Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
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IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with MALT1 Monoclonal Antibody (SPM578))
SDS-PAGE (SDS-PAGE Analysis Purified CD28 Mouse Monoclonal Antibody (CB28). Confirmation of Integrity and Purity of Antibody.)
FCM/FACS (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)
IF (Immunofluorescence) (Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to label nuclei (red).)
SDS-PAGE (SDS-PAGE Analysis of Purified Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1773R).)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Parathyroid stained with Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1773R).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Pancreas stained with Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1773R).)
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using AIF1 Mouse Monoclonal Antibody (rAIF/1909) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis of Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373) (unconjugated). Confirmation of Integrity and Purity of Antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometry for human ER-alpha on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).)
WB (Western Blot) (Western Blot Analysis of Human Stomach, Mouse Stomach and Rat Stomach tissue lysates using ALDH1A1 Mouse Monoclonal Antibody (ALDH1A1/1381).)
WB (Western Blot) (Western Blot Analysis of Human Brain tissue lysate using ALDH1A1 Mouse Monoclonal Antibody (ALDH1A1/1381).)
SDS-PAGE (SDS-PAGE Analysis of Purified ALDH1A1 Mouse Monoclonal Antibody (ALDH1A1/1381). Confirmation of Integrity and Purity of Antibody.)
WB (Western Blot) (Western Blot Analysis of human Liver tissue lysate using ALDH1A1 Mouse Monoclonal Antibody (ALDH1A1/1381).)
WB (Western Blot) (Western Blot of K562 and Lung Lysate using ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).)
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using MART-1 (MLANA) Rabbit Monoclonal Antibody (MLANA/1761R)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IF (Immunofluorescence) (Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S Recombinant Rabbit Anti-GFAP (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).)
FCM/FACS (Flow Cytometry) (Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling RM46 with M25212 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (Rm46 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25212 of 10 ug. Western blot was performed from the immunoprecipitate using M25212 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : RM46 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 32 KDa Observed band size : 32 KDa Blocking/Dilution buffer : 1x TBST.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PABP2 with M25211 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (PABP2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25211 of 10 ug. Western blot was performed from the immunoprecipitate using M25211 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : PABP2 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 33 KDa Observed band size : 50 KDa Blocking/Dilution buffer : 1x TBST.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling K2C6B with M25207 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (K2C6B was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25207 of 10 ug. Western blot was performed from the immunoprecipitate using M25207 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : K2C6B Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MIMIT with M25103 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (MIMIT was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25103 of 10 ug. Western blot was performed from the immunopre-cipitate using M25103 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 20 KDaObserved band size : 20 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling VIME with M25003 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (VIME was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25003 of 10 ug. Western blot was performed from the immunoprecipitate using M25003 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2:IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : VIME Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% Skim milk conjugated at 1/10000 dilution Predicted band size : 54 KDa Observed band size : 54 KDa Blocking/Dilution buffer : 1x PBS.)
IHC (Immunohistochemistry) (Ck18 was immunoprecipitated from 1mg of PC9 cells membrane fraction, blotted with M26000 of 10 ug. Western blot was performed from the immunopre-cipitate using M26000 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: PC9 cells membrane fraction. Lane2: IP product of PC9 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
Application Data ((H) The arthritis severity was evaluated by the arthritis scores. (I) Paw swelling was measured every 5 days after anti-CD109 addition. (J) Paw photographs from mice with CIA captured on day 21 after starting anti-CD109 treatment.)
Application Data (Aneutralizing antibody against CD109 (anti-CD109) were designed for functional or treatment studies in RA FLSs and CIA.The migration (K) and invasion (L) of RA FLSs from six different patients (three males and three females) were assessed and quantified (graph below) after the addition of anti-CD109 (10 ug/mL) or IgG (10 ug/mL) and incubation for 24 hours. ***p)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling HSP7C with AAA62312 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (HSP7C was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with AAA62312 of 10 ug. Western blot was performed from the immunopre-cipitate using AAA62312 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 71 KDaObserved band size : 71 KDaBlocking and dilution buffer and concentration: 5% milk/TBST.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling G3BP1 with M25069 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (G3BP1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25069 of 10 ug. Western blot was performed from the immunopre-cipitate using M25069 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 52 KDaObserved band size : 52 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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