Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ICC (Immunocytochemistry) (ICC staining Musashi 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Musashi 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Musashi 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
WB (Western Blot) (Western blot analysis of Ki67 on HepG2 cell lysates using anti-Ki67 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining RPS3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RPS3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of RPS3 on PC12 cell lysates using anti-RPS3 antibody at 1/500 dilution.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Nudel on rat brain tissue lysate using anti-Nudel antibody at 1/2, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical of HDAC9 in Human tonsil tissue using HDAC9 antibody at dilution 1/20)
WB (Western Blot) (Western blot detection of HDAC9 in A549,HL-60 cell lysates using HDAC9 Rabbit mAb(1:1000 diluted).Predicted band size:111kDa.Observed band size:150kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of TXNRD2 in paraffin-embedded Human Cholangiocarcinoma using TXNRD2 Rabbit mAb at dilution 1/20)
WB (Western Blot) (Western blot detection of TXNRD2 in K562,Hela cell lysates using TXNRD2 Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:57kDa.)
Application Data (Pietilä. E.A.. Gonzalez-Molina. J.. Moyano-Galceran. L. et al. Co-evolution of matrisome and adaptive adhesion dynamics drives ovarian cancer chemoresistance. Nat Commun 12. 3904 (2021). h)
Application Data (Li. J.. Li. Y.. Zhou. X. et al. Upregulation of IL-15 in the placenta alters trophoblasts behavior contributing to gestational diabetes mellitus. Cell Biosci 11. 33 (2021). )
Application Data (Jukonen. J.. Moyano-Galceran. L.. Höpfner. K. et al. Aggressive and recurrent ovarian cancers upregulate ephrinA5. a non-canonical effector of EphA2 signaling duality. Sci Rep11. 8856 (2021).)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) Mouse Kidney. 3) Mouse Brain. diluted at 1:2000.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using ASK 1 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using ASK 1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) with DAB staining using ASK 1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ASK 1 Monoclonal Antibody against recombinant protein.)
IF (Immunofluorescence) (ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HepG2 cell lysate Lane 3.: SW480 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: K562 cell lysate Lane 6.: LnCap cell lysate Lane 7.: HeLa cell lysate Lane 8.: PC3 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of LRPAP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LRPAP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The lysate of Mouse kidney (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of Leptin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Leptin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: Leptin transfected 293T cell lysate)
WB (Western Blot) (The cell lysate(35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCN5L2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: HeLa cell lysate;)
ICC (Immunocytochemistry) (ICC/IF analysis of GCN5L2 in HeLa cells. The cell was stained (1:500). The secondary antibody (green) was used Alexa Fluor 488.)
IF (Immunofluorescence) (ICC/IF analysis of MAPK3 in HeLa cells line, stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with goat anti-mouse IgG-Texas Red conjugate (Red). ICC/IF analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MAPK1 recombinant protein Lane 2.: MAPK3 recombinant protein The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate)
IHC (Immunohistochemistry) (Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine(DAB).)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ATGA0326 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
IF (Immunofluorescence) (ICC/IF analysis of CD46 in HeLa cells. The cell was stained with AAA48143 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot) (The cell lysates of Ramos, HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot) (Western blot analysis of HSP70 expression in (1) K562 cell lysate; (2) 293T cell lysate (AAA47005).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA1A)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody (AAA47005)HSPA1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA1A Antibody (AAA47005)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Western Blot, Immunoprecipitation, Flow Cytometry, Immunohistochemistry
Pricing
Application Data (IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody AAA47760 is shown as AF4. Anti-GST antibodies served as negative controls. Positive control: K562 cell lysates.Optimal dilutions/concentrations should be determined by the end user.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HEXIM1 mAb. [Lot No. 2416C2a-1])
WB (Western Blot) (Detection of human HEXIM1 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2416C2a-1]Predicted molecular weight: 40 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-HEXIM1 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACO2 protein using ACO2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACO2 protein (Right) or empty vector (Left) detected with ACO2 antibody)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.)
WB (Western Blot) (Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CDC25A in human tonsil tissue using CDC25A antibody (Clone: ABM4A46) at 5 ug/ml.)
WB (Western Blot) (Expression analysis of CDC25A. Anti-CDC25A antibody (Clone: ABM4A46) was tested at 0.5 ug/ml and 2 ug/ml on (1) recombinant protein and (2) human heart lysates. respectively.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NSMCE2 protein using NSMCE2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NSMCE2 protein (Right) or empty vector (Left) detected with NSMCE2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NSMCE2 protein (red) or empty vector (blue) stained using NSMCE2 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of NT5DC1 protein in paraffin embedded Adenocarcinoma of Human colon tissue using NT5DC1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NT5DC1 protein (Right) or empty vector (Left) detected with NT5DC1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAGEB18 protein (Right) or empty vector (Left) detected with MAGEB18 antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of MAGEB18 protein in paraffin embedded Human tonsil tissue using MAGEB18 antibody)
MAGEB18 antibody was purified by affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry) (Staining of mouse platelets with CD61 antibody (FITC) and 0.125 ug of Rat IgG1K isotype Contol (PE) (left) or 0.06 ug of CD41 antibody (PE) (right). Total viable cells were used for analysis.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of SDS protein in paraffin embedded Carcinoma of Human lung tissue using SDS antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SDS protein (Right) or empty vector (Left) detected with SDS antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SDS protein (red) or empty vector (blue) stained using SDS antibody)
IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody.)
WB (Western Blot) (Western blot analysis of Akt(pan)1/2/3 expression in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.)
Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
IHC (Immunohistochemistry) (Anti-Amylin antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Application Data (Staining of human brain showing labelling of neurons with Mouse anti Human TGFbeta)
Application Data (Published customer imageGrowth factor expression in healing and non-union groups. Immunohistochemical analysis of TGF-beta, FGF-b, PDGF and BMP2/4 in healing and non-union groups at 1 and 3 weeks post-osteotomy. Images taken at x20 or x40 magnification and positive growth factor staining is seen as a red signal.From: Geris L, Reed AAC, Vander Sloten J, Simpson AHRW, Van Oosterwyck H (2010) Occurrence and Treatment of Bone Atrophic Non-Unions Investigated by an Integrative Approach. PLoS Comput Biol 6(9): e1000915.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.)
WB (Western Blot) (Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
FCM/FACS (Flow Cytometry) (HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECAM1 antibody, and then analyzed by flow cytometry.)
IF (Immunofluorescence) (Anti-PECAM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1.)
IHC (Immunohistochemistry) (Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity purified from tissue culture supernatant
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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