Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ICC (Immunocytochemistry) (ICC staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Versican antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Versican antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with Noxa antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noxa antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Noxa antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CDT1 on Hela cell using anti-CDT1 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-DAZL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testes tissue using anti-DAZL antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with PMP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining PMP22 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PMP22 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with PGC1 beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
WB (Western Blot) (Western blot analysis of PGC1 beta on rat spleen tissue lysate using anti-PGC1 beta antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NIH-3T3 cells with Galectin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Galectin 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Galectin 1 on HL-60 cell (1) and human skeletal muscle tissue (2) lysate using anti-Galectin 1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SW480 cells with JAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-JAK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-JAK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: PC-12 Lane 2: Jurkat Lane 3: NIH-3T3)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with TIMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FAAH1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-FAAH1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-FAAH1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FAAH1 on different lysates using anti-FAAH1 antibody at 1/2, 000 dilution. Positive control: Lane 1: SK-Br-3 Lane 2: Mouse cerebellum Lane 3: Mouse lung)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3 (mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H3 (mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 (mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining Histone H3 (mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Histone H3 (mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Histone H3 (mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Application Data (Figure 3. Affinity ranking of different Rabbit anti-CD38 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD38 monoclonal antibody (clone: DM30) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Application Data
Application Data (Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD38(right) were surface stained with Rabbit anti-CD38 monoclonal antibody 1μg/ml (clone: DM30) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Factor XIIIa in paraffin-embedded Human colon cancer tissue using Factor XIIIa Rabbit mAb at dilution 1/100)
WB (Western Blot) (Western blot detection of Factor XIIIa in Jurkat cell lysates using Factor XIIIa Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:83kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of PARK7/DJ1 in paraffin-embedded Human tonsil using PARK7/DJ1 Rabbit mAb at dilution 1/100)
IF (Immunofluorescence) (Immunofluorescence of PARK7/DJ1 (green) in Hela using PARK7/DJ1 antibody at dilution 1/2, and DAPI(blue))
WB (Western Blot) (Western blot detection of PARK7/DJ1 in K562, Rat Brain,C6,3T3,Hela cell lysates using PARK7/DJ1 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:22kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of CD8 in paraffin-embedded Human tonsil using CD8 Rabbit mAb at dilution 1/1)
WB (Western Blot) (Western blot detection of CD8 in K562,CHO-K1,Hela cell lysates using CD8 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:29KDa.)
WB (Western Blot) (Western blot detection of CD8 in THP-1 cell lysates using CD8 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:29KDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Caveolin-1 in paraffin-embedded Human breast cancer tissue using Caveolin-1 Rabbit mAb at dilution 1/20)
WB (Western Blot) (Western blot detection of Caveolin-1 in Hela cell lysates using Caveolin-1 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:20kDa.)
WB (Western Blot) (Detection of mouse PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 ug) from ND7/23, C6, RAW 264.7, J774A1, and AML12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (AAA213685 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (10 ug) from MCF-10A, SNB-75, HaCaT, MCF-7, and RT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (AAA213685 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human CD86 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HDLM-2, Jurkat, MJ, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (AAA213539 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human CD86 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD86 recombinant monoclonal antibody (AAA213539 lot 4) used for IP at 6 ul per reaction. CD86 was also immunoprecipitated by a previous lot of this antibody (AAA213539 lot 1) and a second antibody against a different epitope of CD86 . For blotting immunoprecipitated CD86, AAA213539 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemisry) (Detection of CD86 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (AAA213539 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG ,)
ICC (Immunocytochemistry) (Detection of CD86 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (AAA213539 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG ,)
FCM/FACS (Flow Cytometry) (Detection of human CD86 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-CD86 recombinant monoclonal (AAA213539 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEPA1 Mouse Monoclonal Antibody (PMEPA1/2697).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with PMEPA1 Mouse Monoclonal Antibody (PMEPA1/2697).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human pancreas carcinoma (A). esophagus carcinoma tissue (B) and ovary tumor tissue (C). showing cytoplasmic and membrane localization with DAB staining using 4E-BP1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using 4E-BP1 Monoclonal Antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).)
IF (Immunofluorescence) (Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using AATM Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot) (Western Blot analysis using AATM Monoclonal Antibody against HEK293 (1). PC-12 (2). HL-60 (3). BCBL-1 (4). HepG2 (5) and NIH/3T3 (6) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using AIF-M1 Monoclonal Antibody (blue) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using AIF-M1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer tissues with DAB staining using AIF-M1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using AIF-M1 Monoclonal Antibody against NIH/3T3 (1). Jurkat (2). HeLa (3). HepG2 (4). MOLT4 (5). C6 (6). RAJI (7). Cos7 (8) and PC-12 (9) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human Alzheimer brain tissue. showing cytoplasmic localization with DAB staining using APP Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using APP Monoclonal Antibody against truncated APP recombinant protein.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using Axl Monoclonal Antibody (green). showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot) (Western Blot analysis using Axl Monoclonal Antibody against extracellular domain of human AXL (aa19-444).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using Bcl-10 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of Hela cells using Bcl-10 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B). showing cytoplasmic localization with DAB staining using Bcl-10 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Bcl-10 Monoclonal Antibody against NIH/3T3 (1). HeLa (2). MCF-7 (3) and Jurkat (4) cell lysate.)
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.