Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Standard Curve (Sample) (Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein ); using Capture Antibody Mouse monoclonal [1A8-C5-B4] to Human Serum Albumin at 8 ug/ml and Detector Antibody Rabbit polyclonal antiserum to Human Serum Albumin.)
WB (Western Blot) (Western blot detection of Human Serum Albumin in 0.5nl human serum and 5ng ALB Recombinant antigens cell lysates using Human Serum Albumin mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red. 1/100 dilution). followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control. normal mouse IgG.)
ICC (Immunocytochemistry) (Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.)
WB (Western Blot) (Western blot detection of SMAD5 (C-terminus) in Hela.Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.)
WB (Western Blot) (Western blot detection of GTF2H1 in Hela.Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20? and using SirT2 (200474.dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045.dilution 1:200) Rabbit pAb (red).)
WB (Western Blot) (Western blot detection of SirT2 in PC-12. Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39.43KDa. Observed band size:39.43KDa.)
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human breast carcinoma stained with HER-2/c-erbB-2 Recombinant Rabbit Monoclonal Antibody (ZR5).)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # RHD27102 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # RHD27102 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # RHD27102 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # RHD27102 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
Bioactivity (Detects Human LGALS3 in indirect ELISAs.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Catalase in paraffin-embedded Human breast cancer tissue using Catalase Rabbit mAb at dilution 1:100)
WB (Western Blot) (Western blot detection of Catalase in K562,Hela cell lysates using Catalase Rabbit mAb(1:1000 diluted).Predicted band size:60kDa.Observed band size:60kDa.)
WB (Western Blot) (Western Blot analysis of Recombinant DENV (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (Domain III, His Tag) (with 30ng) using Anti-Dengue virus DENV-2 (Strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (Domain III) Monoclonal Antibody at dilution of 1:2000.)
WB (Western Blot) (Western blot analysis of HAPLN1 expression in (1) Caco2 cell lysate; (2) Mouse spleen lysate (AAA124485).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAPLN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HAPLN1)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using HAPLN1 Antibody (AAA124485)HAPLN1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HAPLN1 Antibody (AAA124485)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Application Data (Published customer image: Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?+/-?0.21% (n?=?3). Average CD45+: 0.19?+/-?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:Alexa Fluor488)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:FITC)
Application Data (Published customer image: Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?+/-?0.21% (n?=?3). Average CD45+: 0.19?+/-?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.)
IF (Immunofluorescence) (Immunofluorescence analysis of RelB was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton„¢ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti RelB antibody at 1.0 ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with an Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody for 45 minutes at room temperature (Panel A: green). Nuclei (Panel B: blue) were counterstained with DAPI and F-actin (Panel C: red) with rhodamine phalloidin. Panel D is a merged image showing nuclear localization. Panel E is a no primary antibody control. The images were captured at 60x magnification)
WB (Western Blot) (Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB at 1-2 ug/ml in 5 % skimmed milk at 4 degree C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection)
WB (Western Blot) (Cell lysates of NIH3T3 (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Calmodulin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohiostchemistry) (Anti-Calmodulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry) (Anti-Calmodulin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
FCM/FACS (Flow Cytometry) (Staining of normal human peripheral blood cells with 0.5 ug of Purified Mouse IgG2b isotype control (open histogram) or Purified anti-human CD1d (51.1) (colored histogram) followed by Biotin Anti-Mouse IgG and Streptavidin-PE. Cells in the lymphocyte gate were used for analysis.)
IHC (Immunohistochemistry) (Anti-CD1D antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)
WB (Western Blot) (Western blot analysis using TGFBR3 mouse mAb against Jurkat (1), HeLa (2), MCF-7 (3), F9 (4), SK-N-SH (5), and NIH3T3 (6) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green).Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HAS2 mouse mAb with DAB staining.)
WB (Western Blot) (Western blot analysis using HAS2 mouse mAb against NTERA-2 (1),HEK293 (2) cell lysate.)
WB (Western Blot) (Figure 1: Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa))
IHC (Immunohistochemistry) (Figure 1: Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 mouse mAb with DAB staining.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lymph tissue (A), glioma tissue (B) and cerebellum tissue (C), showing membrane localization using Dynamin1 mouse mAb with DAB staining)
WB (Western Blot) (Figure 1: Western blot analysis using Dynamin1 mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).)
Biotin Linked Monoclonal Antibody to Leukemia Inhibitory Factor (LIF)
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human, Pig
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.