Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A purification
Pricing
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta tissue slide using LIFR Monoclonal Antibody at dilution of 1:60.Positive staining was localized to trophoblast.)
FCM/FACS (Flow Cytometry) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD28 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(left). Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(Alexa Fluor 488 Conjuaged)(right) are used as control.)
FCM/FACS (Flow Cytometry) (C57BL/6 murine bone marrow cells are stained with Anti-Mouse Ly6C Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained bone marrow cells (empty black histogram) are used as control.)
Application Data (CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
FCM/FACS (Flow Cytometry) (Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
ELISA (Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).)
ELISA (Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.)
WB (Western Blot) (Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.)
IF (Immunofluorescence) (ICC/IF analysis of CRADD in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRADD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of CRADD in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRADD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (Fig.1: The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig2: The Recombinant Human CRADD (50,100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of PGAM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Jurkat cell lysate Lane 3. : Raji cell lysate Lane 4. : A431 cell lysate Lane 5. : HeLa cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of CDKN1b in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDKN1b antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDKN1b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : MCF-7 cell lysate Lane 2 : NIH/3T3 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein)
IF (Immunofluorescence) (ICC/IF analysis of human HeLa cells stained with Hoechst 3342 (Blue) for chromatin staining and monoclonal anti-human Aurora kinase B antibody (1:2000) with Texas Red (Red).)
WB (Western Blot) (The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Aurora kinase B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Aurora kinase A Lane 2.: Recombinant Human Aurora kinase B)
WB (Western Blot) (The lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OAT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse liver tissue lysate Lane 2.: 293T cell lysate)
ICC (Immunocytochemistry) (ICC/IF analysis of OAT in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence) (ICC/IF analysis of FADD in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FADD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry) (Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Raw264.7 cell lysate Lane 3. : MCF7 cell lysate Lane 4. : A431 cell lysate Lane 5. : Ramos cell lysate Lane 6. : Raji cell lysate Lane 7. : Balb/3T3 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of PPM1G in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
IHC (Immunohiostchemistry) (Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (The 293T cell lysate (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot) (Western blot analysis of CD11b expression in TF1 cell lysate (AAA46885).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGAM)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(AAA46885)ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (AAA46885)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
WB (Western Blot) (Western blot analysis of IRF5 expression in THP-1 cell lysate (AAA47011).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody (AAA47052)KIT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KIT Antibody (AAA47052)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of c-Kit expression in Human fetal lung tissue lysate (AAA47052).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIT)
WB (Western Blot) (Western blot analysis of MAP1LC3A expression in Hela cell lysate (AAA47065).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP1LC3A)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody(AAA47065)MAP1LC3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA47065)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Affinity-chromatography
Pricing
IHC (Immunohistochemistry) (Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD71 (AAA71365) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-CD81 antibody 1.3.3.22 (AAA72168). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody or the rabbit IgG version of 1.3.3.22 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of fixed Daudi cells with anti-CD81 antibody 1.3.3.22 (AAA72168) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Purified antibody. Protein A affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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