Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Staining of thrombin activated platelets with Mouse anti Human CD62P)
Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free)
Application Data (Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC (AAA50118))
Application Data (Staining of human peripheral blood platelets with Mouse anti Human CD62P)
Application Data (Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin))
Application Data (Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Application Data (Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Application Data (Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. Pacific Blue conjugated Mouse anti Human CD14 and RPE conjugated Mouse IgG2b isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD14 and RPE conjugated Mouse anti Human CD68 . All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 and FITC conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 and FITC conjugated Mouse anti Human CD68 . All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. PE conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse IgG2b isotype control . Figure B. PE conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse anti Human CD68 . All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD3 . Permeabilised with Leucoperm)
Application Data (Formalin fixed, paraffin embedded human tonsil stained with Rat anti Human CD3 following heat mediated antigen retrieval in EDTA buffer pH9.0)
Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)
Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD3 following permeabilisation with Leucoperm)
Application Data (Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Mouse anti Human CD3 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
ELISA (Sandwich ELISA analysis of human haptoglobin using Mouse anti Human haptoglobin (AAA50426) as a capture reagent and biotinylated Mouse anti Human haptoglobin as a detection reagent with purified native human haptoglobin as the antigen. Detecti)
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
ELISA (Sandwich ELISA analysis of human tPA using Mouse anti Human tPA (AAA50435) as a capture reagent and biotinylated Mouse anti Human tPA as a detection reagent with purified native tPA as the antigen. Detection was performed using HRP conjugated)
Application Data (Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD21 (AAA50482). All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Mouse anti Human CD21 antibody, clone Bu33 used to stain formalin fixed, paraffin embedded human spleen sections by immunohistochemistry, visualized by biotinylated anti-Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).)
WB (Western Blot) (Western strip blots of HeLa cell crude extracts with two different preparations of CD107a / LAMP1 antibody in strips 17 and 18. Both preparations bind to a diffuse band running at between ~90kDa and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.)
ICC (Immunocytochemistry) (HeLa cells staining with CD107a / LAMP1 antibody (green), and counterstained with chicken polyclonal antibody to Lamin A/C (red) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.)
IHC (Immunohiostchemistry) (Anti-LAMP1 / CD107a antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
IHC (Immunohistochemistry) (Anti-LAMP1 / CD107a antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IP (Immunoprecipitation) (Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.)
WB (Western Blot) (Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.)
IHC (Immunohistochemistry) (Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)
WB (Western Blot) (Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
IF (Immunofluorescence) (Anti-CD163 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.)
IHC (Immunohistochemistry) (Anti-CD163 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA3.)
IHC (Immunohistochemistry) (Anti-MAGEA3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
FCM/FACS (Flow Cytometry) (Flow cytometry of Jurkat cells, using anti-ALDH1L1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
WB (Western Blot) (Western blot of extracts (35 ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1.)
WB (Western Blot) (Western strip blots of HeLa cell crude extracts stained with anti LAMP1 antibody CD107a / LAMP1 antibody in strip 9. Lane 10 shows staining another LAMP1 antibody. Both antibodies bind to a diffuse band running at between ~90 and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.)
ICC (Immunocytochemistry) (HeLa cells staining with CD107a / LAMP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong punctate cytoplasmic staining corresponding to lysosomes and late endosomes, while the Vimentin antibody reveals cytoplasmic intermediate filaments.)
IHC (Immunohiostchemistry) (Anti-LAMP1 / CD107a antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohistochemistry) (Anti-LAMP1 / CD107a antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Protein A purified and supplied in 0.01 M PBS without preservative. Purity >95% by HPLC & SDS-PAGE
Pricing
LF (Lateral Flow) (Lateral Flow Application for Nucleocapsid Antigen Detection:- Conjugate pad: Colloidal gold conjugated (SARS-CoV-2 Nucleocapsid mAb with Clone # 9B3) & Colloidal gold (Chicken IgY).- T line: AAA59156 (SARS-CoV-2 Nucleocapsid mAb with Clone # 2B3).- C line: (Rabbit anti-chicken IgY).The recombinant SARS-CoV-2 Nucleocapsid full-length protein was used as Positive Control, at the original concentration 0.44 mg/ml (lot specific). The minimum detection limit for was 22 pg/ml. Lane 1: 1x PBS was added at the sample hole (Negative); Lane 2: with dilution at 1:1000 (Positive); Lane 3: with dilution at 1:10,000 (Positive); Lane 4: with dilution at 1:100,000 (Positive); Lane 5: with dilution at 1:1,000,000 (Positive); Lane 6: with dilution at 1:10,000,000 (Positive); Lane 7: with dilution at 1:20,000,000 (Positive).)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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