Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)
WB (Western Blot) (Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.)
WB (Western Blot) (Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)
WB (Western Blot) (Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PAICS protein using PAICS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PAICS protein (Right) or empty vector (Left) detected with PAICS antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PAICS protein (red) or empty vector (blue) stained using PAICS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SERPINA5 protein (Right) or empty vector (Left) detected with SERPINA5 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SERPINA5 protein using SERPINA5 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PAICS protein using PAICS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PAICS protein (Right) or empty vector (Left) detected with PAICS antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PAICS protein (red) or empty vector (blue) stained using PAICS antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RanGAP1 protein using RanGAP1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of RanGAP1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using RanGAP1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RanGAP1 protein (Right) or empty vector (Left) detected with RanGAP1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PGAM2 protein (Right) or empty vector (Left) detected with PGAM2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PGAM2 protein using PGAM2 antibody)
IHC (Immunohistochemisry) (Immunohistochemical staining using PTPRE antibodyImmunohistochemical analysis of PTPRE protein in paraffin embedded Carcinoma of Human bladder tissue using PTPRE antibody)
IF (Immunofluorescence) (Immunofluorescent staining using PTPRE antibodyImmunofluorescent staining of COS7 cells transiently transfected with recombinant PTPRE protein using PTPRE antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis using PTPRE antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant PTPRE protein (red) or empty vector (blue) stained using PTPRE antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SLFNL1 protein (Right) or empty vector (Left) detected with SLFNL1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SLFNL1 protein using SLFNL1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDE4B protein using PDE4B antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of PDE4B protein in paraffin embedded Human pancreas tissue using PDE4B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDE4B protein (Right) or empty vector (Left) detected with PDE4B antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ITM2B protein in paraffin embedded Human Kidney tissue using ITM2B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ITM2B protein (Right) or empty vector (Left) detected with ITM2B antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of RNPEP protein in paraffin embedded Adenocarcinoma of Human breast tissue using RNPEP antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RNPEP protein (Right) or empty vector (Left) detected with RNPEP antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNPEP protein (red) or empty vector (blue) stained using RNPEP antibody)
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
RNPEP antibody was purified by affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry) (Staining of mouse platelets with CD61 antibody (PE) and 0.125 ug of Rat IgG1 K Isotype Control (left) or 0.125 ug of CD41 antibody (right).)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SH2D2A protein (Right) or empty vector (Left) detected with SH2D2A antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SH2D2A protein (red) or empty vector (blue) stained using SH2D2A antibody)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human DNAM-1 protein, mFc-His tagged protein PME100050 can bind Rabbit anti-DNAM-1 monoclonal antibody ( clone: DM95) in a linear range of 0.64-80 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human DNAM-1 protein, mFc-His tagged protein PME100050 can bind Rabbit anti-DNAM-1 monoclonal antibody ( clone: DM95) in a linear range of 0.64-80 ng/ml.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SAMHD1 protein using SAMHD1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SAMHD1 protein (Right) or empty vector (Left) detected with SAMHD1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BIN3 protein using BIN3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BIN3 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using BIN3 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BIN3 protein (Right) or empty vector (Left) detected with BIN3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CHEK2 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using CHEK2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ARFGAP1 protein using ARFGAP1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ARFGAP1 protein in paraffin embedded Human lymphoma tissue using ARFGAP1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARFGAP1 protein (Right) or empty vector (Left) detected with ARFGAP1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant GTF2F1 protein using GTF2F1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GTF2F1 protein (Right) or empty vector (Left) detected with GTF2F1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant HDAC1 protein using HDAC1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HDAC1 protein (Right) or empty vector (Left) detected with HDAC1 antibody)
HDAC1 antibody was purified by affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry) (Fig-2: Cell surface Flow analysis of CD28 in PBMC (lymphocytes gated) using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
FCM/FACS (Flow Cytometry) (Figure-2: Flowcytometric analysis of Rat NKG2A/C/E antibody. Anti- Rat NKG2A/C/E was tested in 293 transfected with NKG2A/C/E. Solid line represent secondary control and dotted line represent Anti- Rat NKG2A/C/E antibody.)
FCM/FACS (Flow Cytometry) (Figure-1: Flowcytometric analysis of Rat NKG2A/C/E antibody. Anti- Rat NKG2A/C/E was tested in rat splenocytes. Green line represent Isotope control and red line represent Anti- Rat NKG2A/C/E antibody. Goat anti-Rat FITC was used as secondary antibody)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of PD-1 in overexpressing HEK293 cells using PD-1 antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in human lymph node tissue with PD-1 antibody at 5 μg/mL. Corner Box: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of PD-1 in human lymph node tissue with PD-1 antibody at 20 μg/mL.Green: PD1 Antibody [10B3]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-1 in in overexpressing HEK293 cells with PD-1 antibody at 20 μg/mL.Green: PD1 Antibody [5D3]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in overexpressing HEK293 cells with PD-1 antibody at 10 μg/mL. Corner Box: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL.Green: TIM-3 Antibody [3G7]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL.Green: TIM-3 Antibody [3G7]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
ELISA (Figure 3 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, 35-580. An ELISA was performed using human SARS-CoV nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.)
ELISA (Figure 2 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, 35-580. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 16 ng/mL to 1000 ng/mL.)
WB (Western Blot) (Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of humannucleocapsid recombinant protein.Antibodies: SARS-CoV-2 nucleocapsid, 35-580, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2ug/mL)
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 10 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 μg/mL.Red: PDL1 Antibody [1D7] (RF16033)Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 μg/mL.Red: PDL1 Antibody [1D7] (RF16033)Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5ug/mL.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1ug/mL.)
ELISA (Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA (Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40984. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40984) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A6]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6333, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6333, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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