Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7365, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7365, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA41007, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA41007, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CTCF mAb. [Lot No. 252C3a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-CTCF monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NAB1 mAb. [Lot No. 2607C2a-11])
WB (Western Blot) (Detection of NAB1 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2607C2a-1]Predicted molecular weight: 54 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-NAB1 monoclonal antibody.)
ELISA (Titration ELISA of anti-HIV-1 p24 antibody PM-6335 with 100 ng of recombinant HIV-1 p24 protein.)
WB (Western Blot) (Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6335 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX6 mAb. [Lot No. PAX5I326-2])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-PAX6 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NARS mAb. [Lot No. NARS2G5-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HT1080 cells was incubated with anti-NARS mAb. [Lot No. NARS2G5-1]Predicted molecular weight: 62 kDa)
WB (Western Blot) (Detection of NARS by Western blot.Samples: Whole cell lysate from human HT1080 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. NARS2G5-3]Predicted molecular weight: 62 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-NARS monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TAF15 mAb. [Lot No.TAF15B11A6-2])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-TAF15 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-USF2 mAb. [Lot No. USF2E12D9-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-USF2 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LSM10 mAb. [Lot No.2522C1a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-LSM10 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-JMJD2A mAb. [Lot No.3559D5a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-JMJD2A monoclonal antibody.)
IP (Immunoprecipitation) (Immunoprecipitating FNTB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissueAll lanes: FNTB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49, 44 kDaObserved band size: 49 kDa)
WB (Western Blot) (SKOV-3 cells were subjected to SDS PAGE followed by western blot with AAA248034 (PAX8 Antibody) at dilution of 1:2000)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using AAA248034 (PAX8 Antibody) at dilution of 1:800)
ICC (Immunocytochemistry) (ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PAX8 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PAX8 on SKOV-3 cells lysates using anti-PAX8 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Liver Arginase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Liver Arginase on human liver tissue lysate using anti-Liver Arginase antibody at 1/500 dilution.)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
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What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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