Mouse HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) Monoclonal Antibody | anti-HIF1A antibody

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)

Cat. Num. AAA215356

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Reactivity: Human, Mouse, Chicken
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: HIF1 alpha (Hypoxia-Inducible Factor 1-alpha), Antibody; HIF1 alpha (Hypoxia-Inducible Factor 1-alpha); ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS superfamily 1 (MOP1); PAS domain-containing protein 8 (PASD8); anti-HIF1A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Chicken
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: Ha111a
• Specificity: HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 826

• Applicable Applications for anti-HIF1A antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Immunogen: Synthetic peptide corresponding to Human HIF-1 alpha aa 239-530
• Cellular Localization: Nuclear and Cytoplasmic
• Hu-chromosome Location: 14q23.2
• Positive Control: Cobalt chloride treated HeLa cells. Breast or Bladder carcinomas.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human placenta stained with HIF1 alpha Mouse Monoclonal Antibody (Ha111a).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human placenta stained with HIF1 alpha Mouse Monoclonal Antibody (Ha111a).)

WB (Western Blot)

(Western Blot Analysis of human spleen tissue lysate using HIF1 alpha Mouse Monoclonal Antibody (Ha111a).)

References

Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. | Li P, et al. MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1a. Exp Ther Med 10:1165-1170 (2015).

NCBI and Uniprot Product Information

• NCBI GI #: 4504385
• NCBI GeneID: 3091
• NCBI Accession #: NP_001521.1
• NCBI GenBank Nucleotide #: NP_001521.1
• UniProt Accession #: Q16665
• Molecular Weight: 92-110kDa
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 subunit alpha
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
• UniProt Entry Name: HIF1A_HUMAN