Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Immunohistochemistry of CD32B in paraffin-embedded Human tonsil using CD32B Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of CD32B in C6 cell lysates using CD32B Rabbit mAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:44kDa.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LY75 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-LY75 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-LY75 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Myosin heavy chain antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Myosin heavy chain on human skeletal muscle lysates using anti-Myosin heavy chain antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using gamma Tutulin Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using gamma Tutulin Mouse mAb diluted at 1:200.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody)
ICC (Immunocytochemistry) (ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Bad antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Bad in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Bad in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of Bad on MCF-7 cell lysates using anti-Bad antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with SHC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining SHC1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of SHC1 on NIH/3T3 cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of SHC1 on HEK293 (1) and SHC1-hIgGFc transfected HEK293 (2) cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of SHC1 on human SHC1 recombinant protein using anti-SHC1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with KIF22 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- KIF22 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- KIF22 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of KIF22 on HEK293 (1) and KIF22-hIgGFc transfected HEK293 (2) cell lysate using anti- KIF22 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of KIF22 on human KIF22 recombinant protein using anti- KIF22 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human skin tissue with Phospho-MLKL (Ser358) Monoclonal Antibody at dilution of 1:200)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma tissue with Phospho-MLKL (Ser358) Monoclonal Antibody at dilution of 1:200)
FCM/FACS (Flow Cytometry) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD11c Monoclonal Antibody(AF488 Conjugated) and Anti-Mouse MHC II (I-A/I-E) Monoclonal Antibody(APC Conjugated). Splenocytes stained with Anti-Mouse MHC II (I-A/I-E) Monoclonal Antibody(APC Conjugated)(Right) are used as control)
IF (Immunofluorescence) (Immunofluorescence of EpCAM (green) in MCF-7 using EpCAM antibody at dilution 1:20, and DAPI(blue))
IHC (Immunohiostchemistry) (Immunohistochemistry of EpCAM in paraffin-embedded Human breast cancer tissue using EpCAM Rabbit mAb at dilution 1:50)
WB (Western Blot) (Western blot detection of EpCAM in MCF7,HCT116,T47D,Hela cell lysates using EpCAM Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:40kDa.)
WB (Western Blot) (Western blot detection of Placental alkaline phosphatase (PLAP) in Hela cell lysates using Placental alkaline phosphatase (PLAP) Rabbit mAb(1:500 diluted).Predicted band size:58kDa.Observed band size:70kDa.)
WB (Western Blot) (Western blot detection of Placental alkaline phosphatase (phospho LAP) in Hela cell lysates using Placental alkaline phosphatase (phospho LAP) Rabbit mAb(1:1000 diluted).Predicted band size:58KDa.Observed band size:70KDa.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using MMP-1 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using MMP-1 Monoclonal Antibody. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) with DAB staining using MMP-1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using MMP-1 Monoclonal Antibody against HEK293 (1) and MMP1-hIgGFc transfected HEK293 (2) cell lysate.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using Human IgG-Fc Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot) (Western Blot analysis using Human IgG-Fc Monoclonal Antibody against different fusion proteins with human IgG (Fc specific) tag.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-SFRS3 (PTR1361) antibody. The HRP-conjugated Goat anti-Ra)
IF (Immunofluorescence) (Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red (Red).)
WB (Western Blot) (Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AKR1C1 Lane 2. : HepG2 cell lysate Lane 3. : Raji cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of SBDS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SBDS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SBDS antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of ING2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ING2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ING2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of Peroxiredoxin3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Peroxiredoxin3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The recombinant human PRDX3 protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: TF1 cell lysate Lane 5.: U87MG cell lysate Lane 6.: Raji cell lysate Lane 7.: 293T cell lysate Lane 8.: Jurkat cell lysate Lane 9.: MCF7 cell lysate)
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot) (The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant hGH (21-217aa) Lane 2.: 20kDa hGH (27-202aa))
IF (Immunofluorescence) (ICC/IF analysis of CD29 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD29 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (Cell lysates of U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD29 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry) (Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IGJ protein (Right) or empty vector (Left) detected with IGJ antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant IGJ protein (red) or empty vector (blue) stained using IGJ antibody)
IF (Immunofluorescence) (ICC/IF analysis of DECR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DECR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DECR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: HepG2 cell lysate)
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot) (The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A H3N2/HA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant Influenza A H1N1/HA protein;;Lane 2.: Recombinant Influenza A H5N2/HA protein;;Lane 3.: Recombinant Influenza A H3N2/HA protein;;)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7371, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7371, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of Copper Transporting ATPase 1 protein using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 . Lane 1: MW Ladder. Lane 2: 10 ug SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 . Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 1 Antibody. (D) Composite.)
Immunoprecipitation, Western Blot, Immunohistochemistry
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.