Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).)
IP (Immunoprecipitation) (Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
WB (Western Blot) (Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.)
WB (Western Blot) (Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 ug). Lane 3: Acrolein-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.)
WB (Western Blot) (Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Application Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag)
Application Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP)
Application Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 . Visualised using a LI-COR Odyssey infrared imaging system)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP)
IHC (Immunohistochemisry) (Immunohistochemical analysis of SSB protein in paraffin embedded Carcinoma of Human thyroid tissue using SSB antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSB protein (Right) or empty vector (Left) detected with SSB antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSB protein using SSB antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKAR1B protein (Right) or empty vector (Left) detected with PRKAR1B antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKAR1B protein using PRKAR1B antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant VASP protein using VASP antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of VASP protein in paraffin embedded Human liver tissue using VASP antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VASP protein (Right) or empty vector (Left) detected with VASP antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant VASP protein (red) or empty vector (blue) stained using VASP antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant VASP protein using VASP antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VASP protein (Right) or empty vector (Left) detected with VASP antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant VASP protein (red) or empty vector (blue) stained using VASP antibody)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA40995, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA40995, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7369, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7369, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
IHC (Immunohiostchemistry) (Immunocytochemistry of ERGIC53 in HL60 cells with ERGIC53 antibody at 5 ?g/ml.)
WB (Western Blot) (Western blot analysis of ERGIC53 in HL60 cell lysate with ERGIC53 antibody at 1 ?g/mL in (A) the absence and (B) the presence of blocking buffer.)
WB (Western Blot) (Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Spinach, E.coli (GroEl), H. pylori, S. typhimurium, T. spiralis, Yeast, White Fly
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Pricing
Application Data (Figure 1. reactivity at the adhesion sites of CHO-cells transfected with E-Selectin. Dilution 500x.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of SERPINB2 protein in paraffin embedded Human Kidney tissue using SERPINB2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SERPINB2 protein (Right) or empty vector (Left) detected with SERPINB2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant OSM protein (Right) or empty vector (Left) detected with OSM antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of OSM protein in paraffin embedded Human lung tissue using OSM antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of POR protein in paraffin embedded Human pancreas tissue using POR antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant POR protein (Right) or empty vector (Left) detected with POR antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PECR protein using PECR antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of PECR protein in paraffin embedded Human liver tissue using PECR antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PECR protein (Right) or empty vector (Left) detected with PECR antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SLC2A5 protein (Right) or empty vector (Left) detected with SLC2A5 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SLC2A5 protein using SLC2A5 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDE2A protein (Right) or empty vector (Left) detected with PDE2A antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PDE2A protein (red) or empty vector (blue) stained using PDE2A antibody)
FCM/FACS (Flow Cytometry) (Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow cytometry analysis with 1 ug/mL Anti-TweakR (enavatuzumab) mAb (AAA47399) on Expi293 cells transfected with Human TweakR protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
ELISA (Figure 2. Flow cytometry analysis with 1 ug/mL Anti-TweakR (enavatuzumab) mAb (AAA47399) on Expi293 cells transfected with Human TweakR protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
Application Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag)
Application Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP)
Application Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 . Visualised using a LI-COR Odyssey infrared imaging system)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP)
Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜. Data represents mean+/-SEM of triplicates from a representative of 3 independent experiments.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Application Data (Published customer image: Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization. A) Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FceR? chain (right panel). B) Serum-opsonization inhibits the recognition of zymosan by both dectin-1 and dectin-2. NIH3T3 retrovirally transduced as indicated in the legend were incubated with zymosan that had been pre-incubated with serum at increasing concentrations and the interaction was monitored by flow cytometry. Data represents the mean+/-SEM of triplicates from a representative of 2 independent experiments. Data in (D) was analyzed by two-way ANOVA, with the significant Interaction statistics demonstrating the reduced binding of zymosan to the transduced cells after prior incubation with increasing concentrations of serum.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Application Data (Mouse spleen stained wiith Rat anti Mouse Dectin-2)
Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/M˜ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜ from dectin-1-deficient mice. All bars show mean+/-95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (˜C') or the use of different cell lineages (˜L'). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of PFKP protein in paraffin embedded Human Kidney tissue using PFKP antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PFKP protein (Right) or empty vector (Left) detected with PFKP antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using PFKP antibody (Red), compared to a nonspecific negative control antibody (Blue).)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human prostata tissues (A, B) using anti-NKX3A antibody with DAB staining.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human Liver tissues using NKX3A antibody)
WB (Western Blot) (Western Blot showing NKX3A antibody used against LNCaP (1) cell lysate.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant DTYMK protein using DTYMK antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of DTYMK protein in paraffin embedded Human prostate tissue using DTYMK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DTYMK protein (Right) or empty vector (Left) detected with DTYMK antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant CDK2 protein using CDK2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CDK2 protein (Right) or empty vector (Left) detected with CDK2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant EIF4E2 protein using EIF4E2 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of EIF4E2 protein in paraffin embedded Human prostate tissue using EIF4E2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant EIF4E2 protein (Right) or empty vector (Left) detected with EIF4E2 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CHEK2 protein in paraffin embedded Human liver tissue using CHEK2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
WB (Western Blot) (Figure-2: Western Blot analysis of Monkeypox L1R Antibody: Anti-Monkeypox L1R Antibody (Clone: ABM5H1.1D4) was used at 0.5 ug/ml on Monkeypox L1R Recombinant protein.)
ELISA (Figure-1: An indirect ELISA is carried out by coating L1R antigen in serial dilution from 5ng/well to 0.625ng/well and purified Anti-L1R monoclonal antibodies ABM5H1.1D4 was added at 100ng/well. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution)
Monoclonal Antibody to Human Osteopontin, C-terminal
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Specific for the C-terminal fragment of full-length Osteopontin. Recognizes native human Osteopontin from purified breast milk and recombinant murine OPN expressed in E.coli.
Applications
Western Blot
Purity
Protein A chromatography from ascites Purity: >95% pure determined by size-exclusion chromatography
FCM/FACS (Flow Cytometry) (Flow cytometry using the anti-CD40 antibody chi220 (AAA72191). Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of chi220 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody chi220 (AAA72191) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of chi220 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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