Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (RNA pol II phospho Ser5 antibody (mAb) (Clone 1H4B6) tested by Western blot. HeLa nuclear extract (40 ug per lane) probed with RNA pol II phospho Ser5 antibody (mAb) at a 1 ug/ml dilution.)
IF (Immunofluorescence) (RNA pol II phospho Ser5 antibody (mAb) (Clone 1H4B6) tested by Immunofluorescence. Left: HeLa cell stained with RNA pol II CTD phospho Ser5 antibody (mAb). Middle: DAPI. Right: Merge.)
ICC (Immunocytochemistry) (Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot) (Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.)
IF (Immunofluorescence) (ImmunofluorescencestainingofLNCaPcellswithanti-PSMAantibodyJ591(AAA72548). ImmunofluorescenceanalysisofparaformaldehydefixedLNCaPcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofJ591(AAA72548)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72548,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of live Jurkat cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)
SDS_PAGE (SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/964). Confirmation of Integrity and Purity of Antibody.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of live Jurkat cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)
IF (Immunofluorescence) (Immunofluorescence Analysis of live Jurkat cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-mouse IgG-CF488 (green). The nuclear counterstain is RedDot.)
IF (Immunofluorescence) (Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (PHE5))
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Small Cell Lung Carcinoma stained with Chromogranin A Monoclonal Antibody (LK2H10 + PHE5))
FCM/FACS (Flow Cytometry) (Flow-cytometry using the Anti-IL-9R antibody AH9R2 . Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- phospho-Synapsin �� (S9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- phospho-Synapsin �� (S9) antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemistry of ATG4C in paraffin-embedded Human lung cancer tissue using ATG4C Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of ATG4C in K562,Hela cell lysates using ATG4C Rabbit mAb(1:1000 diluted).Predicted band size:53kDa.Observed band size:53kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of DCAMKL1 in paraffin-embedded Human breast cancer tissue using DCAMKL1 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of DCAMKL1 in Rat Brain lysates using DCAMKL1 Rabbit mAb(1:1000 diluted).Predicted band size:82kDa.Observed band size:82kDa.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Pancreatic Carcinoma using Cyclophilin BMouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma using Cyclophilin BMouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1) Hela, 2)Jurkat, 3)293T, 4)Rat Liver Tissue, 5) 3T3, 6) HepG2 with Cyclophilin B Mouse mAb diluted at 1:2,000.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Daudi cells with CD27 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CD27 in K562 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD27 on Raji cell using anti-CD27 antibody at 1/500 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of PC3 Cell Lysate using Phospho-Akt Ser473 Mouse mAb diluted at 1:1000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of CDC42EP1 on different lysates using anti-CDC42EP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: HUVEC Lane 2: Hela)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution. Positive control: Lane 1: MCF-7 Lane 2: A549 Lane 3: Hela Lane 4: HepG2 Lane 5: SKBR3 Lane6: SKOV3 Lane 7: SW1990)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
WB (Western Blot) (Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: T47D Lane3: MOLT4 Lane4: Raji)
WB (Western Blot) (Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD16/32 Monoclonal Antibody(PE/Cy5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
FCM/FACS (Flow Cytometry) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD127 Monoclonal Antibody(PE/Cy5 Conjugated)(left). Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(Alexa Fluor 488 Conjuaged)(right) are used as control.)
IHC (Immunohiostchemistry) (Immunohistochemistry of PRMT1 in paraffin-embedded Human breast cancer tissue using PRMT1 Rabbit mAb at dilution 1/20)
WB (Western Blot) (Western blot detection of PRMT1 in K562,C6 cell lysates using PRMT1 Rabbit mAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of Smac/Diablo in paraffin-embedded Human tonsil using Smac/Diablo Rabbit mAb at dilution 1/5)
ICC (Immunocytochemistry) (Immunocytochemistry of Smac/Diablo(green) in Hela cells using Smac/Diablo Rabbit mAb at dilution 1/200, and DAPI(blue))
WB (Western Blot) (Western blot detection of Smac/Diablo in K562,Hela cell lysates using Smac/Diablo Rabbit mAb(1:1000 diluted).Predicted band size:27kDa.Observed band size:21kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Eph receptor B4/HTK in paraffin-embedded Human breast cancer tissue using Eph receptor B4/HTK Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Eph receptor B4/HTK in Hela,A549,C6 cell lysates using Eph receptor B4/HTK Rabbit mAb(1:1000 diluted).Predicted band size:108kDa.Observed band size:135kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Histone H3.3 in paraffin-embedded Human lung cancer tissue using Histone H3.3 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Histone H3.3 in K562,C6,3T3 cell lysates using Histone H3.3 Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.)
IF (Immunofluorescence) (Immunofluorescence of MRPS26(green) in Hela cells using MRPS26 Rabbit mAb at dilution 1/50, and DAPI(blue))
WB (Western Blot) (Western blot detection of MRPS26 in K562,Hela cell lysates using MRPS26 Rabbit mAb(1:1000 diluted).Predicted band size:24kDa.Observed band size:24kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of Paxillin in paraffin-embedded Human breast cancer tissue using Paxillin Rabbit mAb at dilution 1/50)
IF (Immunofluorescence) (Immunofluorescence of Paxillin (green) in hela using Paxillin Rabbit mAb at dilution 1/5, and DAPI(blue))
WB (Western Blot) (Western blot detection of Paxillin in C6 cell lysates using Paxillin Rabbit mAb(1:1000 diluted).Predicted band size:65kDa.Observed band size:65kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of PKA R2 (Phospho-Ser99) in paraffin-embedded Human tonsil using PKA R2 (Phospho-Ser99) Rabbit mAb at dilution 1/100)
WB (Western Blot) (Western blot detection of Phospho-PKA RIIalpha (Ser99) in Rat Brain,C6 cell lysates using Phospho-PKA RIIalpha (Ser99) Rabbit mAb(1:1000 diluted).Predicted band size:46kDa.Observed band size:50kDa.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using FMR1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissues. showing cytoplasmic localization with DAB staining using FMR1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using FMR1 Monoclonal Antibody against Jurkat (1) and K562 (2) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded cerebellum tissues (left) and lung cancer (right) with DAB staining using ALDH1A1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ALDH1A1 Monoclonal Antibody against Raji (1). Jurkat (2). THP-1 (3) and K562 (4) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human Liver tissues with AEC staining using ApoA Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ApoA Monoclonal Antibody against truncated LPA-His recombinant protein (1) and truncated Trx-LPA(aa4330-4521) recombinant protein (2).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells using Oct-3/4 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of NTERA-2 cells using Oct-3/4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using Oct-3/4 Monoclonal Antibody against NTERA-2 (1) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using OTX2 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using OTX2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) with DAB staining using OTX2 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using OTX2 Monoclonal Antibody against HepG2 (1). Jurkat (2). and NTERA-2 (3) cell lysate.)
Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.