Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Overlay histogram showing Hela cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Cdk5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cdk5 on Hela cell lysates using anti-Cdk5 antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Survivin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Survivin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Survivin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Survivin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GRK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-GRK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GRK2 on Hela cell lysates using anti-GRK2 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells with WSTF antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of WSTF on SiHa cell lysates using anti-WSTF at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1, 000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3)
Application Data (Figure 2. A: The scheme of Anti-BCMA BiTE molecule. B: Tumor cell killing assay. NCI-H929 cells (stably transfected with luciferase), were incubated with freshly isolated human PBMC, and different concentration of BiTE antibodies constructed from rabbit Anti-Human BCMA/TNFRSF17 Clone DM4 (red line), or BB2121 originated huC11D5.3 clone (blue line) or a no BCMA binding clone as negative control (black line). After 48hrs incubation, the viable NCI-H929 tumor cells were measured by luciferase activity assay.)
Application Data (Figure 1. The basic principle of BiTE cell killing assay. The BiTE molecule can effectively bring T cells to tumor target cells and stimulate tumor cell killing activity of T cells.)
IHC (Immunohistochemisry) (Immunohistochemistry of Cyclin A2 in paraffin-embedded Human lung cancer tissue using Cyclin A2 Rabbit mAb at dilution 1/50)
IF (Immunofluorescence) (Immunofluorescence of Cyclin A2 (green) in hela using Cyclin A2 Rabbit mAb at dilution 1/5, and DAPI(blue))
WB (Western Blot) (Western blot detection of Cyclin A2 in Jurkat,Hela cell lysates using Cyclin A2 Rabbit mAb(1:500 diluted).Predicted band size:49kDa.Observed band size:49kDa.)
WB (Western Blot) (Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 ug) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry) (Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used for IP at 6 ul/mg lysate. PDGFR beta was also immunoprecipitated by a second antibody against a different epitope of PDGFR beta (Antibody 2). For blotting immunoprecipitated PDGFR beta, AAA213658 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry) (Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, KG-1, Daudi, THP-1, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used for IP at 20 ul/mg lysate. SMARCA2/BRM was also immunoprecipitated by a second antibody against a different epitope of SMARCA2/BRM . For blotting immunoprecipitated SMARCA2/BRM, AAA213699 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
FCM/FACS (Flow Cytometry) (Detection of human SMARCA2/BRM (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, OVCAR-8, SK-MEL-28, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human p16INK4a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used for IP at 12 ul/mg lysate. p16INK4a was also immunoprecipitated by a second antibody against a different epitope of p16INK4a (RMAB3177B-3C3). For blotting immunoprecipitated p16INK4a, AAA213707 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry) (Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of Hek293T cells. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using PAPP-A Mouse Monoclonal Antibody (PAPPA/2717) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified PAPP-A Mouse Monoclonal Antibody (PAPPA/2717). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with PAPP-A Mouse Monoclonal Antibody (PAPPA/2717).)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2923) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2923). Confirmation of Purity and Integrity of Antibody.)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
FCM/FACS (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed MOLT4 cells. TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
SDS-PAGE (SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to TIGIT (TIGIT/3017). Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017).)
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human pancreas stained with Chromogranin A Recombinant Rabbit Monoclonal Antibody (CHGA/4544R).)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using MCAM Mouse Monoclonal Antibody (MCAM/3046). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified MCAM Mouse Monoclonal Antibody (MCAM/3046). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Melanoma stained with MCAM Mouse Monoclonal Antibody (MCAM/3046).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Melanoma stained with MCAM Mouse Monoclonal Antibody (MCAM/3046).)
WB (Western Blot) (Western Blot Analysis of human kidney tissue lysate using Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R).)
SDS-PAGE (SDS-PAGE Analysis Purified Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R). Confirmation of Integrity and Purity of Antibody)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human breast carcinoma stained with Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of lysates from 1) MCF7. 2) Hela. 3) 293T cells. ?Green? primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. ?Red? Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot) (Western blot analysis of lysates from 1) MCF7. 2) Hela. 3) 293T cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot) (Western blot analysis of Hela. diluted at 1:1000.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Raji cell lysates using BTK mouse mAb.)
WB (Western Blot) (Western blot detection of BTK in Daudi.Jurkat(BTK negative).K562.Ramos and Raji cell lysates using BTK mouse mAb (1:1000 diluted).Predicted band size:77KDa.Observed band size:77KDa.)
Application Data (The picture was kindly provided by our customer. Primary antibody was diluted at 1:2000. Loading control antibody was diluted at 1:20000)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD63 (ABT420) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Glycophorin A (ABT-GYPA) antibody. The HRP-conjugated Goat anti-M)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (CPTC-ANXA1-1).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (CPTC-ANXA1-1). Confirmation of Purity and Integrity.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with Annexin A1 Mouse Monoclonal Antibody (CPTC-ANXA1-1).)
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using MyoD1 Recombinant Rabbit Monoclonal Antibody (MYOD1/2075R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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