Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using Prohibitin Monoclonal Antibody (blue) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Prohibitin Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded rectum cancer tissues with DAB staining using Prohibitin Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Prohibitin Monoclonal Antibody against A431 (1). MCF-7 (2). Jurkat (3). HeLa (4). HepG2 (5). A549 (6). NIH/3T3 (7). Cos7 (8) and PC-12 (9) cell lysate.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of PC12 cells using Reg I? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human Pancreas tissues with AEC staining using Reg I? Monoclonal Antibody.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using Rsk-2 Monoclonal Antibody against HeLa (1). MCF-7 (2). and HepG2 (3) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using RUNX1 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using RUNX1 Monoclonal Antibody against Jurkat cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) with DAB staining using CDC27 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using CDC27 Monoclonal Antibody against CDC27-hIgGFc transfected HEK293 cell.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using Daxx Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of PANC-1 cells using Daxx Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot) (Western Blot analysis using Daxx Monoclonal Antibody against K562 cell lysate (1).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded placenta tissues (left) and ovarian cancer (right) with DAB staining using E7 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using E7 Monoclonal Antibody against recombinant protein.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using E-cadherin Monoclonal Antibody (green) and negative control (purple).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining using E-cadherin Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using E-cadherin Monoclonal Antibody against LNCAP (1).A431 (2). DU145 (3). PC-3 (4). MCF-7 (5). PC-12 (6). NIH/3T3 (7). C6 (8) and COS7 (9) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using eIF4E Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of GC-7901 cells using eIF4E Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) with DAB staining using eIF4E Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using eIF4E Monoclonal Antibody against HeLa (1). HEK293 (2) and K562 (3) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung cancer (A). colon cancer (B). breast cancer (C) and rectal cancer(D) with DAB staining using EP-CAM Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using EP-CAM Monoclonal Antibody against HTC116 (1) and T47D (2) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and breast carcinoma tissue (right). showing cytoplasmic and membrane location with DAB staining using ErbB-3 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ErbB-3 Monoclonal Antibody against truncated Trx-ErbB-3 recombinant protein (1) and MBP-ErbB-3 (aa1175-1275) recombinant protein (2).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using Fibrinogen ? Monoclonal Antibody (blue) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using Fibrinogen ? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded cerebellum tissues with DAB staining using Fibrinogen ? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Fibrinogen ? Monoclonal Antibody against recombinant protein.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using TBLR1 mouse mAb (dilution 1:200).)
IHC (Immunohistochemistry) (IHC of paraffin-embedded huma breast cancer using anti-TBLR1 mouse mAb diluted 1/500-1/1000)
WB (Western Blot) (Western blot detection of TBLR1 in Mouse brain.Mouse heart and K562 cell lysates using TBLR1 mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60Kda.)
WB (Western Blot) (Western blot detection of TBLR1 in Hela and K562 cell lysates using TBLR1 mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60Kda.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.)
WB (Western Blot) (Western blot detection of IkB-alpha(N-terminus) in Jurkat and Hela cell lysates using IkB-alpha(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)
WB (Western Blot) (Western blot detection of IkB-alpha(N-terminus) in 3T3 cell lysate using IkB-alpha(N-terminus) mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Peptide Affinity-Chromatography
Pricing
WB (Western Blot) (Figure 1: Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
IHC (Immunohistochemisry) (IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysateAll lanes: SF3B1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 146, 17 kDaObserved band size: 130 kDa)
IF (Immunofluorescence) (BF0229 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-mouse IgG (H+L) Ab, diluted at 1/600, was used as secondary Ab.)
WB (Western Blot) (Figure 1: Western blot analysis using LAMB1 mouse mAb against truncated LAMB1-His recombinant protein (1).)
WB (Western Blot) (Figure 1: Western blot analysis using anti-CDC25C mAb against Hela (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.)
WB (Western Blot) (Western Blot Analysis of HCT116 Cell lysate using Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).)
SDS-PAGE (SDS-PAGE Analysis of Purified Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).)
Anti-Mouse CD8 In Vivo Antibody - Low Endotoxin (YTS-169)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Purity
>95% by SDS-PAGE and HPLC This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin (HKSP)
Purity
>95% by SDS-PAGE and HPLC This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
IHC (Immunohiostchemistry) (Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5). Commercially tested by LifeSpan BioSciences.)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Immunoprecipitation, Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
IgG fraction
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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