Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Immunohistochemistry of FUBP1 in paraffin-embedded Human colon cancer tissue using FUBP1 Rabbit mAb at dilution 1:100)
WB (Western Blot) (Western blot detection of FUBP1/FBP in K562,Rat Brain,Hela cell lysates using FUBP1/FBP Rabbit mAb(1:1000 diluted).Predicted band size:68kDa.Observed band size:74kDa.)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TH.)
IF (Immunofluorescence) (Anti-TH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TH.)
WB (Western Blot) (SaCas9 antibody (mAb) tested by Western blot. Lane 1: OSC Drosophila ovary cell lysate, transfected with SaCas9 (WT); Lane 2: OSC cell lysate transfected with SaCas9 (DN).)
Standard Curve (Sample) (Representative Standard Curve using mouse monoclonal anti-EpCAM (EM0421) for ELISA capture of human recombinant EpCAM extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
ICC (Immunocytochemistry) (Immunocytochemical labeling of EpCAM in aldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-EpCAM (EM0421). The antibody was detected using goat anti-mouse Ig:DyLight 594.)
WB (Western Blot) (Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LHX2 mAb. [Lot No.LHX2A12G1-2])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-LHX2 monoclonal antibody.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human NKp30 protein, hFc tagged protein PME100081 can bind Rabbit anti-NKp30 monoclonal antibody (clone: DM88) in a linear range of 1-100 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human NKp30 protein, hFc tagged protein PME100081 can bind Rabbit anti-NKp30 monoclonal antibody (clone: DM88) in a linear range of 1-100 ng/ml.)
Application Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag)
Application Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP)
Application Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 (AAA49256D800GA). Visualised using a LI-COR Odyssey infrared imaging system)
Application Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP)
WB (Western Blot) (Western blot analysis of PGC1 alpha expression in (1) HeLa cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate (AAA46914).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPARGC1A)
WB (Western Blot) (Detection of human CD31/PECAM-1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HEK293T, U2OS, Jurkat, A-549, GaMG, MCF-7, and HUVEC-C cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody (AAA213593 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation) (Detection of human CD31/PECAM-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody (AAA213593 lot 1) used for IP at 6 ul per reaction. CD31/PECAM-1 was also immunoprecipitated by rabbit anti-CD31/PECAM-1 antibody BL-1649A-3D8. For blotting immunoprecipitated CD31/PECAM-1, AAA213593 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemisry) (Detection of human CD31/PECAM-1 in FFPE placenta by immunohistochemistry. Antibody: Rabbit anti- CD31/PECAM-1 recombinant monoclonal antibody (AAA213593 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD31/PECAM-1 in FFPE THP-1 cells by immunocytochemistry. Antibody: Rabbit anti- CD31/PECAM-1 recombinant monoclonal antibody (AAA213593 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry) (Detection of human CD31 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD31 recombinant monoclonal (AAA213593 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STIM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STIM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PON2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PON2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-PON2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PON2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PON2 on A549 cell lysate using anti-PON2 antibody at 1/1, 000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ACE on human lung (1) and mouse kidney (2) cell lysate using anti-ACE antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human kidney tissue with DAB staining using ANP32A Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ANP32A Monoclonal Antibody against HeLa (1) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells using HXK I Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using HXK I Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded kidney tissues with DAB staining using HXK I Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using HXK I Monoclonal Antibody against Jurkat (1). HeLa (2). HepG2 (3) and NIH/3T3 (4) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using WIF-1 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) with DAB staining using WIF-1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using WIF-1 Monoclonal Antibody against HeLa (1). NIH/3T3 (2) and NTERA-2 (3) cell lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of human-liver-cancer tissue. 1.CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1.CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of Hela. diluted at 1:2000.)
SDS-PAGE (Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD141 (ABT145) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD63 (ABT-CD63) antibody. The HRP-conjugated Goat anti-M)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CHD6 mAb. [Lot No. 2174C2a-1])
WB (Western Blot) (Detection of human CHD6 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2174C2a-1]Predicted molecular weight: 305 kDa)
Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-CHD6 monoclonal antibody.)
WB (Western Blot) (Western Blot using anti-FOXP1 antibody RAB-S37 (AAA72222). Human skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-S37 at 0.003ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-FOXP1 antibody RAB-S37 (AAA72222). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of RAB-S37 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.