Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohistochemisry) (Immunohistochemistry of FMRP in paraffin-embedded Human lung cancer tissue using FMRP Rabbit mAb at dilution 1/50)
IF (Immunofluorescence) (Immunofluorescence of FMRP (green) in Hela using FMRP Rabbit mAb at dilution 1/5, and DAPI(blue))
WB (Western Blot) (Western blot detection of FMRP in 293,U2OS,Jurkat,COS7 cell lysates using FMRP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:70-80kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of CDA in paraffin-embedded Human colon cancer tissue using CDA Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of CDA in Hela cell lysates using CDA Rabbit mAb(1:1000 diluted).Predicted band size:16kDa.Observed band size:16kDa.)
WB (Western Blot) (Detection of mouse BRD7 by western blot. Samples: Whole cell lysate (10 ug) from CT26, NIH 3T3, CH27, A20, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD7 recombinant monoclonal antibody (AAA213646 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human BRD7 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD7 recombinant monoclonal antibody (AAA213646 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
FCM/FACS (Flow Cytometry) (Detection of human BRD7 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-BRD7 recombinant monoclonal antibody (AAA213646) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Detection of mouse BRD7 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-BRD7 recombinant monoclonal antibody (AAA213646) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-lung-cancer tissue. 1.Transferrin Monoclonal Antibody(7F4)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Transferrin Monoclonal Antibody(7F4) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of Human serum. mAb diluted at 1:2000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human LiverTissue using AMPK ?2 Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)293T Cell. 2) Mouse Brain Tissue Lysate using AMPK?2Mouse Monoclonal mAb diluted at 1:2.000.)
WB (Western Blot) (Western blot detection of CDK4 in Hela.Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.)
WB (Western Blot) (Western blot detection of CDK4 in Hela.C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.)
WB (Western Blot) (Western blot detection of AMPK beta 1 in 3T3.Hela.PC-12.COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-Ku70 antibody (dilution 1:200).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Ku70 mouse mAb.)
WB (Western Blot) (Western blot detection of Ku70 in Hela.A549.COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).)
WB (Western Blot) (Western blot detection of FEN-1 in Hela.Jurkat.3T3.COS7.PC-12.C6.Raw264.7.Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
WB (Western Blot) (Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody)
Application Data (Diluted at 1:10000)
WB (Western Blot) (Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody)
IF (Immunofluorescence) (Immunofluorescence analysis of 293T transfected DYKDDDDK and GFP tag protein. 1, DYKDDDDK primary Antibody(red AAA320531) was diluted at 1:1000(4 degree C overnight), GFP primary Antibody(green C40014) was diluted at 1:1000(4 degree C overnight),)
WB (Western Blot) (Western blot analysis of PC3 Cell Lysate using Phospho-Akt Ser473 Mouse mAb diluted at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PTEN (PT2124) antibody. The HRP-conjugated Goat anti-M)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with FGFR4-hIgGFc using FGFR-4 Monoclonal Antibody (green). showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot) (Western Blot analysis using FGFR-4 Monoclonal Antibody against extracellular domain of human FGFR-4 (aa22-369).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Fibrinogen ? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using Fibrinogen ? Monoclonal Antibody against human Fibrinogen ? (AA: 210-437) recombinant protein.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human liver cancer tissue using ABCB5 Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using ABCB5 Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) Hela, 2) 293T cells using ABCB5 Monoclonal Antibody at dilution of 1:2000.)
FCM/FACS (Flow Cytometry) (C57BL/6 murine bone marrow cells are stained with Anti-Mouse Gr-1 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained bone marrow cells (empty black histogram) are used as control.)
IHC (Immunohiostchemistry) (Immunohistochemistry of GNB2 in paraffin-embedded Human Cholangiocarcinoma using GNB2 Rabbit mAb at dilution 1:20)
WB (Western Blot) (Western blot detection of GNB2 in Rat Brain,C6,3T3,Hela cell lysates using GNB2 Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:32kDa.)
IF (Immunofluorescence) (Figure 2. IF analysis of HSD3B1/B2 using anti-HSD3B1/B2 antibody (AAA126920).HSD3B1/B2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-HSD3B1/B2 Antibody (AAA126920) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of HSD3B1/B2 using anti-HSD3B1/B2 antibody (AAA126920).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSD3B1/B2 antigen affinity purified monoclonal antibody (#AAA126920) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSD3B1/B2 at approximately 42 kDa. The expected band size for HSD3B1/B2 is at 42 kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of HMGB2 in paraffin-embedded Human tonsil using HMGB2 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of HMGB2 in CHO-K1, Ramos cell lysates using HMGB2 Rabbit mAb(1:1000 diluted).Predicted band size:24kDa.Observed band size:24kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of EpCAM in paraffin-embedded Human breast cancer tissue using EpCAM Rabbit mAb at dilution 1/50)
IF (Immunofluorescence) (Immunofluorescence of EpCAM (green) in MCF-7 using EpCAM antibody at dilution 1/2, and DAPI(blue))
WB (Western Blot) (Western blot detection of EpCAM in MCF7,HCT116,T47D,Hela cell lysates using EpCAM Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:40kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Niemann Pick C2 in paraffin-embedded Human lung cancer tissue using Niemann Pick C2 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Niemann Pick C2 in C6,3T3,Hela cell lysates using Niemann Pick C2 Rabbit mAb(1:1000 diluted).Predicted band size:17kDa.Observed band size:17kDa.)
Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.