Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (JCHAIN/Ig J Chain Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.)
WB (Western Blot) (CTH/Cystathionase Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTH.)
Application Data (CTH/Cystathionase Antibody-Western blot of extracts (35ug) from 9 different cell lines by using anti-CTH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
IHC (Immunohiostchemistry) (Immunohistochemistry of Visfatin in paraffin-embedded Human lung cancer tissue using Visfatin Rabbit mAb at dilution 1:50)
WB (Western Blot) (Western blot detection of Visfatin in Hela,A549,HL-60,U2OS,C6 cell lysates using Visfatin Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.)
IF (Immunofluorescence) (Immunofluorescence of p53 (green) in MCF-7 using p53 antibody at dilution 1:20, and DAPI(blue))
WB (Western Blot) (Western blot detection of p53 in Jurkat cell lysates using p53 Rabbit mAb(1:500 diluted).Predicted band size:44kDa.Observed band size:53kDa.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle)
WB (Western Blot) (Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).)
WB (Western Blot) (HeLa cells were subjected to SDS PAGE followed by western blot with AAA247937 (HSP60 Antibody) at dilution of 1:20000)
IP (Immunoprecipitation) (IP Result of anti-HSP60 (IP: AAA247937, 5ug; Detection: AAA247937 1:2000) with mouse liver tissue lysate 4000ug.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using AAA247937 (HSPD1 Antibody) at dilution of 1:50)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-liver tissue. 1.CD21 Monoclonal Antibody(2C5)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1.CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (IHC staining of human tonsil tissue. diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.CD1 Monoclonal Antibody(9H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.CD1 Monoclonal Antibody(9H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human-tonsils using antibody diluted at 1:50.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CD1 Monoclonal Antibody(9H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.CD16 Monoclonal Antibody(Q32) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of 1) Jurkat. 2) K562. diluted at 1:2000.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1.CD15 Monoclonal Antibody(Q89) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat-heart tissue. 1.Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-kidney tissue. 1.Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1.Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.Brp44L Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) 3T3. 3) Raw264.7. 4) Rat Brain. 5) Rat Kidney. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
WB (Western Blot) (Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
Application Data (The picture was kindly provided by our customer)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using NFAT5 Mouse Monoclonal antibody diluted at 1:500.)
WB (Western Blot) (Western blot analysis of 1)Hela Cell. 2) Mouse Brain. 3) Rat Brain Tissue Lysate using NFAT5Mouse Monoclonal mAb diluted at 1:2.000.)
WB (Western Blot) (Western blot analysis of 1)PC12 Cell. 2) Mouse Brain Tissue Lysate using Tyrosine HydrolaseMouse Monoclonal mAb diluted at 1:2.000.)
WB (Western Blot) (Western blot analysis of 1)HepG2 Cell. 2) HepG2 Treated with CoCl2 cell Lysate using HIF-1?Mouse Monoclonal mAb diluted at 1:2.000.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using STAT3Mouse mAb diluted at 1:200.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using STAT3Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot detection of STAT3 in human breast cancer cell line MCF-7(A). T47D(B). MDA-MB-231(C) and Cal51 (D) using STAT3 mouse mAb (AAA320494. 1:2000 diluted).Predicted band size: 86kDa.Observed band size:86kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot) (Western blot analysis of 1) Rat Heart Tissue. 2)Mouse Heart Tissue. 3) Hela with STAT3 Mouse mAb diluted at 1:2.000.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Pancreatic Carcinoma using Cyclophilin BMouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma using Cyclophilin BMouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1) Hela. 2)Jurkat. 3)293T. 4)Rat Liver Tissue. 5) 3T3. 6) HepG2 with Cyclophilin B Mouse mAb diluted at 1:2.000.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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