Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IF (Immunofluorescence) (ICC/IF analysis of STOM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human STOM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: STOM Transfected 293T cell lysate)
FCM/FACS (Flow Cytometry) (Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot) (The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: Recombinant human TLR7 protein;)
WB (Western Blot) (Western Blot analysis using ABL1 antibodyWestern Blot showing ABL1 antibody used against truncated GST-ABL1 recombinant protein (1).)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PPP5C protein (Right) or empty vector (Left) detected with PPP5C antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of PPP5C protein in paraffin embedded Human ovary tissue using PPP5C antibody)
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of A431 cells using CF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control))
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of A431 cells using CF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using CaMKIIb/ g /&d (Phospho Thr287) Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using CaMKIIb/ g /&d (Phospho Thr287) (mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of CD3 epsilon in paraffin-embedded Human tonsil using CD3 epsilon Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of CD3 epsilon in Jurkat cell lysates using CD3 epsilon Rabbit mAb(1:500 diluted).Predicted band size:23kDa.Observed band size:23kDa.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Xanthine Oxidase on human kidney tissue lysate using anti-Xanthine Oxidase antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2, 000 dilution. Positive control: Lane 1: A431 Lane 2: A549 Lane 3: Rat brain Lane 4: Mouse brain)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- FATP2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-FATP2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FATP2 on HEK293 (1) and FATP2-hIgGFc transfected HEK293 (2) cell lysate using anti-FATP2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of FATP2 on human FATP2 recombinant protein using anti- FATP2 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Glucocorticoid Receptor (phospho-Ser226) in paraffin-embedded Human tonsil using Glucocorticoid Receptor (phospho-Ser226) Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of phospho-Glucocorticoid Receptor (Ser226) in Hela,A549,C6 cell lysates using phospho-Glucocorticoid Receptor (Ser226) Rabbit mAb(1:1000 diluted).Predicted band size:86kDa.Observed band size:94kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Lamin A/C in paraffin-embedded Human breast cancer tissue using Lamin A/C Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Lamin A/C in U251,Lncap,A549 using Lamin A/C Rabbit mAb(1:1000 diluted))
IHC (Immunohiostchemistry) (Immunohistochemistry of Optineurin in paraffin-embedded Human Cholangiocarcinoma using Optineurin Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Optineurin in Hela,A549,HL-60,U2OS,U87-MG cell lysates using Optineurin Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:75kDa.)
WB (Western Blot) (Western blot detection of IL8 in CHO-K1 transfected by IL8-PDGFR fusion protein cell lysate using IL8 mouse mAb (1:500(A)-1:1000(B) diluted).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).)
WB (Western Blot) (Western blot detection of hHR23b in K562.SW480.CHO-K1.3T3 and COS7 cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
WB (Western Blot) (Western blot detection of hHR23b in A431.K562.Jurkat.C6.3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
IHC (Immunohistochemistry) (Human kidney tissue was stained with Anti-Cytokeratin 18 (ABT054) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-Cytokeratin 18 (ABT054) Antibody)
IHC (Immunohiostchemistry) (Human colon carcinoma tissue was stained with Anti-Cytokeratin 18 (ABT054) Antibody)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK18 antibody at 1ug/ml. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
SDS-PAGE (Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-Myoglobin (ABT-MYG) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Beclin-1 Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using Beclin-1 Monoclonal Antibody at dilution of 1:200.)
WB (Western Blot) (Western Blot analysis of 1) 293T, 2) C2C12, 3) Rat brain using Beclin-1 Monoclonal Antibody at dilution of 1:2000.)
WB (Western Blot) (Western Blot: The extract of lung (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MFAP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohistochemistry) (Anti-MFAP4 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, AAA41001, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, AAA41001, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
WB (Western Blot) (Western blot of anti-DDR1 monoclonal antibody against truncated MBP-DDR1 recombinant protein(1).)
IHC (Immunohiostchemistry) (Anti-DDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry) (Anti-DDR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed Daudi cells immobilized on Shi-fixPLUS cover-slips. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).)
IF (Immunofluorescence) (alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.)
Application Data (Western blot analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate probed with Mouse anti Human CD19 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Application Data (Staining formalin-fixed, paraffin-embedded human tonsil with Mouse anti Human CD19)
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
MONOTOPE purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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