Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)
Application Data (Published customer image: Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?+/-?0.21% (n?=?3). Average CD45+: 0.19?+/-?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:Alexa Fluor488)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:FITC)
Application Data (Published customer image: Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?+/-?0.21% (n?=?3). Average CD45+: 0.19?+/-?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.)
Application Data (Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
Application Data (Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
Application Data (Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
FCM/FACS (Flow Cytometry) (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:FITC and Mouse anti Pig wCD8a:RPE)
FCM/FACS (Flow Cytometry) (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:APC and Mouse anti Pig wCD8a:RPE)
Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:RPE and Mouse anti Pig wCD8a:FITC)
Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27 detected with Goat anti Mouse IgG:FITC , and Mouse anti Pig wCD8a:RPE)
WB (Western Blot) (Western blot of anti-Thyroglobulin antibody at a dilution of 1:400. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 mg, right lane 3.0 mg) as confirmed by the position of molecular weight markers (not shown).)
WB (Western Blot) (Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
IHC (Immunohistochemistry) (Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
WB (Western Blot) (Western blot of TLR4 using antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.)
FCM/FACS (Flow Cytometry) (Flow analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of antibody. Shaded histogram represents cells without anti-TLR4 antibody; green represents isotype control (BD Pharmingen); red represents anti-TLR4 antibody.)
IHC (Immunohistochemistry) (Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
MONOTOPE purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
IF (Immunofluorescence) (Immunofluorescencestainingoffixedmousesplenocyteswithanti-ICOS(inducibleT-cellcostimulator)antibody7E.17G9(AAA72496) Immunofluorescenceanalysisofparaformaldehydefixedrat(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%Tritonstainedwiththechimericrabbitversionof7E.17G9(AAA72496)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72496,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
Application Data (Bindingcurveofanti-ICOSantibody7E.17G9(AAA72496)tomouseICOS-Fcfusionprotein ELISAPlatecoatedwithmouseICOS-Fcfusionprotein(Pr00153-1.9)ataconcentrationof5ug/ml.A3-foldserialdilutionfrom10,000to0.1ng/mlwasperformedusingAAA72496-23.0.Fordetection,a1:4000dilutionofHRP-labelledgoatanti-rabbitantibody(Bio-Rad)wasused.)
IF (Immunofluorescence) (Immunofluorescencestainingoffixedmousesplenocyteswithanti-ICOS(inducibleT-cellcostimulator)antibody7E.17G9(AAA72496) Immunofluorescenceanalysisofparaformaldehydefixedrat(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%Tritonstainedwiththechimericrabbitversionof7E.17G9(AAA72496)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72496,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IHC (Immunohistochemistry) (Paraformaldehyde-fixed, human colon cancer cells (LS174T) stained with c-erbB2 antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of cultured cells.)
IHC (Immunohiostchemistry) (CL7790AP (4ug/ml) staining of a human cerebral cortex formalin-fixed, paraffin-embedded tissue section; seen at 20x (left) and 40x (right) magnification. Strong staining of myelin observed in neuronal axons and dendrites.)
WB (Western Blot) (CL7790AP (0.3ug/ml) staining of a human brain (cerebral cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Application Data (Fig. 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2))
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MYBL2 mAb. [Lot No. MYBAD10A-2])
WB (Western Blot) (Detection of MYBL2 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. MYBAD10A-2]Predicted molecular weight: 78 kDa)
Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-MYBL2 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TCERG1 mAb. [Lot No. TCEAD79A-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TCERG1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. TCEAD79A-2])
WB (Western Blot) (Detection of TCERG1 by Western blot.Samples: Whole cell lysate from human A2058 (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. TCEAD79A-2]Predicted molecular weight: 123 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-TCERG1 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ETV3 mAb. [Lot No. ETV3F4D10-1])
WB (Western Blot) (Detection of human ETV3 by Western blot.Samples: Whole cell lysate (50 ug) from HeLa cells. [Lot No. ETV3F4D10-1]Predicted molecular weight: 16 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ETV3 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ATF3 mAb.)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ATF3 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SOX4 mAb. [Lot No.154C4a-2])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-SOX4 monoclonal antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DNMT3B mAb. [Lot No. 2280C3a-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DNMT3B mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2280C3a-1])
WB (Western Blot) (Detection of DNMT3B by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2280C3a-1]Predicted molecular weight: 95 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-DNMT3B monoclonal antibody.)
Application Data (Seasonal influenza A N1 antibody specifically recognizes seasonal (H1N1) N1, and does not cross-react with peptide corresponding to swine-origin influenza A (S-OIV, H1N1) N1 peptide, in ELISA.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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