Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Immunohistochemistry of Hsp90 beta in paraffin-embedded Human breast cancer tissue using Hsp90 beta Rabbit mAb at dilution 1:50)
WB (Western Blot) (Western blot detection of Hsp90 beta in Hela,A549,HL-60 using Hsp90 beta Rabbit mAb(1:1000 diluted))
IHC (Immunohiostchemistry) (Immunohistochemistry of ALDH1A1 in paraffin-embedded Human Cholangiocarcinoma using ALDH1A1 Rabbit mAb at dilution 1:20)
WB (Western Blot) (Western blot detection of ALDH1A1 in K562,3T3 cell lysates using ALDH1A1 Rabbit mAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:55kDa.)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using beta Tubulin Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western blot with Anti beta Tubulin Monoclonal Antibody at dilution of 1:3000. Lane 1: HT-29 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: 293T cell lysate, Lane 4: Raw264.7 cell lysate, Lane 5: C6 cell lysate, Lane 6: Rat brain tissue lysate, Lane 7: Zebrafish tissue lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue using HP-1gamma Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma tissue using HP-1gamma Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) Hela, 2)3T3, 3) PC-12 cells using HP-1gamma Monoclonal Antibody at dilution of 1:1000.)
Application Data (Figure A. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD5 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD4 detected with Goat anti Mouse IgG1:RPE . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
WB (Western Blot) (Western blot analysis of untreated HeLa cell nuclear extract (20 ug; lane 1) or Hela nuclear extract treated with colcemid (20 ug; lane 2) probed with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) at a 1 ug/ul dilution.)
IF (Immunofluorescence) (Immunofluorescence staining of formaldehyde fixed mitotic DM4 cells with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) (left), counterstained with Hoechst (right).)
Application Data (Published customer image:Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 used to label cytokeratins in bovine recto-anal junction tissue sections by immunofluorescence.Image caption:Immunofluorescent images of tissue sections from RAJ-IVOC adherence assay trial 1. The RAJ-IVOC were inoculated with either O157 (106 CFU inoculum as shown in parenthesis) or not inoculated (no bacteria) and incubated at 39 °C for 3 or 24 h. Tissue sections of the RAJ-IVOC were then stained with immunofluorescent antibodies targeting the RAJ cells’ cytokeratins and O157, and images were recorded at 100× magnification. The adherent bacteria (shown with arrows), RAJ cells’ cytokeratins, and the nuclei have green, orange–red, and blue fluorescence, respectively. The squamous (S), junction (J, boxed), and columnar (C) regions of the RAJ are indicated along with a 100 um scale bar.)
Application Data (Published customer image:Mouse anti pan Cytokeratin antibody, clone C-11 (AAA50486) used to label cytokeratins in bovine cells by immunofluorescence.Image caption:Adherence patterns of EDL933 strains and mutants, obtained from sources A, B, and C, on RSE cells. The immunofluorescent stained slides are shown at 40x magnification. O157 have green fluorescence; RSE cells’ cytokeratins have orange-red fluorescence, and nuclei have blue fluorescence.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)
Monoclonal Antibody to Human Osteopontin, C-terminal
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Specific for the C-terminal fragment of full-length Osteopontin. Recognizes native human Osteopontin from purified breast milk and recombinant murine OPN expressed in E.coli.
Applications
Western Blot
Purity
Protein A chromatography from ascites Purity: >95% pure determined by size-exclusion chromatography
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody(AAA124523)AKT1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (AAA124523)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody (AAA124523).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1)
FCM/FACS (Flow Cytometry) (Staining of the A549 cell line with 0.5 ug of Purified Rat IgG2b Isotype Control (open histogram) or 0.5 ug of Purified anti-human CD104 (439-9B) (colored histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.)
IHC (Immunohiostchemistry) (Anti-Integrin Beta 4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
IHC (Immunohistochemistry) (Anti-Integrin Beta 4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (31G7) - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (31G7). Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (31G7).)
FCM/FACS (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)
IF (Immunofluorescence) (Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SF3B1 with AAA62337 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (SF3B1 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with AAA62337 of 10 ug. Western blot was performed from the immunoprecipitate using AAA62337 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : SF3B1 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 146 KDa Observed band size : 146 KDa Blocking/Dilution buffer : 1x TBST.)
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Factor XIIIa Mouse Monoclonal Antibody (F13A1/1683)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified Factor XIIIa Mouse Monoclonal Antibody (F13A1/1683).Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Histiocytoma stained with Factor XIIIa Monoclonal Antibody (F13A1/1683).)
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control))
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of Ramos cellsusing AF488-labeled Lambda Light Chain Monoclonal Antibody (LcN-2) (Green). DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot) (Western Blot of human Intestinal Lysateusing Lambda Light Chain Monoclonal Antibody (LcN-2).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Lambda Light Chain Monoclonal Antibody (LcN-2).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RAB1B with M25008 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (AB1B was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25008 of 10 ug.ution buffer and concentration:5% milk/TBST. Western blot was performed from the immunopre-cipitate using M25008 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (All lanes : RAB1B Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 22 KDa Observed band size : 22 KDa Blocking/Dilution buffer : 1x TBST.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HeLa cells using anti-XRCC1 antibody (AAA126873).Overlay histogram showing HeLa cells stained with AAA126873 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-XRCC1 Antibody (AAA126873, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 2. IF analysis of XRCC1 using anti-XRCC1 antibody (AAA126873).XRCC1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-XRCC1 Antibody (AAA126873) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of XRCC1 using anti-XRCC1 antibody (AAA126873).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human HEL whole cell lysates,Lane 6: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XRCC1 antigen affinity purified monoclonal antibody (#AAA126873) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for XRCC1 at approximately 95 kDa. The expected band size for XRCC1 is at 69 kDa.)
IHC (Immunohistochemisry) (Figure 3. IHC analysis of KLHL12 using anti-KLHL12 antibody (AAA126956).KLHL12 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-KLHL12 Antibody (AAA126956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of KLHL12 using anti-KLHL12 antibody (AAA126956).KLHL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-KLHL12 Antibody (AAA126956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of KLHL12 using anti-KLHL12 antibody (AAA126956).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KLHL12 antigen affinity purified monoclonal antibody (#AAA126956) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 63 kDa. The expected band size for KLHL12 is at 63 kDa.)
IHC (Immunohistochemisry) (Figure 2. IHC analysis of ERBB2 using anti-ERBB2 antibody (M00010-1).ERBB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERBB2 Antibody (M00010-1) overnight at 4 degree C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of ERBB2 using anti-ERBB2 antibody (M00010-1).ERBB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERBB2 Antibody (M00010-1) overnight at 4 degree C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of ERBB2 using anti-ERBB2 antibody (M00010-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERBB2.)
>95%; Protein A or G purified from cell culture supernatant.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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