Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TUBA3E protein using TUBA3E antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBA3E protein (Right) or empty vector (Left) detected with TUBA3E antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SRR protein using SRR antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of SRR protein in paraffin embedded Human colon tissue using SRR antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SRR protein (Right) or empty vector (Left) detected with SRR antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SNX9 protein using SNX9 antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of SNX9 protein in paraffin embedded Human prostate tissue using SNX9 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SNX9 protein (Right) or empty vector (Left) detected with SNX9 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SNX9 protein (red) or empty vector (blue) stained using SNX9 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of PROM2 protein in paraffin embedded Human liver tissue using PROM2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PROM2 protein (Right) or empty vector (Left) detected with PROM2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SulT1A1 protein using SulT1A1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SulT1A1 protein (Right) or empty vector (Left) detected with SulT1A1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SulT1A1 protein (red) or empty vector (blue) stained using SulT1A1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PTPRE protein using PTPRE antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PTPRE protein (Right) or empty vector (Left) detected with PTPRE antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PTPRE protein (red) or empty vector (blue) stained using PTPRE antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUDT6 protein using NUDT6 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUDT6 protein (Right) or empty vector (Left) detected with NUDT6 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NUDT6 protein (red) or empty vector (blue) stained using NUDT6 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant FGF21 protein using FGF21 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FGF21 protein (Right) or empty vector (Left) detected with FGF21 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant HSPBP1 protein using HSPBP1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSPBP1 protein (Right) or empty vector (Left) detected with HSPBP1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant FRK protein using FRK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FRK protein (Right) or empty vector (Left) detected with FRK antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RNF144B protein using RNF144B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RNF144B protein (Right) or empty vector (Left) detected with RNF144B antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)
IF (Immunofluorescence) (BF0229 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-mouse IgG (H+L) Ab, diluted at 1/600, was used as secondary Ab.)
WB (Western Blot) (Figure 1: Western blot analysis using LAMB1 mouse mAb against truncated LAMB1-His recombinant protein (1).)
WB (Western Blot) (Figure 1: Western blot analysis using anti-CDC25C mAb against Hela (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.)
WB (Western Blot) (Western Blot Analysis of HCT116 Cell lysate using Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).)
SDS-PAGE (SDS-PAGE Analysis of Purified Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).)
S100B (Astrocyte and Melanoma Marker) Mouse Monoclonal Antibody [Clone S100B/4138]
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human
Applications
Immunohistochemistry
Pricing
WB (Western Blot) (Figure 1: Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.)
WB (Western Blot) (HeLa cells were subjected to SDS PAGE followed by western blot with AAA247937 (HSP60 Antibody) at dilution of 1:20000)
IP (Immunoprecipitation) (IP Result of anti-HSP60 (IP: AAA247937, 5ug; Detection: AAA247937 1:2000) with mouse liver tissue lysate 4000ug.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using AAA247937 (HSPD1 Antibody) at dilution of 1:50)
IF (Immunofluorescence) (Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
IHC (Immunohistochemisry) (IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysateAll lanes: SF3B1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 146, 17 kDaObserved band size: 130 kDa)
WB (Western Blot) (Detection of human MEK1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Hep-G2, Jurkat, A-549, HEK293T, RKO, U2OS, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 .)
WB (Western Blot) (Detection of mouse MEK1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, C2C12, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 .)
IP (Immunoprecipitation) (Detection of human MEK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used for IP at 20 ul/mg lysate. MEK1 was also immunoprecipitated by rabbit anti-MEK1 recombinant monoclonal antibody . For blotting immunoprecipitated MEK1, AAA213573 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry) (Detection of human MEK1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK1 recombinant monoclonal (AAA213573 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Detection of human AKT1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, A-549, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (AAA213660 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot) (Detection of mouse AKT1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (AAA213660 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
FCM/FACS (Flow Cytometry) (Detection of human AKT1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (AAA213660) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Detection of mouse AKT1 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (AAA213660) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Monoclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
Gene Names
STAT3; APRF; HIES
Reactivity
Human, Mouse, Rat, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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