Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ELISA (Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).)
ELISA (Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.)
WB (Western Blot) (Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.)
WB (Western Blot) (Western blot analysis of (1) 20 ng of cMyc-tagged recombinant GGP1 protein and (2) 20 ng of cMyc-tagged recombinant GGP1 protein using 1 μg of cMyc-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.)
WB (Western Blot) (Western blot analysis of (1) 200ng, (2) 100 ng, (3) 50 ng, and (4) 25 ng of a cMyc-tag-containing recombinant GGP1 protein with cMyc-tag antibody at 0.5 μg/ml.)
IHC (Immunohistochemisry) (Immunohistochemistry of TMIGD2 in human colon carcinoma tissue with TMIGD2 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of TMIGD2 in human colon carcinoma tissue with TMIGD2 antibody at 20 μg/mL.)
ICC (Immunocytochemistry) (Immunocytochemistry of TMIGD2 in transfected HEK293 cells with TMIGD2 antibody at 2 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 2 μg/mL.)
WB (Western Blot) (Figure 2 WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK).Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by WB.)
ELISA (Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA.)
IHC (Immunohistochemistry) (Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (AAA40961) at 20 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.)
ELISA (Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide, but not WT peptide and Alpha variant spike S1 peptide)
ELISA (Figure 2 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.)
Application Data (Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISACoating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10ug/ml.)
IF (Immunofluorescence) (Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10ug/ml.Green: PD-L1 Antibody [F2G2] (AAA40971)Blue: DAPI staining)
PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5ug/mL.)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1ug/mL.)
ELISA (Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA40989, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA40989, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFS1 with M25022 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.All lanes : NDUFS1 Mouse mAb at 1/2000 dilutionLane 1 : Jurkat cells membrane fractionLane 2 : THP1 cells membrane fractionLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 79 KDaObserved band size :79 KDaBlocking/Dilution buffer : 1x TBST.)
IP (Immunoprecipitation) (NDUFS1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25022 of 10 ug. Western blot was performed from the immunopre-cipitate using M25022 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
IF (Immunofluorescence) (ImmunofluorescencestainingofHepG2cellswithanti-PAR2antibody1B5(AAA72545). Immunofluorescenceanalysisofparaformaldehyde-fixedHepG2cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionof1B5(AAA72545)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72545,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-CD81 antibody 1.3.3.22 (AAA72168). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody or the rabbit IgG version of 1.3.3.22 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of fixed Daudi cells with anti-CD81 antibody 1.3.3.22 (AAA72168) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (Western Blot using anti-MHC I antibody R1-21.2 Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of R1-21.2 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
IF (Immunofluorescence) (Immunofluorescence staining of mouse splenocytes using anti-MHC I antibody R1-21.2. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of R1-21.2 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using Chromogranin A Recombinant Mouse Monoclonal Antibody (rCHGA/413) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis of Purified Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/413).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Pancreas stained with Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/413).)
IHC (Immunohistochemistry) (Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD71 (AAA71365) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Application Data (CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
FCM/FACS (Flow Cytometry) (Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
WB (Western Blot) (Western blot analysis of CD11b expression in TF1 cell lysate (AAA46885).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGAM)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(AAA46885)ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (AAA46885)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody (AAA47052)KIT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KIT Antibody (AAA47052)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of c-Kit expression in Human fetal lung tissue lysate (AAA47052).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIT)
WB (Western Blot) (Western blot analysis of MAP1LC3A expression in Hela cell lysate (AAA47065).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP1LC3A)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody(AAA47065)MAP1LC3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA47065)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
ELISA (Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L and TMB substrate were used for detection.)
DB (Dot Blot) (Anti-Insulin Antibody - Dot Blot. Dot blotting. Mab anti-Insulin antibody (clone 2D11. H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 ul of non-denatured human insulin at various dilutions starting at 1.0 ug/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (reacted for 45 min at room temperature and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
IHC (Immunohistochemistry) (Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
WB (Western Blot) (Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.)
IHC (Immunohiostchemistry) (Anti-WDHD1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
IHC (Immunohistochemistry) (Anti-WDHD1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
WB (Western Blot) (Western blot of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using antibody at a dilution of 3 ug/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.)
FCM/FACS (Flow Cytometry) (Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR9 antibody.)
IHC (Immunohistochemistry) (Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
WB (Western Blot) (Western blot of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein (100 ng/lane) using at 0.2 ug/ml.)
FCM/FACS (Flow Cytometry) (Intracellular flow cytometry of IL-17A in human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, using at 0.1 ug/10^6 cells (green represents isotype control, red represents IL-17A antibody). Goat anti-mouse IgG-FITC secondary antibody, was used for this test.)
IHC (Immunohistochemistry) (Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml.)
FCM/FACS (Flow Cytometry) (Flow cytometry of MHC Class II antibody)
IHC (Immunohistochemistry) (Anti-MHC Class II antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Application Data ((2ug/ml) staining in human peripheral blood monocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed Daudi cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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