Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Seasonal Influenza A Hemagglutinin antibody recognizes seasonal influenza A (H1N1), but not swine-origin influenza A (S-OIV, H1N1), Hemagglutinin protein in ELISA.)
Application Data (Recombinant seasonal Influenza HA (lane A) or swine-origin Influenza HA (lane B) were probed (1 μg/mL) using monoclonal seasonal Influenza A HA.)
IF (Immunofluorescence) (Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.Red: ORAI1 Antibody [3F6H5]Blue: DAPI staining)
IHC (Immunohistochemistry) (Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.)
IHC (Immunohiostchemistry) (Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.)
ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with abs at decreasing concentrations.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [4C7] antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)
IF (Immunofluorescence) (Immunofluorescence of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL.Green: PD1 Antibody [4C7]Blue: DAPI staining)
IHC (Immunohiostchemistry) ((A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [4C7] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)
ELISA (Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml.Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
IHC (Immunohistochemisry) (Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.)
IF (Immunofluorescence) (Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml.Green: TIGIT Antibody [10B1] (AAA40889)Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
ELISA (Figure 2 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, 35-712. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugateat 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.)
WB (Western Blot) (Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, 35-712, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ?g/mL Lane 2: 1?g/mL)
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
PD-1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5ug/mL.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1ug/mL.)
ELISA (Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40978. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (AAA40978) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40978 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.)
ELISA (Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1ug/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and AAA40978 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.)
SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [RBD-2B9]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) S1 RBD Antibody [RBD-2B9] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA (Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40986. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40986) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)
ELISA (Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, AAA40987. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA40987) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.)
SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A9]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6333, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6333, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA40998, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA40998, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7371, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7371, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
IF (Immunofluorescence) (ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence) (ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IHC (Immunohistochemisry) (Anti-Bcl-2 antibody (monoclonal), AAA44907, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry) (Anti-Bcl-2 antibody (monoclonal), AAA44907, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot) (Anti-Bcl-2 antibody (monoclonal), AAA44907, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Small Intestine Tissue LysateLane 4: Rat Liver Tissue Lysate)
IHC (Immunohistochemistry) (Anti-CEA antibody (monoclonal), AAA44890, IHC(P)IHC(P): Human Rectal Cancer Tissue)
IHC (Immunohistochemisry) (Anti-CEA antibody (monoclonal), AAA44890, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry) (Anti-CEA antibody (monoclonal), AAA44890, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot) (Anti-CEA antibody (monoclonal), AAA44890, Western blottingLane 1: Recombinant Human CEA Protein 10ngLane 2: Recombinant Human CEA Protein 5ngLane 3: Recombinant Human CEA Protein 2)
Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Application Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC)
Application Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Azide Free)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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