Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (KRT16/CK16/Cytokeratin 16 Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT16 (1:2000).)
Application Data (Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.)
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using Chromogranin A Recombinant Mouse Monoclonal Antibody (rCHGA/413) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis of Purified Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/413).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Pancreas stained with Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/413).)
WB (Western Blot) (RNA pol II phospho Ser2 antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with RNA pol II phospho Ser2 antibody (mAb) at a 1 ug/ml dilution.)
IF (Immunofluorescence) (Detection of RNA Pol II pS2 by immunofluorescence. U2OS cells were stained with RNA Pol II pS2 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: RNA Pol II pS2 antibody staining. Right panel: merge.)
ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq. ChIP-Seq was performed in a bladder cancer cell line using RNA pol II CTD phospho Ser5 antibody and RNA pol II CTD phospho Ser2 antibody . The average ChIP-Seq signal across all genes is shown in the graphic. As expected Pol II phosphoserine 5 is enriched at promoters and phosphoserine 2 is enriched toward the 3’ end of genes.)
ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2.3 million HL-60 cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify RNA pol II phospho Ser2 binding. Data is compared to ChIP-Seq data using a phospho Ser5 antibody (61085). ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser2 antibody detects polymerase more toward the 3´ end of the genes and the phospho Ser5 antibody detects Pol II more at the 5´ end of the genes.)
ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 10 ug of chromatin from human myeloma LP1 cells and 20 ug RNA pol II CTD phospho Ser2 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
WB (Western Blot) (RNA pol II antibody (mAb) (Clone 1F4B6) tested by Western blot. HeLa nuclear extract (30 ug per lane) probed with RNA pol II antibody (mAb) at a 1 ug/ml dilution.)
IF (Immunofluorescence) (RNA pol II antibody (mAb) (Clone 1F4B6) tested by immunofluorescence. Left: HeLa cell stained with RNA pol II antibody (mAb). Middle:Hoechst. Right: Merge.)
ChIP (Chromatin Immunoprecipitation) (RNA pol II antibody (mAb) (Clone 1F4B6) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HL-60 cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify RNA pol II binding sites. The image shows binding across a region of chromosome 15. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Application Data ((2ug/ml) staining in human peripheral blood monocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed Daudi cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)
Application Data ((0.001ug/ml) staining in A431(A), HeLa(B), MCF7(C), HepG2(D) and A549(E) cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila. Does not react with bacteria, helminths, and spinach
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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