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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using ARRB1 Antibody(AAA47099)ARRB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARRB1 Antibody (AAA47099)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of ARRB1 expression in 293T cell lysate (AAA47099).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARRB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARRB1)
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Affinity-chromatography
Pricing
WB (Western Blot) (Western blot analysis of TIA1 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate (AAA47118).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIA1 monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TIA1)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human IL-17RA protein, His tagged protein PME100468 can bind Rabbit anti-IL-17RA monoclonal antibody (clone: DM126) in a linear range of 0. 3-15 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human IL-17RA protein, His tagged protein PME100468 can bind Rabbit anti-IL-17RA monoclonal antibody (clone: DM126) in a linear range of 0. 3-15 ng/ml.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.)
WB (Western Blot) (Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
WB (Western Blot) (Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).)
WB (Western Blot) (Western Blot analysis of Human Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
WB (Western Blot) (Western blot analysis of GRP78 BiP expression in (1) LnCaP cell lysate;(2) HepG2 cell lysate (AAA47008).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA5)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody(AAA47008)HSPA5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA5 Antibody (AAA47008)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of p16 Arc expression in (1)HeLa cell lysate; (2)HepG2 cell lysate (AAA47013).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAA1)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using AMPK alpha 1 Antibody(AAA47013)PRKAA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKAA1 Antibody (AAA47013)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining .)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.)
WB (Western Blot) (U-937 cells were subjected to SDS PAGE followed by western blot with AAA102707 (BCL2 Antibody) at dilution of 1:6000)
Application Data
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA102707 (BCL2 Antibody) at dilution of 1:100)
WB (Western Blot) (The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: A431 cell lysate;;)
ICC (Immunocytochemistry) (ICC/IF analysis of EGFR in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUCB1 protein (Right) or empty vector (Left) detected with NUCB1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUCB1 protein using NUCB1 antibody)
WB (Western Blot) (Western Blot analysis using DYKDDDDK antibodyWestern Blot showing DYKDDDDK antibody used against two different fusion protein (1), (2) with DYKDDDDK tag.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant C16orf72 protein using C16orf72 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant C16orf72 protein (Right) or empty vector (Left) detected with C16orf72 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BDH2 protein using BDH2 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BDH2 protein in paraffin embedded Human pancreas tissue using BDH2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BDH2 protein (Right) or empty vector (Left) detected with BDH2 antibody)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Vinculin Monoclonal Antibody (VCL/2573). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified Vinculin Mouse Monoclonal Antibody (VCL/2573). Confirmation of Purity and Integrity.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Mouse Monoclonal Antibody (VCL/2573).)
ICC (Immunocytochemistry) (ICC staining Phospho-EGFR (Y1092) in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-EGFR (Y1092) antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-EGFR (Y1092) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-EGFR (Y1092) on different lysates using anti-Phospho-EGFR (Y1092) antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 treated with EGF Lane 2: Untreated A431)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Gamma Tubulin Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Gamma Tubulin Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western Blot analysis of paraffin-embedded JurkatHelaMouse-brainRat-brain using Gamma Tubulin Mouse mAb diluted at 1:1000.)
WB (Western Blot) (Detection of human Interferon alpha/beta receptor 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, RPMI-8226, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (AAA213673 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohiostchemistry) (Detection of human Interferon alpha/beta receptor 1 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (AAA213673 lot 1) used for IP at 15 ul/mg lysate. Interferon alpha/beta receptor 1 was also immunoprecipitated by a second antibody against a different epitope of Interferon alpha/beta receptor 1 . For blotting immunoprecipitated Interferon alpha/beta receptor 1, AAA213673 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
FCM/FACS (Flow Cytometry) (Detection of human Interferon alpha/beta receptor 1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (AAA213673) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Ndufs4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining Asparagine synthetase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Asparagine synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Asparagine synthetase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Asparagine synthetase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Asparagine synthetase on different lysates using anti-Asparagine synthetase antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Human skeletal muscle)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD38 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD38 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD38 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD38 on different lysates using anti-CD38 antibody at 1/1, 000 dilution. Positive control: Lane 1: THP-1 Lane 2: Raji)
ICC (Immunocytochemistry) (ICC staining Cdk4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk4 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of Cdk4 on different lysates using anti-Cdk4 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: K562)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Protein Kinase D2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Protein Kinase D2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Protein Kinase D2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Protein Kinase D2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Protein Kinase D2 on A549 cell using anti-Protein Kinase D2 antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with GABA B Receptor 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GABA B Receptor 2 on mouse cerebellum tissue lysate using anti- GABA B Receptor 2 antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Biglycan antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Biglycan on mouse heart and human skin tissue lysates using anti-Biglycan antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with LPlunc1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining LPlunc1 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded tonsil tissue using anti-LPlunc1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded brain tissue using anti-LPlunc1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LPlunc1 on human LPlunc1 recombinant protein using anti-LPlunc1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with CD31 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD31 on THP-1 cell lysates using anti-CD31 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Integrin alpha V antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Integrin alpha V+beta 6 on different lysates using anti-Integrin alpha V+beta 6 antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: PC-12)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Integrin alpha 6 on Raji cell lysates using anti-Integrin alpha 6 antibody at 1/500 dilution.)
ICC (Immunocytochemistry) (ICC staining Hsc70 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Hsc70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsc70 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsc70 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Hsc70 on different lysates using anti-Hsc70 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: PC-12)
Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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