Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human PD-L1 protein, mFc His tagged protein PME100023 can bind Rabbit anti-PD-L1 monoclonal antibody (clone: DM124) in a linear range of 0.1-15 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human PD-L1 protein, mFc His tagged protein PME100023 can bind Rabbit anti-PD-L1 monoclonal antibody (clone: DM124) in a linear range of 0.1-15 ng/ml.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human TNFa , His tagged protein PME100076 can bind Anti-TNFa Neutralizing antibody (AAA47502) in a linear range of 0.3-500 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human TNFa , His tagged protein PME100076 can bind Anti-TNFa Neutralizing antibody (AAA47502) in a linear range of 0.3-500 ng/ml.)
IF (Immunofluorescence) (Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92. Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.)
WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TBXAS1 protein using TBXAS1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of TBXAS1 protein in paraffin embedded Human liver tissue using TBXAS1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TBXAS1 protein (Right) or empty vector (Left) detected with TBXAS1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SCHIP1 protein (Right) or empty vector (Left) detected with SCHIP1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SCHIP1 protein using SCHIP1 antibody)
WB (Western Blot) (Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Ischemic fresh brain tissue. Species: Rat. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Alexa Fluor 546 Goat Anti-mouse (Red) at 1:500 for 1 hour at RT. Localization: Cerebral Cortex. Courtesy of: Dr. Yi Yang, U. New Mexico.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x. With anti-microbial.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RALBP1 protein using RALBP1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of RALBP1 protein in paraffin embedded Adenocarcinoma of Human colon tissue using RALBP1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RALBP1 protein (Right) or empty vector (Left) detected with RALBP1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant VWA5A protein using VWA5A antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of VWA5A protein in paraffin embedded Carcinoma of Human lung tissue using VWA5A antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VWA5A protein (Right) or empty vector (Left) detected with VWA5A antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using VWA5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NPR3 protein using NPR3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of NPR3 protein in paraffin embedded Human liver tissue using NPR3 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NPR3 protein (Right) or empty vector (Left) detected with NPR3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of HDHD1 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using HDHD1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HDHD1 protein (Right) or empty vector (Left) detected with HDHD1 antibody)
WB (Western Blot) (Western Blot analysis using PPME1 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PPME1 protein (Right) or empty vector (Left) detected with PPME1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining using PPME1 antibodyImmunofluorescent staining of COS7 cells transiently transfected with recombinant PPME1 protein using PPME1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis using PPME1 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant PPME1 protein (red) or empty vector (blue) stained using PPME1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ZFP36 protein (Right) or empty vector (Left) detected with ZFP36 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant ZFP36 protein (red) or empty vector (blue) stained using ZFP36 antibody)
IHC (Immunohistochemistry) (IHC analysis of SOX9 using anti-SOX9 antibody. SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.)
IF (Immunofluorescence) (Antibody)
IHC (Immunohistochemistry) (IHC analysis of SOX9 using anti-SOX9 antibody. SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemisry) (IHC analysis of SOX9 using anti-SOX9 antibody.SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of SOX9 using anti-SOX9 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human CACO-2 whole cell lysates,Lane 3: human SW620 whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SOX9 antigen affinity purified monoclonal antibody at 1:500 overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.)
WB (Western Blot) (Western blot analysis of SOX9 expression in SW480 cell lysate (AAA46893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX9)
WB (Western Blot) (Western blot analysis of MHC class 1 expression in A549 cell lysate (AAA46900).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-A)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using MHC class 1 Antibody(AAA46900)HLA-A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HLA-A Antibody (AAA46900)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of GAPDH expression in(1) Hela cell lysate;(2)Jurkat cell lysate;(3)Mouse kidney lysate;(4) Mouse spleen lysate;(5) RAW 2647 cell lysate;(6) Rat brain lysate with GAPDH Antibody (AAA46909).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded(1) Rat colon;(2) Mouse testis;(3) Human uterus;(4) Human kidney, using GAPDH Antibody (AAA46909).)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells, using GAPDH Antibody)
WB (Western Blot) (Western blot analysis of MLKL expression in HUVEC cell lysate (AAA46955).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLKL)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using MLKL Antibody(AAA46955)MLKL was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MLKL Antibody (AAA46955)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of Desmin expression in (1) Mouse heart tissue lysate; (2) Rat heart tissue lysate (AAA47092).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DES monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DES)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody(AAA47092)DES was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DES Antibody (AAA47092)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human PD1 (A) were surface stained with anti-PD1 neutralizing antibody 1ug/ml (pembrolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.)
ELISA (Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human PD1 (A) were surface stained with anti-PD1 neutralizing antibody 1ug/ml (pembrolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Application Data (The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Anti-GAPDH monoclonal antibody (1:20,000)Lane 2.: Anti-GAPDH monoclonal antibody (1:10,000)Lane 3.: Anti-GAPDH monoclonal antibody (1:2,000)Lane 4.: Anti-GAPDH monoclonal antibody (1:200))
Application Data (The tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse Brain tissue lysateLane 2.: Mouse Eye tissue lysateLane 3.: Mouse Muscle tissue lysate)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: MCF7 cell lysateLane 3.: Jurkat cell lysateLane 4.: A549 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0592 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence) (ICC/IF analysis of EIF2S1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF2S1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: NIH-3T3 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of SAR1B in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SAR1B antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The extract of Mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SAR1B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.)
IF (Immunofluorescence) (ICC/IF analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB1 antibody (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : HeLa cell lysate Lane 3. : HepG2 cell lysate Lane 4. : 293T cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of ACAT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (Western blot analysis The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Ramos cell lysate Lane 3.: MCF-7 cell lysate Lane 4.: 293T cell lysate Lane 5.: HL-60 cell lysate Lane 6.: SW480 cell lysate The Recombinant Human ACAT2 (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: ACAT2 Transfected 293T cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of SOD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SOD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : A431 cell lysate Lane 3. : 293T cell lysate Lane 4. : PC3 cell lysate Lane 5. : U87MG cell lysate The recombinant protein (20ng) of SOD two isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 (1:1000). Lane 1 : Recombinant protein SOD1 Lane 2 : Recombinant protein SOD2)
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot) (The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of GMFB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GMFB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: GMFB transfected 293T cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
WB (Western Blot) (The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BUB1B protein in paraffin embedded Human endometrium tissue using BUB1B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BUB1B protein (Right) or empty vector (Left) detected with BUB1B antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of AKR1A1 protein in paraffin embedded Human liver tissue using AKR1A1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AKR1A1 protein (Right) or empty vector (Left) detected with AKR1A1 antibody)
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
AKR1A1 antibody was purified by affinity chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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