Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.)
WB (Western Blot) (Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.)
WB (Western Blot) (Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 ug). Lane 3: Mouse 3T3NIH lysate (10 ug). Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Tissue: Corneal Endothelial Cell (CEC). Species: Rabbit. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Courtesy of: Eunduck E.P. Kay, Doheny Eye Institute.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.)
WB (Western Blot) (MCF7 cells were subjected to SDS PAGE followed by western blot with AAA102677 (ALDH1A1 antibody) at dilution of 1:500)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain tissue slide using AAA102677 (ALDH1A1 Antibody) at dilution of 1:200)
Application Data (Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody.)
Application Data (Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7&Rat testis cell lysates using RAD9A antibody (1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa.)
WB (Western Blot) (Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 10H7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 10H7. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RFXANK protein using RFXANK antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of RFXANK protein in paraffin embedded Carcinoma of Human lung tissue using RFXANK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RFXANK protein (Right) or empty vector (Left) detected with RFXANK antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSX1 protein using SSX1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSX1 protein (Right) or empty vector (Left) detected with SSX1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SSX1 protein (red) or empty vector (blue) stained using SSX1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SCYL3 protein (Right) or empty vector (Left) detected with SCYL3 antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of SCYL3 protein in paraffin embedded Adenocarcinoma of Human breast tissue using SCYL3 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSR1 protein using SSR1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of SSR1 protein in paraffin embedded Human pancreas tissue using SSR1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSR1 protein (Right) or empty vector (Left) detected with SSR1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant MGRN1 protein using MGRN1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MGRN1 protein (Right) or empty vector (Left) detected with MGRN1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant CXorf26 protein using CXorf26 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CXorf26 protein in paraffin embedded Human lymphoma tissue using CXorf26 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CXorf26 protein (Right) or empty vector (Left) detected with CXorf26 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant LIPG protein using LIPG antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of LIPG protein in paraffin embedded Carcinoma of Human liver tissue using LIPG antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LIPG protein (Right) or empty vector (Left) detected with LIPG antibody)
WB (Western Blot) (Figure-3: Western Blot analysis: The purified antibodies 3H4.1F7 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0ug/ml (lane 3), (4) RBD protein, (5) unrelated protein 1 ,(6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.)
WB (Western Blot) (Anti-Nucleocapsid monoclonal Antibodies (3H4.1F7) was screened against different proteins to evaluate the specificity of the antibodies.)
ELISA (Figure-2: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 3H4.1F7. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
WB (Western Blot) (Figure-1: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM3H4.1F7)) was used at 4ug/ml on (1) SARS-CoV-2 virus infected Vero Cell lysates and (2) Mock infected lysates.)
WB (Western Blot) (Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TUBB4 protein using TUBB4 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBB4 protein (Right) or empty vector (Left) detected with TUBB4 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using TUBB4 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RASD2 protein (Right) or empty vector (Left) detected with RASD2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RASD2 protein (red) or empty vector (blue) stained using RASD2 antibody)
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U251 cells using anti-IFI16 antibody (AAA126880).Overlay histogram showing U251 cells stained with AAA126880 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IFI16 Antibody (AAA126880, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 2. IF analysis of IFI16 using anti-IFI16 antibody (AAA126880).IFI16 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-IFI16 Antibody (AAA126880) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of IFI16 using anti-IFI16 antibody (AAA126880).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Daudi whole cell lysates,Lane 2: human Raji whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human U-87 MG whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IFI16 antigen affinity purified monoclonal antibody (#AAA126880) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFI16 at approximately 98 kDa. The expected band size for IFI16 is at 88 kDa.)
Application Data (Published researcher image:Mouse anti Rabbit CD8 antibody, clone 12.C7 (AAA50109) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.Image caption:Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry.A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis).B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P)
FITC (Fluorescein Isothiocyanate) (Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 (AAA50109) followed by Goat anti Mouse IgG (H/L): FITC)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse IgG1 isotope control . Figure B. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse anti Pig CD45RC . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD45RC . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD45RC detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
FCM/FACS (Flow Cytometry) (Staining of normal human peripheral blood platelets 0.06 ug of Purified anti-human CD61 (VI-PL2) followed by PE Donkey Anti-Mouse IgG (H+L). Samples were then extensively washed and co-stained with FITC anti-human CD42b (HIP1) to label platelets. CD42b(-) events represent cell debris.)
IHC (Immunohiostchemistry) (Anti-Integrin Beta 3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
IHC (Immunohistochemistry) (Anti-Integrin Beta 3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
FCM/FACS (Flow Cytometry) (Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.)
IHC (Immunohistochemistry) (Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
WB (Western Blot) (Jurkat extract probed with anti-c-myc monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence. Intact (non-degraded) c-myc with a molecular weight of approximately 62 kD was detected.)
IHC (Immunohiostchemistry) (Anti-MYC / c-Myc antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry) (Anti-MYC / c-Myc antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohiostchemistry) (Anti-S100A10 antibody IHC of human nerve and ganglion cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry) (Anti-S100A10 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
WB (Western Blot) (Western blot of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/ml.)
ICC (Immunocytochemistry) (Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/ml.)
IF (Immunofluorescence) (Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 2 ug/ml.)
IHC (Immunohiostchemistry) (Anti-XBP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry) (Anti-XBP1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
WB (Western Blot) (Extracts of Mouse brain (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red).)
IHC (Immunohistochemistry) (Anti-TUBB / Tubulin, Beta antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Application Data (Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human CD147 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Application Data (Staining of human erythrocytes with Mouse anti Human CD147)
Application Data (Staining of human erythrocytes with Mouse anti Human CD147:APC)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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