 cell lysates using SMYD4 mouse mAb (1:100 diluted).Predicted band size:89KDa.Observed band size:89KDa.)
WB (Western Blot) (Western blot detection of Caspase-9 in CHO-K1 cell lysate(B) and CHO-K1 transfected by Caspase-9(A) cell lysate using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49/37KDa.)
WB (Western Blot) (Western blot detection of Caspase-9 in A549.MCF7.Jurkat.K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using Hsp60 mouse mAb (dilution 1:100).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Hsp60 mouse mAb.)
WB (Western Blot) (Western blot detection of Hsp60 in Hela.Raji.Jurkat.C6.C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
Application Data (Recombinant Anthrax Protective Antigen used as Coating antigen.followed by different dilutions of supernatants which includes secreted Anti-Anthrax Protective Antigen Human antibody.HRP conjugated Anti-Human IgG(201068) Mouse mAb was used for coloring.)
Application Data (Recombinant Anthrax Protective Antigen used as Coating antigen.followed by Anti-Anthrax Protective Antigen Human antibody and then incubated with Anti-Human IgG(201068) Mouse mAb. HRP conjugated goat anti Mouse IgG was used for coloring.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using p90RSK mouse mAb.)
WB (Western Blot) (Western blot detection of p90RSK in COS7.3T3.Jurkat.Hela and A549 cell lysates using p90RSK mouse mAb(dilution 1:1000).Predicted band size:90KDa.Observed band size:90KDa.)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20? and using anti-eIF2? mouse mAb (dilution 1:200).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using eIF2?.)
WB (Western Blot) (Western blot detection of eIF2? in K562.COS7.3T3. C2C12.MCF-7.C6 and Hela cell lysates using eIF2? mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.)
WB (Western Blot) (Western blot detection of Vimentin in Molt-4.K562.COS7.Jurkat.Hela and Vimentin negative cell (Daudi.Ramos.Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Raji cell lysates using SHP-1 mouse mAb.)
WB (Western Blot) (Western blot detection of SHP-1 in Human tonsil.Daudi.Jurkat and Raji cell lysates using SHP-1 mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells using anti-keratin8/18 antibody (dilution 1:200).)
WB (Western Blot) (Western blot detection of keratin8/18 in Hela. A549. PC-3 and SKOV3 cell lysates using keratin8/18 antibody (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
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What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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