Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded ovarian cancer (left) and cerebellum tissues (right) with DAB staining using Msi2 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Msi2 Monoclonal Antibody against NTERA-2 (1). SW620 (2) and T47D (3) cell lysate.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209)-hIgGFc using MuSK Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung cancer (A). muscles (B) and breast cancer (C) with DAB staining using MuSK Monoclonal Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and breast carcinoma tissue (right). showing nuclear location with DAB staining using N-CoR Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using N-CoR Monoclonal Antibody against truncated Trx-N-CoR recombinant protein (1).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain (left) and breast carcinoma (right). showing cytoplasmic and membrane localization with DAB staining using PAR4 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using PAR4 Monoclonal Antibody against full-length Trx-PAR4 recombinant protein (1) and HeLa cell lysate (2).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Lovo cells using PRMT4 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using PRMT4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using PRMT4 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using PRMT4 Monoclonal Antibody against MCF-7 (1). HeLa (2). NIH/3T3 (3). HL-60 (4). LNcap (5). Jurkat (6). PC-3 (7). Cos7 (8). and PC-12 (9) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of SK-BR-3 cells using PTH/PTHrP-R Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human kidney tissues with DAB staining using PTH/PTHrP-R Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using PTH/PTHrP-R Monoclonal Antibody against PTH1R-hIgGFc transfected HEK293 cell lysate.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using Raf-B Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human testis tissues with AEC staining using Raf-B Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Raf-B Monoclonal Antibody against HeLa (1). HL60 (2). HepG2 (3) and NIH/3T3 (4) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using Rictor Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Rictor Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) with DAB staining using Rictor Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Rictor Monoclonal Antibody against HeLa (1). PANC-1 (2). MOLT4 (3). HepG2 (4) and HEK293 (5) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue (A). lymphoid follicles tissue (B) and interbrain tissue (C). showing cytoplasmic localization with DAB staining using S-100 ? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using S-100 ? Monoclonal Antibody against truncated S-100 ? recombinant protein.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain. cerebellun with DAB staining using S-100 ? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using S-100 ? Monoclonal Antibody against full-length S-100 ? recombinant protein.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of PANC-1 cells using SorLA Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human cerebrum tissues with DAB staining using SorLA Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using SorLA Monoclonal Antibody against truncated SorLA recombinant protein.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using FoxD3 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using FoxD3 Monoclonal Antibody against NTERA-2 (1). HUVE-12 (2). HEK293 (3). HeLa (4). Jurkat (5). K562 (6). RAW264.7 (7). NIH/3T3 (8). and COS7 (9) cell lysate.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of L-02 cells using GPI Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human Kidney tissues with AEC staining using GPI Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using GPI Monoclonal Antibody against HepG2 (1). SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma (left) and skin carcinoma (right). showing cytoplasmic localization with DAB staining using GSK3? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using GSK3? Monoclonal Antibody against truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using HIF-1? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) with DAB staining using HIF-1? Monoclonal Antibody.)
Application Data (Sun. Lei. et al. "Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia." American journal of cancer research 5.9 (2015): 2626.)
WB (Western Blot) (Western Blot analysis using HIF-1? Monoclonal Antibody against Cos7 (1). HeLa (2). Jurkat (3). RAJI (4) and NIH/3T3 (5) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of PANC-1 cells using Inhibin ? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using Inhibin ? Monoclonal Antibody against mouse spermary (1) tissues lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using LEDGF Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using LEDGF Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using LEDGF Monoclonal Antibody against HepG2 (1). Jurkat (2). K562 (3). Cos7 (4). PC-12 (5). HeLa (6). and NIH/3T3 (7) cell lysate.)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20? and using BiP/GRP78 mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot analysis of extracts from 3T3.A549.C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.)
IP (Immunoprecipitation) (Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis of K562 cells stained with CHD3 (red. 1/100 dilution). followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control. normal mouse IgG.)
IHC (Immunohiostchemistry) (IHC of paraffin-embedded human colon using anti-CHD3 (C-terminus) diluted 1/500-1/1000.)
WB (Western Blot) (Western blot detection of CHD3 (C-terminus) in Y79 and Raji cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 226KDa.Observed band size: 260KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.)
WB (Western Blot) (Western blot detection of CHD3 (C-terminus) in NTERA2.3T3.C6.K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.)
ELISA (Indirect ELISA assay for anti-Human Serum Albumin mouse mAb.Antigen coating concentration: 8ug/ml.)
WB (Western Blot) (Western blot detection of Human Serum Albumin in 0.5nl human serum and 10ng ALB Recombinant antigens cell lysates using Human Serum Albumin mouse mAb (1:2000 diluted).Predicted band size:67KDa.Observed band size:67KDa.)
WB (Western Blot) (Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
WB (Western Blot) (Western blot detection of DSBA in AKT1.AKT2.AKT3 and DSBA recombinant antigen fragments using DSBA mouse mAb (1:1000 diluted).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Cyclin H mouse mAb.)
WB (Western Blot) (Western blot detection of Cyclin H in Jurkat.K562 and A431 cell lysates using Cyclin H mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-PKM2 mouse mAb (dilution 1:400).)
WB (Western Blot) (Western blot detection of PKM2 in COS7.PC12.C6.3T3 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using Pyruvate Dehydrogenase E2 mouse mAb (1:300).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb.)
WB (Western Blot) (Western blot detection of Pyruvate Dehydrogenase E2 in Jurkat.A549.U251.F9.Lncap and Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (1:1000 diluted).Predicted band size:69KDa.Observed band size:69KDa.)
WB (Western Blot) (Western blot detection of AKT1 in CHO-K1 cell lysate(B) and CHO-K1 transfected by AKT1(A) cell lysate using AKT1 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
WB (Western Blot) (Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000).with super ECL.Predicted band size:60KDa.Observed band size:60KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using CBR1 mouse mAb (1:100).)
WB (Western Blot) (Western blot detection of CBR1 in Hela.A431 and MDA-MB-468 cell lysates using CBR1 mouse mAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:30KDa.)
WB (Western Blot) (Western blot detection of cIAP2 in Ramos.COS7.Raji and HeLa cell lysates using cIAP2 antibody(1:1000 diluted). Exposion time: 5min. Predicted band size: 68KDa.Observed band size:72KDa.)
WB (Western Blot) (Western blot detection of c-IAP2 in Ramos.COS7.Raji and Daudi cell lysates using c-IAP2 mouse mAb (1:1000 diluted).Predicted band size: 68KDa.Observed band size:72KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using CDX2 mouse mAb (1:100).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysate using CDX2 mouse mAb.)
WB (Western Blot) (Western blot detection of CDX2 in Hela.COS7 and K562 cell lysates using CDX2 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using Smad2 mouse mAb (dilution 1:100).)
WB (Western Blot) (Western blot detection of Smad2 in Hela.A431.Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
WB (Western Blot) (Western blot detection of Smad2 in 3T3 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
WB (Western Blot) (Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP mouse mAb (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.)
WB (Western Blot) (Western blot analysis of extracts from CHO-K1 cells. transfected with a human pDYKDDDDK-CMV2-BRAP construct (A) or transfected with a human pDYKDDDDK-CMV2 construct (B). using BRAP mouse mAb (1:1000 diluted).)
WB (Western Blot) (Western blot detection of MEK-2 in THP-1.Jurkat.Raji.3T3 and Hela cell lysates using 310001(MEK-2 rabbit pAb.1:1000 diluted).then incubated with 201069(Rabbit IgG mAb.1:50000 diluted).Anti-mouse IgG. HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.)
WB (Western Blot) (Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using Mouse Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:1000 diluted).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of CHO-K1 cell lysates transfected with HA-tagged NGAL using HA-Tag Rabbit pAb (390001.1:100 diluted)(lane 2). Lane 1 contains rabbit IgG IP control lysate. All lanes are probed with HA-Tag Rabbit pAb (390001.1:1000 diluted) as the primary antibody. Panel A incubated with secondary antibodies Anti-Rabbit whole IgG. HRP-linked Antibody for detecting.Panel B incubated with Anti-Rabbit IgG (Heavy-Chain Specific) mAb(1:50000 diluted) and the Anti-mouse whole IgG. HRP-linked Antibody was used for detecting.)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
WB (Western Blot) (Western blot detection of RBBP5 in C2C12.THP-1.PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.)
WB (Western Blot) (Western blot analysis of extracts from PC12.C6.Ramos.Jurkat and Hela cell lysates using MKK3/6 mouse mAb (1:1000 diluted).Predicted band size:40KDa.Observed band size:40KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Lamin B1 mouse mAb (dilution 1:100).)
WB (Western Blot) (Western blot detection of Lamin B1 in COS7. Molt-4. A549 and 3T3 cell lysates using Lamin B1 mouse mAb(dilution 1:500).Predicted band size:68kDa.Observed band size:68kDa.)
Application Data (Ge. Lu. et al. "PRMT5 promotes epithelial?mesenchymal transition via EGFR???catenin axis in pancreatic cancer cells." Journal of cellular and molecular medicine 24.2 (2020): 1969-1979.)
Application Data (0.5ug HA fusion protein+ Primary antibody dilution at 1:10000)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-GST-Pi (ABT-GSTP1) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Stathmin (ABT266) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53(ABT002) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (PC-12 whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Collagen alpha-1(I) chain(ABT162) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistochemistry) (Human prostate adenocarcinoma tissue was stained with anti-AR(ABT-AR) antibody.)
IHC (Immunohiostchemistry) (Human prostate adenocarcinoma tissue was stained with anti-AR(ABT-AR) antibody.)
SDS-PAGE (Whole cell lysates of LnCap were separated by 8% SDS-PAGE, and the membrane was blotted with anti-AMACR antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p57kip2 (ABT-P57)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-amyloid-Alpha-beta (PTR2007) antibody. The HRP-conjugated Goat anti-M)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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