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SDS-PAGE (Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-BOB.1(ABT-BOB1)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-C-kit(ABT233)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK6 (ABT052)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Smad1/9(PTR2351) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MBP (ABT-MBP) antibody. The HRP-conjugated Goat anti-M)
IF (Immunofluorescence) (ICC/IF analysis of HSP60 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HSP60 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry) (Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot) (Western Blot analysis of Mouse,Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3C11 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).)
WB (Western Blot) (Western Blot analysis of Human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3C11 . Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
WB (Western Blot) (Western Blot analysis of Human Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3C11 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3C11 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000,1:5000 for 60 min at RT,5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RNF144B protein using RNF144B antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of RNF144B protein in paraffin embedded Human Kidney tissue using RNF144B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RNF144B protein (Right) or empty vector (Left) detected with RNF144B antibody)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD49f)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD49f:FITC)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD49f)
Application Data (Western blot analysis of Jurkat whole cell lysate probed with Mouse anti Human CD49f antibody followed by HRP conjugated Goat anti Human IgG, visualized using chemiluminescence)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD49f:RPE)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7365, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7365, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
WB (Western Blot) (Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.)
WB (Western Blot) (Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.)
WB (Western Blot) (Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of CYP1A2 protein in paraffin embedded Human thyroid tissue using CYP1A2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CYP1A2 protein (Right) or empty vector (Left) detected with CYP1A2 antibody)
Application Data (Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4).The blot was probed with anti-c-Cbl (AAA71500; lanes 1 & 2) or anti-c-Cbl (Tyr-700) ( ; lanes 3 & 4).)
WB (Western Blot) (Western blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.)
WB (Western Blot) (HAT-tag antibody (9A2F8)1 Western blot analysis of (1) 200 ng (2) 100 ng, (3) 50 ng and (4) 25 ng of a HAT-tag containing recombinant GGP1 protein with HAT-tag antibody at 0.5 ug/mL.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Acetyl P53(K382) Mouse mAb diluted at 1:2000)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl P53(K382) Mouse mAb diluted at 1:200.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using GSK3b Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3b Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using GSK3b Mouse mAb diluted at 1:1000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Acetyl NF kB P65 (K314/K315) Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl NF kB P65 (K314/K315) Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-GFAP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-GFAP antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GFAP on rat brain lysates using anti-GFAP antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti- Vitamin D Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- Vitamin D Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Vitamin D Receptor antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD62L (DREG56) FITC antibody (20 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry) (Separation of human neutrophil granulocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) FITC antibody (20 ul reagent / 100 ul of peripheral whole blood).)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with PDI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
WB (Western Blot) (Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Human liver)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Lamin A/C Monoclonal Antibody at dilution of 1:500.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Lamin A/C Monoclonal Antibody at dilution of 1:500.)
WB (Western Blot) (Western blot with Anti Lamin A/C Monoclonal Antibody at dilution of 1:3000. Lane 1: SH-SY5Y cell lysate, Lane 2: HL-60 cell lysate, Lane 3: U-87 MG cell lysate, Lane 4: Hep G2 cell lysate.)
WB (Western Blot) (Western blot analysis of KDEL expression in HeLa cell lysate (AAA124494).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDELR1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KDELR1)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver, using KDEL Antibody(AAA124494)KDELR1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KDELR1 Antibody (AAA124494)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U87 cells using anti-UBE3A antibody (AAA125906).Overlay histogram showing U87 cells stained with AAA125906 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- UBE3A Antibody (AAA125906, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of UBE3A using anti-UBE3A antibody (AAA125906).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: monkey COS-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- UBE3A antigen affinity purified monoclonal antibody (Catalog # AAA125906) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBE3A at approximately 100KD. The expected band size for UBE3A is at 100KD.)
Application Data (Published customer image:Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR -, red: SLA-DR+) in various body compartments of control and APP-infected pigs.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.)
Application Data (Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC)
Application Data (Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163)
Application Data (Published customer image:Representative pictures of immunohistochemical detection of CD163+ cells in tracheobronchial lymph node and spleen. CD163+ cells were detected in tracheobronchial lymph nodes from control (A) and APP-infected pigs (B) and in spleen from control (C) and APP-infected pigs (D). Immunohistochemical visualization: horseradish peroxidase, brown substrate, hematoxylin counterstain; c, cortex; ca, central artery; e, ellipsoid; f, follicle; mz, marginal zone; pals, periarterial lymphatic sheath, rp, red pulp; s, subcapsular sinus.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data (Staining of thrombin activated platelets with Mouse anti Human CD62P)
Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free (AAA50221))
Application Data (Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC)
Application Data (Staining of human peripheral blood platelets with Mouse anti Human CD62P)
Application Data (Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin))
FCM/FACS (Flow Cytometry) (Published customer image:Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) used for the evaluation of rabbit lymphoblastoid phenotype by flow cytometry.Image caption:Lymphoblastoid cell lines (LCLs) phenotypic analysis.LCLs were propagated from peripheral blood mononuclear cells of rabbits developing MCF after infection with Delta-GE or Delta-GE-rev viruses in Iscove’s modified Dulbecco’s medium (IMDM) containing 10% FCS and supplemented with recombinant human interleukin 2 (Roche, 10 IU/mL). Cells were maintained in medium replaced every 3–4 days and analyzed by flow cytometry for IgM+ B cells, CD8+ T cells and CD4+ T cells after 3 weeks culture (A).)
FCM/FACS (Flow Cytometry) (Published researcher image:Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) used to assess CD4 expression levels on rabbit mononuclear cells by flow cytometry.Image caption:Analysis of in vivo BrdU incorporation.Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody. In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry.A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis).B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P)
Application Data (Staining of rabbit peripheral blood lymphocytes with purified Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) followed by FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody)
FITC (Fluorescein Isothiocyanate) (Staining of rabbit peripheral blood lymphocytes with FITC conjugated Mouse anti Rabbit CD4 antibody, clone KEN-4)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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