Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohistochemisry) (Recommended Immunohistochemical ProtocolPretreatment1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.2. Air dry for 2 hours at 58° C.3. Oeparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: a. Electric Pressure CookerPlace standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides toroom temperature.b. Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the Immuno DNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C.lncubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. Wash slides with IHC wash buffer or 01 water.8. Continue IHC staining protocol. Immunohistochemical Protocol)
Staining Procedure (1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.b. TintoRetriever PT Module or Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.8. Wash slides with IHC wash buffer or DI water.9. Continue IHC staining protocol.)
Application Data (Presentation)
IHC (Immunohistochemistry) (Inset: IHC of NeuN on a FFPE Brain Tissue)
Protein A purified and supplied in 0.01 M PBS without preservative. Purity >95% by HPLC & SDS-PAGE
Pricing
LF (Lateral Flow) (Lateral Flow Application for Nucleocapsid Antigen Detection:- Conjugate pad: Colloidal gold conjugated (SARS-CoV-2 Nucleocapsid mAb with Clone # 9B3) & Colloidal gold (Chicken IgY).- T line: AAA59156 (SARS-CoV-2 Nucleocapsid mAb with Clone # 2B3).- C line: (Rabbit anti-chicken IgY).The recombinant SARS-CoV-2 Nucleocapsid full-length protein was used as Positive Control, at the original concentration 0.44 mg/ml (lot specific). The minimum detection limit for was 22 pg/ml. Lane 1: 1x PBS was added at the sample hole (Negative); Lane 2: with dilution at 1:1000 (Positive); Lane 3: with dilution at 1:10,000 (Positive); Lane 4: with dilution at 1:100,000 (Positive); Lane 5: with dilution at 1:1,000,000 (Positive); Lane 6: with dilution at 1:10,000,000 (Positive); Lane 7: with dilution at 1:20,000,000 (Positive).)
Application Data (SDS-PAGE analysis of GR-3G7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified GR- 3G7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GR-3G7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data (Fig.2: BOSC23 cells were transiently transfected with expression vectors containing the cDNA of the light chain of botulinum toxin A-F. Expression of the constructs was tested with an anti-myc antibody (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GR-3G7 was tested on all botulinum toxin transfectants. A positive signal was obtained only with BoNT/B transfected cells (red curves).)
Application Data (Fig.1: FACS analysis of BOSC23 cells using GR-3G7 Cat.# GM-0701. BOSC23 cells were transiently transfected with an expression vector encoding either BoNT/B (red curve) or an irrelevant protein (control transfectant). Binding of GR-3G7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with BoNT/B transfected cells.)
Application Data (SDS-PAGE analysis of HT-12D8 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data (Fig3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: ?-myc; CEACAM20: ?-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HT-12D8 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).)
Application Data (Fig.2: Spectral Confocal Microscopy of CHO cells using HT-12D8 Cat.# GM-0516. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data (Fig.1: FACS analysis of BOSC23 cells using HT-12D8 Cat.# GM-0516. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM20 (red curve) or an irrelevant protein (control transfectant). Binding of HT-12D8 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells.)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5ug/mL.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1ug/mL.)
ELISA (Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD and S1 Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibody, AAA40981. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087) as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike RBD antibody (AAA40981) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike RBD Antibody, AAA40981 can detect spike RBD protein at 10 ng/mL and can weakly detect spike S1 protein at 200 ng/mL.)
ELISA (Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1ug/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and AAA40981 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.)
SARS-CoV-2 (COVID-19) Spike RBD Single Domain Antibody [T5P8-F9]
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P8-F9] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP6V1B1 protein using ATP6V1B1 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ATP6V1B1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using ATP6V1B1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP6V1B1 protein (Right) or empty vector (Left) detected with ATP6V1B1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BTN1A1 protein using BTN1A1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BTN1A1 protein (Right) or empty vector (Left) detected with BTN1A1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant DTYMK protein using DTYMK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DTYMK protein (Right) or empty vector (Left) detected with DTYMK antibody)
IF (Immunofluorescence) (Immunofluorescencestainingoffixedmousesplenocyteswithanti-ICOS(inducibleT-cellcostimulator)antibody7E.17G9(AAA72495) Immunofluorescenceanalysisofparaformaldehydefixedrat(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%Tritonstainedwiththechimericrabbitversionof7E.17G9(AAA72495)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72495,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
Application Data (Bindingcurveofanti-ICOSantibody7E.17G9(AAA72495)tomouseICOS-Fcfusionprotein ELISAPlatecoatedwithmouseICOS-Fcfusionprotein(Pr00153-1.9)ataconcentrationof5ug/ml.A3-foldserialdilutionfrom10,000to0.1ng/mlwasperformedusingAAA72495-23.0.Fordetection,a1:4000dilutionofHRP-labelledgoatanti-rabbitantibody(Bio-Rad)wasused.)
IF (Immunofluorescence) (Immunofluorescencestainingoffixedmousesplenocyteswithanti-ICOS(inducibleT-cellcostimulator)antibody7E.17G9(AAA72495) Immunofluorescenceanalysisofparaformaldehydefixedrat(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%Tritonstainedwiththechimericrabbitversionof7E.17G9(AAA72495)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72495,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SOX12 mAb. [Lot No. 86C2a-1])
WB (Western Blot) (Detection of human SOX12 by Western blot.Samples: Whole cell lysate (25 ug) from HT-1080 cells. [Lot No. 86C2a-1]Predicted molecular weight: 34 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-SOX12 monoclonal antibody.)
IF (Immunofluorescence) (ImmunofluorescencestainingoffixedRAW264.7cellswithanti-CD32antibodyAT130-5(AAA72431) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofAT130-5(AAA72431)at10?g/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2?g/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb01055-23.0*,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.*Ab01055isaduplicateofAAA72431;theysharethesamesequence.)
IF (Immunofluorescence) (Immunofluorescencestainingoffixedmousesplenocyteswithanti-CD32antibodyAT130-5(AAA72431) Immunofluorescenceanalysisofparaformaldehydefixedmouse(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofAT130-5(AAA72431)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembranestaininginasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72431,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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