Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: HeLa cell lysateLane 3.: 293T cell lysate)

IF (Immunofluorescence) (ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with AAA48411 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

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PDCD4, Monoclonal Antibody (Cat# AAA48411)

Full Name

Human PDCD4 antibody

Gene Names

PDCD4; H731

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD81:Low Endotoxin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD81)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD81: FITC)

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CD81, Monoclonal Antibody (Cat# AAA49071)

Full Name

MOUSE ANTI HUMAN CD81:FITC

Gene Names

CD81; S5.7; CVID6; TAPA1; TSPAN28

Applications

Flow Cytometry

Pricing

$500/0.1 mg $975/2x0.1 mg $1,430/3x0.1 mg $1,855/4x0.1 mg $2,040/5x0.1 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human BLTR: Azide Free)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human BLTR)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:Alexa Fluor 647)

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BLTR, Monoclonal Antibody (Cat# AAA49086)

Full Name

MOUSE ANTI HUMAN BLTR:FITC

Gene Names

LTB4R; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1

Applications

Flow Cytometry, Immunohistochemistry

Pricing

$580/0.1 mg $1,120/2x0.1 mg $1,635/3x0.1 mg $2,125/4x0.1 mg $2,370/5x0.1 mg

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Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD8)

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CD8, Monoclonal Antibody (Cat# AAA49105)

Full Name

RAT ANTI HUMAN CD8:FITC

Gene Names

CD8A; CD8; MAL; p32; Leu2

Applications

Flow Cytometry

Pricing

$500/100 Tests $975/2x100 Tests $1,430/3x100 Tests $1,855/4x100 Tests $2,040/5x100 Tests

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Application Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD134:Alexa Fluor 647)

Application Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD134:FITC)

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CD134, Monoclonal Antibody (Cat# AAA49119)

Full Name

MOUSE ANTI RAT CD134:FITC

Gene Names

Tnfrsf4; Ox40; Txgp1

Applications

Flow Cytometry

Pricing

$525/0.1 mg $1,015/2x0.1 mg $1,485/3x0.1 mg $1,930/4x0.1 mg $2,140/5x0.1 mg

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IF (Immunofluorescence) (ICC/IF analysis of HSPA13 in Hep3B cells. The cell was stained with ATGA0477 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSPA13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate)

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HSPA13, Monoclonal Antibody (Cat# AAA48942)

Full Name

HSPA13 antibody

Gene Names

HSPA13; STCH

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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IF (Immunofluorescence) (ICC/IF analysis of HPRT in HaLe cells. The cell was stained with AAA48956 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate)

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HPRT, Monoclonal Antibody (Cat# AAA48956)

Full Name

HPRT antibody

Gene Names

HPRT1; HPRT; HGPRT

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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Application Data (Published customer image: Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)

Application Data (Rat Splenocytes stained with Mouse anti Rat OX-62:RPE)

Application Data (Published customer image: The expression of OX62+DCs and OX62+CD4+SIRP+DCs of each groups at various development stages (Mean +/- SD). (a) The expression level of OX62+DCs of each group at different stages. (b) The expression level of OX62+CD4+SIRP+ DCs of each group at different stages. (c) Single-cell suspensions of the total PP-DCs in rats were identified by OX62. The difference of OX62+DC among groups at different development stages was not significant. (F = 3.0, 0.587, 3.267, and 1.471, resp.; P >.05). Significant growth occurred in LR group for the number of OX62+CD4+SIRP+DCs at age week 3. Levels of OX62+CD4+SIRP+DC subsets at every age were highly significant in LR group and BR group compared with AR group. Furthermore, the positive cell numbers were higher in LR group than in BR group. The positive cell numbers kept stable in LR group at various ages while the positive cells number increased with age in the other two groups. Significant numbers of OX62+CD4+SIRP+DCs were found in LR and BR group at various ages (Figures 1(b) and 1(c)) compared with AR group (A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K: AR at W11, L: LR at W11). *P )

Application Data (Published customer image:Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: x200 (A, C, D) and x400 (B).From: Tiurbe G, Matuschek A, K¤mmerer U, Schneider M, Thiede A, Ulrichs K, Otto C. Inhibitory effects of rat bone marrow-derived dendritic cells on na¯ve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10. BMC Res Notes. 2009 Jan 23;2:12.)

Application Data (Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 ug/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.)

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OX-62, Monoclonal Antibody (Cat# AAA49229)

Full Name

MOUSE ANTI RAT OX-62

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,735/5x0.1 mg

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DB (Dot Blot) (Dot blot of 5-Hydroxymethylcytosine (5-hmC) mAb. Dot blot analysis was used to confirm the specificity of 5-Hydroxymethylcytosine antibody for 5-hydroxymethylcytidine. 10 ng of single-stranded 38 nt DNA oligonucleotides were spotted onto nitrocellulose and probed with the antibody at 0.2mg/mL. Lane 1: oligo containing unmodified cytidine. Lane 2: oligo containing 5-methylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-formylcytidine. Lane 5: oligo containing 5-carboxylcytidine.)

DB (Dot Blot) (5-Hydroxymethylcytosine (5-hmC) antibody (mAb) tested by dot blot analysis. DNA samples were spotted (indicated in ng on the left) on to a positively charged nylon membrane and blotted with 5-Hydroxymethylcytidine antibody at a 0.2 mg/ml dilution. Lane 1: double-stranded DNA containing 5-hydroxymethylcytosine. Lane 2: double-stranded DNA containing 5-methylcytosine. Lane 3: unmethylated double-stranded DNA. Lane 4: single-stranded DNA containing 5-hydroxymethylcytosine. Lane 5: single-stranded DNA containing 5-methylcytosine. Lane 6: unmethylated single-stranded DNA.)

IP (Immunoprecipitation) (5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 2 ?g of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 2 ?g of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydroxymethylcytosine methylation (5-hmC).)

ChIP (Chromatin Immunoprecipitation) (hMeDIP-chip performed on human brain DNA using 5-Hydroxymethylcytosine (5-hmC) antibody. Human brain DNA (2 ug) was immunoprecipitated with 10 ug of 5-Hydroxymethylcytosine antibody. Following hMeDIP, the DNA was amplified, labeled and hybridized to an Affymetrix Human Tiling 2.0R Array. Shown is a region from chromosome 6q containing the ARID1B, ZDHHC14 and SNX9 genes. The results show that 5-hydroxymethylcytosine is enriched primarily in the coding regions of genes, rather than in the gene promoter or regulatory regions.)

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5-Hydroxymethylcytosine, Monoclonal Antibody (Cat# AAA59960)

Full Name

5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Dot Blot

Purity

Protein G Chromatography

Pricing

$240/0.01 mg $410/0.05 mg $665/0.1 mg $1,295/2x0.1 mg $1,890/3x0.1 mg $2,450/4x0.1 mg $2,815/5x0.1 mg

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DB (Dot Blot) (Histone H3 acetyl Lys56 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys56 peptide. Lane 2: unmodified H3 peptide. Lane 3: acetyl-Lys4 peptide. Lane 4: acetyl-Lys9 peptide. Lane 5: acetyl-Lys14 peptide. Lane 6: acetyl-Lys18 peptide. Lane 7: acetyl-Lys23 peptide. Lane 8: acetyl-Lys27 peptide. Lane 9: acetyl-Lys36 peptide. Lane 10: acetyl-Lys64 peptide.)

ChIP (Chromatin Immunoprecipitation) (Chromatin IP analysis of Histone H3 acetyl Lys56 antibody. Chromatin IP was performed using Low Cell ChiP Kit using 3 ug of Histone H3 acetyl Lys56 mAb. RT-qPCR was performed on ChIP DNA using a primer pair specific for the PABPC1 gene or a negative control region. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

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Histone H3K56ac, Monoclonal Antibody (Cat# AAA59970)

Full Name

Histone H3K56ac antibody (mAb)(Clone 12.1)

Reactivity

Budding Yeast, Fission Yeast, Human, Wide Range Predicted

Applications

Dot Blot, Chromatin immunoprecipitation

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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Application Data ((1ug/ml) staining in Molt4 cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of Jurkat cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fixPLUS cover-slips. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)

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CD8, Monoclonal Antibody (Cat# AAA59553)

Full Name

CD8 Monoclonal Antibody [BU88]

Gene Names

CD8A; CD8; MAL; p32; Leu2

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein A purified

Pricing

$420/0.02 mg $560/0.05 mg $650/0.1 mg $1,260/2x0.1 mg $1,845/3x0.1 mg $2,390/4x0.1 mg $2,640/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence-Anti-HEF1 Antibody [2G9])

IHC (Immunohistochemisry) (Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-HEF1 antibody [2G9] normal breast tissue and ductal carcinoma)

Application Data ((0.1ug/ml) staining in MCF7 cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor 488 secondary antibody.)

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HEF1/NEDD-9, Monoclonal Antibody (Cat# AAA59565)

Full Name

HEF1/NEDD-9 Monoclonal Antibody [2G9]

Gene Names

NEDD9; CAS2; CASL; HEF1; CAS-L; CASS2

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein G

Pricing

$915/0.05 mg $1,780/2x0.05 mg $2,600/3x0.05 mg $3,375/4x0.05 mg $3,825/5x0.05 mg

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ChIP (Chromatin Immunoprecipitation) (MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human lung melanoma cell line MALME-3M and 4 ug of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

ChIP (Chromatin Immunoprecipitation) (MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.)

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MITF, Monoclonal Antibody (Cat# AAA59930)

Full Name

MITF antibody (mAb)(Clone C5.D5)

Reactivity

Human

Applications

Western Blot, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Purity

Protein G Chromatography

Pricing

$250/0.01 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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WB (Western Blot) (Histone macroH2A1.2 antibody (mAb) tested by Western blot. U2OS cells mock transfected (lane 1) or transfected with tagged macroH2A1.1 (lane 2), macroH2A1.2 (lane 3) or macroH2A2 (lane 4) were probed with Histone macroH2A1.2 antibody at a dilution of 1:300. Band at 40kD is the endogenous macroH2A1.2.)

IF (Immunofluorescence) (Histone macroH2A1.2 antibody (mAb) tested by immunofluorescence. Staining of macroH2A1.2 transfected HeLa cells with Histone macroH2A1.2 antibody (1:50 dilution, left panel) and DAPI (right panel).)

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Histone macroH2A1.2, Monoclonal Antibody (Cat# AAA60026)

Full Name

Histone macroH2A1.2 antibody (mAb)(Clone 14G7-H9)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Ascites

Pricing

$295/0.01 mL $740/0.1 mL $1,445/2x0.1 mL $2,110/3x0.1 mL $2,735/4x0.1 mL $3,090/5x0.1 mL

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IF (Immunofluorescence) (ICC/IF analysis of PEBP1 in HeLa cells line, monoclonal anti-human PEBP1 antibody (1:100) stained with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : SKOV3 cell lysate Lane 3 : A549 cell lysate Lane 4 : HepG2 cell lysate Lane 5 : MCF7 cell lysate Lane 6 : K562 cell lysate Lane 7 : LnCap cell lysate Lane 8 : Ramos cell lysate)

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PEBP1, Monoclonal Antibody (Cat# AAA48269)

Full Name

PEBP1 antibody

Gene Names

PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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WB (Western Blot) (The mouse tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse brain tissue lysate)

ICC (Immunocytochemistry) (ICC/IF analysis of Rab5a in U87MG cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

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Rab5a, Monoclonal Antibody (Cat# AAA48282)

Full Name

Rab5a

Gene Names

RAB5A; RAB5

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein-A Affinity Chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

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WB (Western Blot) (The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cystatin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: U87MG cell lysate)

ICC (Immunocytochemistry) (ICC/IF analysis of Cystatin B in A549 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

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Cystatin B, Monoclonal Antibody (Cat# AAA48291)

Full Name

Cystatin B

Gene Names

CSTB; PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)

WB (Western Blot) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: U87MG cell lysate)

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MEMO1, Monoclonal Antibody (Cat# AAA48338)

Full Name

MEMO1

Gene Names

MEMO1; MEMO; C2orf4; CGI-27; NS5ATP7

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

Application Data

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Quinidine, Monoclonal Antibody (Cat# AAA47714)

Full Name

Quinidine Monoclonal Antibody

Applications

ELISA

Pricing

$340/0.1 mg $590/0.5 mg $1,065/1 mg $2,065/2x1 mg $3,015/3x1 mg $3,915/4x1 mg $4,610/5x1 mg

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IF (Immunofluorescence) (ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (Lane 2.: NFATc1 transfected 293T cell lysate)

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RBP4, Monoclonal Antibody (Cat# AAA48046)

Full Name

RBP4 antibody

Gene Names

RBP4; RDCCAS; MCOPCB10

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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IF (Immunofluorescence) (ICC/IF analysis of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

IHC (Immunohiostchemistry) (Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : K562 cell lysate Lane 3. : Jurkat cell lysate Lane 4. : MCF-7 cell lysate)

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FkBP4, Monoclonal Antibody (Cat# AAA48049)

Full Name

FkBP4 antibody

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

WB (Western Blot) (Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

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HCN4, Monoclonal Antibody (Cat# AAA103372)

Full Name

HCN4 Antibody: ATTO 390

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,355/5x0.1 mg

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Application Data (Figure A. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse IgG1 isotope control . Figure B. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse anti Pig CD45RC . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD45RC . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD45RC detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)

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CD45RC, Monoclonal Antibody (Cat# AAA50364)

Full Name

Mouse Anti Pig CD45RC

Reactivity

Pig

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

$450/0.1 mg $880/2x0.1 mg $1,285/3x0.1 mg $1,660/4x0.1 mg $1,800/5x0.1 mg

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Application Data (Surface Staining of human peripheral blood lymphocytes with Mouse anti Human TCR gamma/delta:RPE)

Application Data (Surface Staining of human peripheral blood lymphocytes with Mouse anti Human TCR gamma/delta:FITC)

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TCR Gamma/Delta, Monoclonal Antibody (Cat# AAA50392)

Full Name

Mouse Anti Human TCR Gamma/Delta

Gene Names

CD3E; T3E; TCRE; IMD18

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,865/5x0.1 mg

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Application Data (Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human CD147 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

Application Data (Staining of human erythrocytes with Mouse anti Human CD147)

Application Data (Staining of human erythrocytes with Mouse anti Human CD147:APC)

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CD147, Monoclonal Antibody (Cat# AAA49485)

Full Name

MOUSE ANTI HUMAN CD147:RPE

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Applications

Flow Cytometry

Pricing

$600/100 Tests $1,160/2x100 Tests $1,695/3x100 Tests $2,200/4x100 Tests $2,465/5x100 Tests

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: endotoxin low)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2)

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HLA A2, Monoclonal Antibody (Cat# AAA49489)

Full Name

MOUSE ANTI HUMAN HLA A2:RPE

Gene Names

HLA-A; HLAA

Applications

Flow Cytometry

Pricing

$660/100 Tests $1,285/2x100 Tests $1,875/3x100 Tests $2,430/4x100 Tests $2,745/5x100 Tests

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Application Data (Staining of rat spleen cells with Mouse anti Rat CD45RA:Alexa Fluor 488)

Application Data (Staining of rat spleen cells with Mouse anti Rat CD45RA: FITC)

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CD45RA, Monoclonal Antibody (Cat# AAA49399)

Full Name

MOUSE ANTI RAT CD45RA (B CELLS ONLY)

Gene Names

Ptprc; Lca; RT7; CD45; L-CA; T200

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$605/0.25 mg $1,170/2x0.25 mg $1,710/3x0.25 mg $2,220/4x0.25 mg $2,475/5x0.25 mg

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Application Data (Staining of rat spleen cells with Mouse anti Rat CD44)

Application Data (Staining of rat spleen cells with Mouse anti Rat CD44: FITC)

Application Data (Staining of rat spleen cells with Mouse anti Rat CD44:FITC)

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CD44, Monoclonal Antibody (Cat# AAA49428)

Full Name

MOUSE ANTI RAT CD44

Gene Names

Cd44; CD44A; METAA; RHAMM

Applications

Flow Cytometry, Immunohistochemistry

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,735/5x0.1 mg

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Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD59)

Application Data (Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59)

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CD59, Monoclonal Antibody (Cat# AAA49434)

Full Name

RAT ANTI HUMAN CD59

Gene Names

CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Functional Assay, Flow Cytometry, Immunohistochemistry

Pricing

$575/0.2 mg $1,110/2x0.2 mg $1,620/3x0.2 mg $2,105/4x0.2 mg $2,360/5x0.2 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64)

Application Data (Staining of peripheral blood monocytes with Mouse anti Human CD64)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:Alexa Fluor 647)

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CD64, Monoclonal Antibody (Cat# AAA49441)

Full Name

MOUSE ANTI HUMAN CD64

Gene Names

FCGR1A; CD64; FCRI; CD64A; IGFR1

Applications

Flow Cytometry

Pricing

$575/0.2 mg $1,110/2x0.2 mg $1,620/3x0.2 mg $2,105/4x0.2 mg $2,360/5x0.2 mg

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Application Data (Published customer image: Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)

Application Data (Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor 647)

Application Data (Published customer image: Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)

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CD4, Monoclonal Antibody (Cat# AAA49647)

Full Name

MOUSE ANTI SHEEP CD4:RPE

Applications

Flow Cytometry

Pricing

$785/100 Tests $1,525/2x100 Tests $2,230/3x100 Tests $2,895/4x100 Tests $3,295/5x100 Tests

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Application Data (Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:RPE)

Application Data (Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:FITC)

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MHC CLASS II DQ DR POLYMORPHIC, Monoclonal Antibody (Cat# AAA49650)

Full Name

MOUSE ANTI SHEEP MHC CLASS II DQ DR POLYMORPHIC:RPE

Applications

Flow Cytometry

Pricing

$785/100 Tests $1,525/2x100 Tests $2,230/3x100 Tests $2,895/4x100 Tests $3,295/5x100 Tests

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64)

Application Data (Staining of peripheral blood monocytes with Mouse anti Human CD64)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD64:Alexa Fluor 647)

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CD64, Monoclonal Antibody (Cat# AAA49705)

Full Name

MOUSE ANTI HUMAN CD64

Gene Names

FCGR1A; CD64; FCRI; CD64A; IGFR1

Applications

Flow Cytometry

Pricing

$245/0.025 mg $480/2x0.025 mg $700/3x0.025 mg $895/5x0.025 mg $910/4x0.025 mg

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Application Data (Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human cytokeratin 14 folllowing heat mediated antigen retrieval using citrate buffer, pH6.0)

Application Data (Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (low power) following heat mediated antigen retrieval using citrate buffer at pH6.2)

Application Data (Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (high power) following heat mediated antigen retrieval using citrate buffer at pH6.2)

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CYTOKERATIN 14, Monoclonal Antibody (Cat# AAA49717)

Full Name

MOUSE ANTI HUMAN CYTOKERATIN 14

Gene Names

KRT14; K14; NFJ; CK14; EBS3; EBS4

Applications

Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunohistochemistry

Pricing

$250/0.02 mg $490/2x0.02 mg $715/3x0.02 mg $920/5x0.02 mg $930/4x0.02 mg

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Application Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag)

Application Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP)

Application Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody)

Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 . Visualised using a LI-COR Odyssey infrared imaging system)

Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP)

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HISTIDINE TAG, Monoclonal Antibody (Cat# AAA49724)

Full Name

MOUSE ANTI HISTIDINE TAG

Applications

Western Blot, Immunoprecipitation

Pricing

$1,610/2 mg $3,130/2x2 mg $4,570/3x2 mg $5,935/4x2 mg $6,995/5x2 mg

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Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD98)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD98)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD98: Alexa Fluor 647)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD98: Alexa Fluor 488)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD98:FITC)

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CD98, Monoclonal Antibody (Cat# AAA49733)

Full Name

RAT ANTI MOUSE CD98

Gene Names

Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110

Applications

Flow Cytometry

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human Macrophages:RPE . Permeabilised with Leucoperm)

Application Data (Macrophages stained with Mouse anti Human Macrophages/ Monocytes using enhanced DAB in experimental allergic marmoset brain. Mag. X 400)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages. Permeabilised with Leucoperm)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC . Permeabilised with Leucoperm)

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MACROPHAGES/MONOCYTES, Monoclonal Antibody (Cat# AAA49741)

Full Name

MOUSE ANTI HUMAN MACROPHAGES:Low Endotoxin

Gene Names

S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$890/0.5 mg $1,730/2x0.5 mg $2,525/3x0.5 mg $3,275/4x0.5 mg $3,765/5x0.5 mg

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Application Data (Staining of dog peripheral lymphocytes with Rat anti Dog CD5:APC)

Application Data (Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor647)

Application Data (Staining of dog peripheral lymphocytes with Rat anti Dog CD5 followed by Goat F(ab')2 anti Rat IgG:FITC)

Application Data (Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor488)

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CD5, Monoclonal Antibody (Cat# AAA49754)

Full Name

RAT ANTI DOG CD5:APC

Applications

Flow Cytometry

Pricing

$765/100 Tests $1,495/2x100 Tests $2,185/3x100 Tests $2,835/4x100 Tests $3,225/5x100 Tests

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Application Data (Published customer imageCharacterization of pSer40/41MCM2 antibody. A) HeLa whole cell extract was incubated with Lambda phosphatase in the presence or absence of phosphatase inhibitors. Proteins were analyzed by western blot with the indicated antibodies. B) Equal amounts of protein extract were separated on a single SDS-PAGE gel and transferred onto membranes. Vertical slices of membrane (each with identical protein content) were then incubated with anti-pSer40/41MCM2 antibody in the presence of the indicated competitor peptides (upper panels). Membranes were then re-probed with an anti-MCM2 antibody (lower panels). Identical exposures are shown. C) HeLa cells growing on coverslips were fixed and stained with anti-pSer40/41MCM2 antibody in the presence of the indicated competitor peptides. DNA was counterstained with DAPI. D) Serial sections of normal and cancerous colon tissue were used in IHC with the pSer40/41MCM2 antibody in the presence or absence of competitor peptide. Nuclear positivity is shown by brown color. E) HeLa cells were treated with PHA-767491 for the indicated time. Protein extracts were prepared and analysed by western blot with the indicated antibodies.From: FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, et al. (2014) A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE 9(6): e98891.)

Application Data (Paraffin embedded human tonsil stained with Mouse anti Human MCM2 following citrate antigen retrieval)

Application Data (Published customer image: Ryuvidine and Mitoxantrone reduce CDC7 levels and pSer40/41 MCM2 phosphorylation. A) Molecular structure of PHA-767491, Ryuvidine and Mitoxantrone. B) HeLa cells were incubated with either CDC7 inhibitor PHA-767491, Ryuvidine or Mitoxantrone at 10 micromolar each for the indicated time. Protein extracts were prepared and analyzed by western blot with the indicated antibodies. C) Levels of Cdc7-independent pSer41MCM2 and Cdc7-dependent pSer40/41MCM2 phosphorylation assessed in HeLa cells treated with the indicated concentration of PHA-767491, Ryuvidine or Mitoxantrone. D) Cells were incubated with either PHA-767491, Ryuvidine or Mitoxantrone at the indicated concentration for nine hours. Protein extracts were then prepared and analyzed by western blot with the indicated antibodies. E) HeLa cells incubated with either PHA-767491, Ryuvidine or Mitoxantrone at 10 micromolar each for nine hours in the presence or absence of 20 micromolar of caspase inhibitor Boc-D-fmk. Levels of Ser40/41MCM2 phosphorylation, CDC7 and MCM2 abundance as well as PARP cleavage, were assessed by western blotting.From: FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, et al. (2014) A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE 9(6): e98891.)

Application Data (Published customer image: Confirmation and in vitro characterization of Hit compounds. A) HeLa cells were treated with the ten compounds that produced the strongest reduction in pSer40/41MCM2 levels in the primary screen. Protein extracts were prepared and levels of residual pSer40/41MCM2 phosphorylation were measured by western blot analysis with the indicated antibodies. B) The same compounds were tested for their ability to inhibit CDC7 kinase activity in in vitro kinase assay. Kinase reactions were performed on a synthetic MCM2 substrate in the absence or presence of the indicated drug and reactions were run on SDS-PAGE gels. CDC7 activity on the synthetic MCM2 substrate was monitored by Western blotting with an anti-pSer108MCM2 antibody. As a control the levels of CDC7 kinase and synthetic MCM2 substrate present in each reaction were also assessed and shown to be similar in all reactions.From: FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, et al. (2014) A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE 9(6): e98891.)

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MCM2, Monoclonal Antibody (Cat# AAA49305)

Full Name

MOUSE ANTI HUMAN MCM2

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,735/5x0.1 mg

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Application Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD16/32: Biotin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD16/32: FITC)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD16/32 : RPE)

Application Data (Staining of mouse peripheral blood lymphocytes Rat anti Mouse CD16/32: Alexa Fluor 488)

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CD16/CD32, Monoclonal Antibody (Cat# AAA49360)

Full Name

RAT ANTI MOUSE CD16/CD32

Gene Names

Fcgr2b; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik

Applications

Flow Cytometry

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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Application Data (Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21)

Application Data (Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:Alexa Fluor 647)

Application Data (Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:RPE)

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CD21, Monoclonal Antibody (Cat# AAA49611)

Full Name

MOUSE ANTI CANINE CD21:RPE

Reactivity

Horse, Cat, Racoon
N.B. Antibody reactivity and working conditions vary between species

Applications

Flow Cytometry

Pricing

$855/100 Tests $1,660/2x100 Tests $2,420/3x100 Tests $3,145/4x100 Tests $3,605/5x100 Tests

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Application Data (Staining of human bone marrow. Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm)

Application Data (Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human MYELOPEROXIDASE:FITC)

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MYELOPEROXIDASE, Monoclonal Antibody (Cat# AAA49293)

Full Name

MOUSE ANTI HUMAN MYELOPEROXIDASE

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$575/0.2 mg $1,110/2x0.2 mg $1,620/3x0.2 mg $2,105/4x0.2 mg $2,360/5x0.2 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.