Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG.)
WB (Western Blot) (Western blot of extracts (35 ug) from 9 different cell lines by using anti-ENG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
IF (Immunofluorescence) (Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody.)
IHC (Immunohistochemistry) (Anti-CD105 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
WB (Western Blot) (Cell lysates of mouse brain (60 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohiostchemistry) (Anti-FABP7 / BLBP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry) (Anti-FABP7 / BLBP antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling ADT2 with M25202 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (ADT2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with AAA62363 of 10 ug. Western blot was performed from the immunoprecipitate using AAA62363 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: THP1 cells membrane fraction.Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
WB (Western Blot) (All lanes : ADT2 Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 33 KDaObserved band size : 33 KDa Blocking/Dilution buffer : 1x TBST.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (AAA126942).Overlay histogram showing K562 cells stained with AAA126942 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (AAA126942, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of BAG2 using anti-BAG2 antibody (AAA126942).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (#AAA126942) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.)
Application Data (Published customer image: T lymphocytes in PBLs following DPV gC DNA vaccination. 3, 5, 7, 14, 28, 42 days after vaccination, the isolated PBLs were stained with monoclonal antibodies against duck CD4 (A), and CD8 (B). The results presented are the mean of all specimens of each group +/- SD.From: Lian B, Cheng A, Wang M, Zhu D, Luo Q, Jia R, Liu F, Han X, Chen X. Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C. Virol J. 2011 May 10;8:214.)
Application Data (Published customer image:T lymphocytes in PBL following DEV UL24 DNA vaccination. 1, 2, 3, 4, 6, 8weeks after vaccination, the isolated PBL were stained with monoclonal antibodies against duck CD4 (A) and CD8 (B). The results presented are the mean of all specimens of each group. Statistical significance was determined using a student'st-test.From: Yu X, Jia R, Huang J, Shu B, Zhu D, Liu Q, Gao X, Lin M, Yin Z, Wang M, Chen S, Wang Y, Chen X, Cheng A. Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge. Vet Res. 2012 Jul 6;43:56.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant CRYAB protein using CRYAB antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CRYAB protein in paraffin embedded Carcinoma of Human thyroid tissue using CRYAB antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CRYAB protein (Right) or empty vector (Left) detected with CRYAB antibody)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7369, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7369, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human HVEM protein, His tagged protein PME100273 can bind Rabbit anti-HVEM monoclonal antibody (clone: DM131) in a linear range of 0. 1-12 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human HVEM protein, His tagged protein PME100273 can bind Rabbit anti-HVEM monoclonal antibody (clone: DM131) in a linear range of 0. 1-12 ng/ml.)
WB (Western Blot) (Western blot analysis of GRP78 BiP expression in (1) HeLa cell lysate; (2) C6 cell lysate (AAA47009).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA5)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human testis, using GRP78 BiP Antibody(AAA47009)HSPA5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA5 Antibody (AAA47009)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen, using LEF1 Antibody(AAA46967)LEF1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LEF1 Antibody (AAA46967)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of LEF1 expression in Jurkat cell lysate (AAA46967).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LEF1.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Western Blot
Pricing
WB (Western Blot) (The lysate of 293T (60ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ITLN1 antibody (1:250-1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of FLIP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FLIP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: K562 cell lysate)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)
WB (Western Blot) (Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.)
IF (Immunofluorescence) (ICC/IF analysis of S100A4 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody (1:500) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : A549 cell lysate Lane 3. : NIH/3T3 cell lysate Lane 4. : 3T3-L1 cell lysate)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUMB protein using NUMB antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUMB protein (Right) or empty vector (Left) detected with NUMB antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NUMB protein (red) or empty vector (blue) stained using NUMB antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of PLK1 protein in paraffin embedded Human liver tissue using PLK1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PLK1 protein (Right) or empty vector (Left) detected with PLK1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant PLK1 protein (red) or empty vector (blue) stained using PLK1 antibody)
ICC (Immunocytochemistry) (ICC staining Phospho-AMPK alpha 2 (S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-AMPK alpha 2 (S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200)
IF (Immunofluorescence) (IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc))
IP (Immunoprecipitation) (IP antibody use: 3ug DYKDDDDK Mouse IgG1 per ml Lysate, WB 1: 50001)Untransfected 293 cell lysate2)Transfected 293 cell lysate with Myc-Tag fusion protein3)IP (transfected 293+ normal Mouse IgG+Protein G agarose)4)IP (transfected 293+anti-C-Myc m)
ICC (Immunocytochemistry) (ICC staining AKR7A2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of AKR7A2 on human spermatozoa lysate using anti-AKR7A2 antibody at 1/1, 000 dilution. )
IHC (Immunohistochemisry) (Immunohistochemistry of Anterior Gradient 2 in paraffin-embedded colon using Anterior Gradient 2 Rabbit mAb at dilution 1/100)
IF (Immunofluorescence) (Immunofluorescence of Anterior Gradient 2 (green) in MCF-7 using Anterior Gradient 2 antibody at dilution 1/2, and DAPI(blue))
WB (Western Blot) (Western blot detection of Anterior Gradient 2 in K562,C6 cell lysates using Anterior Gradient 2 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:18kDa.)
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.