Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ID1 mAb. [Lot No. 2456C1a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ID1 monoclonal antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody(AAA46888)CCND1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCND1 Antibody (AAA46888)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of Cyclin D1 expression in (1)MCF-7 cell lysates;(2) LnCaP cell lysates (AAA46888).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCND1)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human uterus, using Integrin alpha 6 Antibody(AAA47073)ITGA6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGA6 Antibody (AAA47073)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of Integrin alpha 6 expression in SW480 cell lysate (AAA47073).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGA6)
WB (Western Blot) (Western blot analysis of Lamin B1 expression in (1) Hela cell lysate; (2) Jurkat cell lysate; (3) Mouse brain lysate; (4) Rat heart lysate with Lamin B1 Antibody (AAA47119).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMNB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LMNB1)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat liver, using Lamin B1 Antibody(AAA47119)LMNB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMNB1 Antibody (AAA47119)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
FCM/FACS (Flow Cytometry) (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
Application Data (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
Application Data (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
WB (Western Blot) (Western blot of extracts from HeLa cells, using anti-SIK2, clone S15G10.)
ICC (Immunocytochemistry) (Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.)
IHC (Immunohistochemistry) (Anti-SIK2 / SNF1LK2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
DB (Dot Blot) (Histone H3K9me0 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 ug/mL. Lane 1: Histone H3 unmodified. Lane 2: Histone H3K4me3. Lane 3: Histone H3K9me3. Lane 4: Histone H3 K9ac. Lane 5: Histone H3 K14ac. Lane 6: Histone H3K4ac. Lane 7: Histone H3 S10ph.)
WB (Western Blot) (Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.)
Application Data (Histone H4K8ac antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K8ac antibody (mAb) was applied at a dilution of 0.7 ug/ml to MODified Histone Peptide Array tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K8ac antibody (mAb) for acetyl-Lys8 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K8ac antibody (mAb) at a dilution of 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide. Lane 9: acetyl-Lys20 peptide. Lane 10: unmodified Lys20 peptide. Lane 11: acetyl-Lys31 peptide. Lane 12: unmodified Lys31 peptide.)
WB (Western Blot) (Histone H4K8ac antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4K8ac (mAb) at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: cells treated with sodium butyrate.)
ChIP (Chromatin Immunoprecipitation) (Histone H4K8ac antibody (mAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4K8ac (mAb) or the equivalent amount of mouse IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Application Data (Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD3)
Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Application Data (Published customer image: FITC conjugated Mouse anti Rat CD71 antibody, clone OX-26 used for flow cytometryImage caption:Flow cytometric analysis of follicle stem cells separated by Vario MACS. (a -d) Single-color flow cytometric analysis of the selected follicle stem cells. X axis represents fluorescence intensity, and Y axis represents cell number. (a) Bulge region cell suspension without antibody staining was used as control. (b) CD34bri cells were isolated by Vario MACS. (c) Control for a6 separation. (d) a6bri cells were isolated by Vario MACS. (e -h) Two-color flow cytometric analysis of follicle stem cells selected by Vario MACS. Cells were divided into four subpopulations: a PEbri FITCdim cells; b PEbri FITCbri cells; c PEdim FITCdim cells; and d PEdim FITCbri cells. (e) Control. (f) CD71 and CD34 was detected with FITC (X axis) and with PE (Y axis). The majority of the isolated cells were CD34briCD71dim cells, characterized by high level expression of CD34 and low level of CD71. No CD34dimCD71bri cells were detected in our experiments. (g) Control for a6 and CD71 combined separation; (h) The a6briCD71dim cells were the main subpopulation of the separated cells.From: Huang E, Lian X, Chen W, Yang T, Yang L. Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system. Acta Histochem Cytochem. 2009 Oct 30;42(5):129-36.)
Application Data (Staining of rat spleen lymphocytes with Mouse anti Rat CD71)
Application Data (Staining of rat spleen cells with Mouse anti Rat CD71:RPE)
Application Data (Staining of mouse spleen with Mouse anti Rat CD71:Biotin)
Application Data (Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant GPHN protein using GPHN antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GPHN protein (Right) or empty vector (Left) detected with GPHN antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant HDAC10 protein using HDAC10 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HDAC10 protein (Right) or empty vector (Left) detected with HDAC10 antibody)
FCM/FACS (Flow Cytometry) (Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Purified from cell culture supernatant by affinity chromatography
Pricing
Application Data (Gentamicin is an antibiotic used to treat several types of bacterial infections including bone infections, endocarditis, pelvic inflammatory disease, meningitis, pneumonia, urinary tract infections, and sepsis. Gentamicin was discovered in 1963 and is produced by Micromonospora purpurea. Gentamicin is on the World Health Organization's List of Essential Medicines.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANKRD53 protein using ANKRD53 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ANKRD53 protein in paraffin embedded Carcinoma of Human thyroid tissue using ANKRD53 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANKRD53 protein (Right) or empty vector (Left) detected with ANKRD53 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant UBE2T protein (Right) or empty vector (Left) detected with UBE2T antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant UBE2T protein using UBE2T antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RDH11 protein using RDH11 antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of RDH11 protein in paraffin embedded Carcinoma of Human liver tissue using RDH11 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RDH11 protein (Right) or empty vector (Left) detected with RDH11 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RDH11 protein (red) or empty vector (blue) stained using RDH11 antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of RBBP7 protein in paraffin embedded Human endometrium tissue using RBBP7 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RBBP7 protein (Right) or empty vector (Left) detected with RBBP7 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RBBP7 protein (red) or empty vector (blue) stained using RBBP7 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SPG7 protein using SPG7 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SPG7 protein (Right) or empty vector (Left) detected with SPG7 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SPG7 protein (red) or empty vector (blue) stained using SPG7 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant FAM40A protein using FAM40A antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of FAM40A protein in paraffin embedded Human thyroid tissue using FAM40A antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FAM40A protein (Right) or empty vector (Left) detected with FAM40A antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of DPP9 protein in paraffin embedded Human lymphoma tissue using DPP9 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DPP9 protein (Right) or empty vector (Left) detected with DPP9 antibody)
IF (Immunofluorescence) (ICC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1,. Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20 degree C . Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: DAKO LSAB2 streptavidin-peroxidase system. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Left: control; Right: 24 hours after 7th passage of senescence. Courtesy of: Valentina di Felice, University of Palermo, Italy.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
WB (Western Blot) (Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20 degree C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NPR3 protein using NPR3 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of NPR3 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using NPR3 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NPR3 protein (Right) or empty vector (Left) detected with NPR3 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PAICS protein using PAICS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PAICS protein (Right) or empty vector (Left) detected with PAICS antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PAICS protein (red) or empty vector (blue) stained using PAICS antibody)
WB (Western Blot) (Western blot analysis of different tissue and cell lysates using mouse mAb to GAP43, MO22170, dilution 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP43 protein.)
IF (Immunofluorescence) (Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, AAA76610, dilution 1:1,000, in red, and costained with chicken pAb to MAP2, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons)
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Purity
Affinity Purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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