Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ERK1/2 Mouse mAb diluted at 1:200.)

WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate. 2)Mouse Brain Tissue Lysate. 3) Rat Brain Tissue Lysate using ERK1/2 Mouse mAb diluted at 1:2000.)

Compare (5 max)

ERK 1/2, Monoclonal Antibody (Cat# AAA320522)

Full Name

ERK 1/2 (PTR1327) Mouse Monoclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Protein G

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of Rat-brain tissue. 1.Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-appendix tissue. 1.Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.Peroxiredoxin 1 Monoclonal Antibody(8E7) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

WB (Western Blot) (Western blot analysis of 1) MCF7. 2) Rat Kidney Tissue. 3) Mouse Brain Tissue. diluted at 1:2000.)

Compare (5 max)

Peroxiredoxin 1, Monoclonal Antibody (Cat# AAA320441)

Full Name

Peroxiredoxin 1 Monoclonal Antibody (8E7)

Gene Names

PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver in 30ug/ml dilute concentrations.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using AAA118844 in 30ug/ml dilute concentrations.)

Compare (5 max)

C-reactive protein, Monoclonal Antibody (Cat# AAA118844)

Full Name

Mouse anti-Human C-reactive protein(CRP) Monoclonal Antibody

Gene Names

CRP; PTX1

Reactivity

Human. Other species are not tested. Please decide the specificity by homology

Applications

Immunohistochemistry

Purity

>95%, Protein G Purified

Pricing

$280/0.05 mg $375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,680/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red). Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green). Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).)

WB (Western Blot) (The Cell lysate (40ug) and Mouse tissue extracts (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: A549 cell lysate Lane 3.: A431 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: Mouse Brain tissue extract)

Compare (5 max)

beta-Tubulin, Monoclonal Antibody (Cat# AAA48084)

Full Name

beta-Tubulin antibody

Gene Names

TUBB2B; CDCBM7; PMGYSA; bA506K6.1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of EEF1A1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EEF1A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EEF1A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate Lane 3.: Raji cell lysate Lane 4.: THP-1 cell lysate Lane 5.: MCF-7 cell lysate)

Compare (5 max)

EEF1A1, Monoclonal Antibody (Cat# AAA48187)

Full Name

EEF1A1 antibody

Gene Names

EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GRP94 Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: HepG2 cell lysate)

Compare (5 max)

GRP94, Monoclonal Antibody (Cat# AAA48190)

Full Name

GRP94 antibody

Gene Names

HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SET7/9 transfected 293T cell lysate)

Compare (5 max)

SET 7/9, Monoclonal Antibody (Cat# AAA47978)

Full Name

SET 7/9 antibody

Gene Names

SETD7; KMT7; SET7; SET9; SET7/9

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

WB (Western Blot) (The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EphA2 extracellular domain transfected 293T cell lysate Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate)

Compare (5 max)

EphA2, Monoclonal Antibody (Cat# AAA48031)

Full Name

EphA2 antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

WB (Western Blot) (Western blot analysis of G-CSF expression in K562 cell lysate (AAA47106).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CSF3)

Compare (5 max)

G-CSF, Monoclonal Antibody (Cat# AAA47106)

Full Name

Anti-G-CSF Rabbit Monoclonal Antibody

Gene Names

CSF3; GCSF; CSF3OS; C17orf33

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of BAD expression in Hela cell lysate (AAA47134).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAD)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Bad Antibody(AAA47134)BAD was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BAD Antibody (AAA47134)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

Bad, Monoclonal Antibody (Cat# AAA47134)

Full Name

Anti-Bad Rabbit Monoclonal Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

Application Data (Lane 1: mw markers; Lane 2: recombinant human ADA; Lane 3: calf spleen ADA)

Application Data

Compare (5 max)

Adenosine deaminase, Monoclonal Antibody (Cat# AAA47860)

Full Name

Adenosine deaminase Monoclonal Antibodies

Applications

Immunoblot

Pricing

$505/0.1 mg $985/2x0.1 mg $1,440/3x0.1 mg $1,875/4x0.1 mg $2,140/5x0.1 mg

Application Data

Compare (5 max)

Quinidine, Monoclonal Antibody (Cat# AAA47714)

Full Name

Quinidine Monoclonal Antibody

Applications

ELISA

Pricing

$340/0.1 mg $590/0.5 mg $1,065/1 mg $2,065/2x1 mg $3,015/3x1 mg $3,915/4x1 mg $4,610/5x1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (Lane 2.: NFATc1 transfected 293T cell lysate)

Compare (5 max)

RBP4, Monoclonal Antibody (Cat# AAA48046)

Full Name

RBP4 antibody

Gene Names

RBP4; RDCCAS; MCOPCB10

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

IHC (Immunohiostchemistry) (Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : K562 cell lysate Lane 3. : Jurkat cell lysate Lane 4. : MCF-7 cell lysate)

Compare (5 max)

FkBP4, Monoclonal Antibody (Cat# AAA48049)

Full Name

FkBP4 antibody

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PYCRL protein using PYCRL antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of PYCRL protein in paraffin embedded Carcinoma of Human kidney tissue using PYCRL antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PYCRL protein (Right) or empty vector (Left) detected with PYCRL antibody)

Compare (5 max)

PYCRL, Monoclonal Antibody (Cat# AAA108098)

Full Name

PYCRL antibody

Gene Names

PYCRL; PYCR3

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

PYCRL antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of PEBP1 in HeLa cells line, monoclonal anti-human PEBP1 antibody (1:100) stained with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : SKOV3 cell lysate Lane 3 : A549 cell lysate Lane 4 : HepG2 cell lysate Lane 5 : MCF7 cell lysate Lane 6 : K562 cell lysate Lane 7 : LnCap cell lysate Lane 8 : Ramos cell lysate)

Compare (5 max)

PEBP1, Monoclonal Antibody (Cat# AAA48269)

Full Name

PEBP1 antibody

Gene Names

PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (The mouse tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse brain tissue lysate)

ICC (Immunocytochemistry) (ICC/IF analysis of Rab5a in U87MG cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Compare (5 max)

Rab5a, Monoclonal Antibody (Cat# AAA48282)

Full Name

Rab5a

Gene Names

RAB5A; RAB5

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein-A Affinity Chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cystatin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: U87MG cell lysate)

ICC (Immunocytochemistry) (ICC/IF analysis of Cystatin B in A549 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Compare (5 max)

Cystatin B, Monoclonal Antibody (Cat# AAA48291)

Full Name

Cystatin B

Gene Names

CSTB; PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Vimentin expression in HEK293 cell lysate (AAA46913).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VIM)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody(AAA46913)VIM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VIM Antibody (AAA46913)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

Vimentin, Monoclonal Antibody (Cat# AAA46913)

Full Name

Anti-Vimentin Rabbit Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Survivin Antibody(AAA46939)Birc5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Birc5 Antibody (AAA46939)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Western blot analysis of Survivin expression in Neuro-2a cell lysate (AAA46939).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Birc5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Birc5)

Compare (5 max)

Survivin, Monoclonal Antibody (Cat# AAA46939)

Full Name

Anti-Survivin Rabbit Monoclonal Antibody

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Cytokeratin 5 expression in A431 cell lysate (AAA46942).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT5)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human urithelial carcinoma, using Cytokeratin 5 (C-term) Antibody(AAA46942)KRT5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT5 Antibody (AAA46942)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

Cytokeratin 5, Monoclonal Antibody (Cat# AAA46942)

Full Name

Anti-Cytokeratin 5 (C-term) Rabbit Monoclonal Antibody

Gene Names

KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)

WB (Western Blot) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: U87MG cell lysate)

Compare (5 max)

MEMO1, Monoclonal Antibody (Cat# AAA48338)

Full Name

MEMO1

Gene Names

MEMO1; MEMO; C2orf4; CGI-27; NS5ATP7

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$310/0.05 mL $395/0.1 mL $765/2x0.1 mL $1,090/5x0.05 mL $1,120/3x0.1 mL $1,450/4x0.1 mL $1,480/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of PKC alpha expression in HEK293 cell lysate (AAA46984).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKCA)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody(AAA46984)PRKCA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKCA Antibody (AAA46984)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

PKC alpha, Monoclonal Antibody (Cat# AAA46984)

Full Name

Anti-PKC alpha Rabbit Monoclonal Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of using Integrin beta1 expression in U937 cell lysate (AAA46991).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGB1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human bladder carcinoma, using Integrin beta 1 Antibody(AAA46991)ITGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGB1 Antibody (AAA46991)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

Integrin beta 1, Monoclonal Antibody (Cat# AAA46991)

Full Name

Anti-Integrin beta 1 Rabbit Monoclonal Antibody

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

WB (Western Blot) (Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Compare (5 max)

HCN4, Monoclonal Antibody (Cat# AAA103372)

Full Name

HCN4 Antibody: ATTO 390

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,355/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of HSPA13 in Hep3B cells. The cell was stained with ATGA0477 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSPA13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate)

Compare (5 max)

HSPA13, Monoclonal Antibody (Cat# AAA48942)

Full Name

HSPA13 antibody

Gene Names

HSPA13; STCH

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (ICC/IF analysis of HPRT in HaLe cells. The cell was stained with AAA48956 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate)

Compare (5 max)

HPRT, Monoclonal Antibody (Cat# AAA48956)

Full Name

HPRT antibody

Gene Names

HPRT1; HPRT; HGPRT

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

Application Data (Published customer image: Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)

Application Data (Rat Splenocytes stained with Mouse anti Rat OX-62:RPE)

Application Data (Published customer image: The expression of OX62+DCs and OX62+CD4+SIRP+DCs of each groups at various development stages (Mean +/- SD). (a) The expression level of OX62+DCs of each group at different stages. (b) The expression level of OX62+CD4+SIRP+ DCs of each group at different stages. (c) Single-cell suspensions of the total PP-DCs in rats were identified by OX62. The difference of OX62+DC among groups at different development stages was not significant. (F = 3.0, 0.587, 3.267, and 1.471, resp.; P >.05). Significant growth occurred in LR group for the number of OX62+CD4+SIRP+DCs at age week 3. Levels of OX62+CD4+SIRP+DC subsets at every age were highly significant in LR group and BR group compared with AR group. Furthermore, the positive cell numbers were higher in LR group than in BR group. The positive cell numbers kept stable in LR group at various ages while the positive cells number increased with age in the other two groups. Significant numbers of OX62+CD4+SIRP+DCs were found in LR and BR group at various ages (Figures 1(b) and 1(c)) compared with AR group (A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K: AR at W11, L: LR at W11). *P )

Application Data (Published customer image:Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: x200 (A, C, D) and x400 (B).From: Tiurbe G, Matuschek A, K¤mmerer U, Schneider M, Thiede A, Ulrichs K, Otto C. Inhibitory effects of rat bone marrow-derived dendritic cells on na¯ve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10. BMC Res Notes. 2009 Jan 23;2:12.)

Application Data (Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 ug/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.)

Compare (5 max)

OX-62, Monoclonal Antibody (Cat# AAA49229)

Full Name

MOUSE ANTI RAT OX-62

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

$435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,605/4x0.1 mg $1,735/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBAL3 protein (Right) or empty vector (Left) detected with TUBAL3 antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant TUBAL3 protein (red) or empty vector (blue) stained using TUBAL3 antibody)

Compare (5 max)

TUBAL3, Monoclonal Antibody (Cat# AAA107465)

Full Name

TUBAL3 antibody

Applications

Western Blot, Flow Cytometry

Purity

TUBAL3 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LMAN1 protein (Right) or empty vector (Left) detected with LMAN1 antibody)

Compare (5 max)

LMAN1, Monoclonal Antibody (Cat# AAA107547)

Full Name

LMAN1 antibody

Gene Names

LMAN1; MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53

Applications

Western Blot, Immunofluorescence

Purity

LMAN1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

ELISA (Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (AAA40982) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.)

ELISA (Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1ug/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; AAA40982 binds spike proteins of delta and omicron variants.)

ELISA (Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982. A direct ELISA was performed using SARS-CoV-2 Spike trimer recombinant proteins of WT, Omicron and Delta variants as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (AAA40982) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.4 ng/mL to 900 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982 can detect spike trimer protein at 1.5 ng/mL.)

Compare (5 max)

SARS-CoV-2 (COVID-19) S1 RBD, Monoclonal Recombinant Antibody (Cat# AAA40982)

Full Name

SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]

Reactivity

Virus

Applications

ELISA

Purity

SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.

Pricing

$630/0.1 mg $1,220/2x0.1 mg $1,780/3x0.1 mg $2,315/4x0.1 mg $2,590/5x0.1 mg

< >

<< Previous Next >>

ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6329, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6329, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)

Compare (5 max)

HIV-1 p24, Monoclonal Antibody (Cat# AAA40990)

Full Name

HIV-1 p24 Antibody [2F11G1](biotin)

Reactivity

Virus

Applications

Western Blot

Purity

Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

< >

<< Previous Next >>

ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA40992, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA40992, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)

Compare (5 max)

HIV-1 p24, Monoclonal Antibody (Cat# AAA40992)

Full Name

HIV-1 p24 Antibody [2F1A8]

Reactivity

Virus

Applications

Western Blot

Purity

Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

< >

<< Previous Next >>

ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6331, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6331, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)

Compare (5 max)

HIV-1 p24, Monoclonal Antibody (Cat# AAA40994)

Full Name

HIV-1 p24 Antibody [2F1A8](HRP)

Reactivity

Virus

Applications

Western Blot

Purity

Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot)

SDS-PAGE

Compare (5 max)

COVID 19 Spike (S1)-1 Coronavirus, Monoclonal Antibody (Cat# AAA41062)

Full Name

Anti-SARS-CoV-2 (S1)-1 Neutralizing antibody

Reactivity

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), SARS-COV

Applications

Western Blot

Purity

Affinity-chromatography

Pricing

$340/0.05 mg $435/0.1 mg $850/2x0.1 mg $1,235/3x0.1 mg $1,525/5x0.1 mg $1,605/4x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: HeLa cell lysateLane 3.: 293T cell lysate)

IF (Immunofluorescence) (ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with AAA48411 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Compare (5 max)

PDCD4, Monoclonal Antibody (Cat# AAA48411)

Full Name

Human PDCD4 antibody

Gene Names

PDCD4; H731

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

< >

<< Previous Next >>

Application Data (Figure 2: Western blot on a lysate of Cadherin 11 transfected PC3 cells)

Application Data (Figure 1: Immunocytochemistry on Cadherin 11 transfected PC3 cells)

Compare (5 max)

OB-Cadherin / Cadherin-11, Monoclonal Antibody (Cat# AAA77469)

Full Name

Mouse anti OB-Cadherin / Cadherin-11

Gene Names

CDH11; OB; CAD11; CDHOB; OSF-4

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoblot

Pricing

$720/0.1 mg $1,405/2x0.1 mg $2,055/3x0.1 mg $2,660/4x0.1 mg $3,030/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TTC32 protein (Right) or empty vector (Left) detected with TTC32 antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant TTC32 protein (red) or empty vector (blue) stained using TTC32 antibody)

Compare (5 max)

TTC32, Monoclonal Antibody (Cat# AAA107075)

Full Name

TTC32 antibody

Applications

Western Blot, Flow Cytometry

Purity

TTC32 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TUBAL3 protein using TUBAL3 antibody)

IHC (Immunohistochemisry) (Immunohistochemical analysis of TUBAL3 protein in paraffin embedded Human Kidney tissue using TUBAL3 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBAL3 protein (Right) or empty vector (Left) detected with TUBAL3 antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant TUBAL3 protein (red) or empty vector (blue) stained using TUBAL3 antibody)

Compare (5 max)

TUBAL3, Monoclonal Antibody (Cat# AAA107011)

Full Name

TUBAL3 antibody

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

TUBAL3 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK6 protein using NEK6 antibody)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of NEK6 protein in paraffin embedded Adenocarcinoma of Human colon tissue using NEK6 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK6 protein (Right) or empty vector (Left) detected with NEK6 antibody)

Compare (5 max)

NEK6, Monoclonal Antibody (Cat# AAA107054)

Full Name

NEK6 antibody

Gene Names

NEK6; SID6-1512

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

NEK6 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TRIM2 protein (Right) or empty vector (Left) detected with TRIM2 antibody)

FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using TRIM2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Compare (5 max)

TRIM2, Monoclonal Antibody (Cat# AAA106736)

Full Name

TRIM2 antibody

Gene Names

TRIM2; CMT2R; RNF86

Applications

Western Blot, Flow Cytometry

Purity

TRIM2 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant KATNB1 protein using KATNB1 antibody)

WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant KATNB1 protein (Right) or empty vector (Left) detected with KATNB1 antibody)

Compare (5 max)

KATNB1, Monoclonal Antibody (Cat# AAA106896)

Full Name

KATNB1 antibody

Gene Names

KATNB1; KAT; LIS6

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

KATNB1 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of beta III Tubulin expression in HeLa cell lysate (AAA47084).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBB3)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using beta III Tubulin Antibody(AAA47084)TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (AAA47084)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Compare (5 max)

beta III Tubulin, Monoclonal Antibody (Cat# AAA47084)

Full Name

Anti-beta III Tubulin Rabbit Monoclonal Antibody

Gene Names

TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human CD34 protein, His tagged protein PME100469 can bind Rabbit anti-CD34 monoclonal antibody (clone: DM135) in a linear range of 0. 1-12 ng/ml.)

ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human CD34 protein, His tagged protein PME100469 can bind Rabbit anti-CD34 monoclonal antibody (clone: DM135) in a linear range of 0. 1-12 ng/ml.)

Compare (5 max)

CD34, Monoclonal Antibody (Cat# AAA47457)

Full Name

Anti-CD34 antibody (DM135), Rabbit mAb

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$315/0.01 mg $750/0.1 mg $1,930/0.5 mg $3,755/2x0.5 mg $5,490/3x0.5 mg $7,125/4x0.5 mg $8,360/5x0.5 mg

FCM/FACS (Flow Cytometry) (Figure 1. 1e5 of Expi 293 cell line were stained with 100 uL of 1:1000 diluted Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb (Blue histogram) or isotype control (Red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)

Compare (5 max)

B7-H3, Monoclonal Antibody (Cat# AAA47459)

Full Name

Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$365/0.01 mg $1,055/0.1 mg $2,785/0.5 mg $5,435/2x0.5 mg $7,940/3x0.5 mg $10,305/4x0.5 mg $12,305/5x0.5 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.