Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of SiHa cells using anti- IDH2 antibody (AAA125904).Overlay histogram showing SiHa cells stained with AAA125904 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (AAA125904, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 2. IF analysis of IDH2 using anti- IDH2 antibody (AAA125904).IDH2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- IDH2 Antibody (AAA125904) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of IDH2 using anti-IDH2 antibody (AAA125904).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Sw620 whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: human HEPG2 whole cell lysatesLane 5: human Jurkat whole cell lysatesLane 6: rat heart tissue lysatesLane 7: rat liver tissue lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse heart tissue lysatesLane 10: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (Catalog # AAA125904) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDH2 at approximately 45KD. The expected band size for IDH2 is at 45KD.)
Application Data (Published customer image: Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
Application Data (Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor 647)
Application Data (Published customer image: Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
WB (Western Blot) (Western blot analysis using APOA1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY APOA1 cDNA (2).)
WB (Western Blot) (Detection of human SIRP alpha by western blot. Samples: Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)
IP (Immunoprecipitation) (Detection of human SIRP alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used for IP at 20 ul per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody [BL-565-1D6]. For blotting immunoprecipitated SIRP alpha, AAA213547 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry) (Detection of human SIRP alpha by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SIRP alpha by immunocytochemistry. Sample: FFPE section of Malme-3M cells. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
WB (Western Blot) (Detection of human Senataxin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, SW620, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used for IP at 6 ul/mg lysate. Senataxin was also immunoprecipitated by a previous lot of this antibody (AAA213548 lot 1) and a second antibody against a different epitope of Senataxin . For blotting immunoprecipitated Senataxin, AAA213548 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry) (Detection of human Senataxin (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-Senataxin recombinant monoclonal (AAA213548) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Detection of human TFEB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of mouse TFEB by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used for IP at 20 ul/mg lysate. TFEB was also immunoprecipitated by a previous lot of this antibody (AAA213560 lot 1) and a second antibody against a different epitope of TFEB . For blotting immunoprecipitated TFEB, AAA213560 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry) (Detection of human TFEB (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
ELISA (Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.)
FCM/FACS (Flow Cytometry) (FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF299-1).)
WB (Western Blot) (Western blot analysis of human Pref-1using anti-DLK1 (human), mAb (PF299-1) at 1: 2, 000 dilution. A.. 1. Human Pref-1 Fc protein. 2. Transfected human Pref-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B.. 1. Human Pref-1 Fc protein. 2. Mouse Pref-1 Fc protein. 3. Human DNER Fc protein. 4. Human DLL1 Fc protein. 5. Human DLL3 Fc protein. 6. Human DLL4 Fc protein. 7. Human Jagged1 Fc protein. 8. Human FGF23 Fc protein.)
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.