Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.)

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Heme Oxygenase 1 (HO-1), Monoclonal Antibody (Cat# AAA312174)

Full Name

Heme Oxygenase 1 (HO-1) Antibody

Gene Names

HMOX1; HO-1; HSP32; HMOX1D; bK286B10

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $470/0.1 mL $920/2x0.1 mL $1,340/3x0.1 mL $1,735/4x0.1 mL $1,940/5x0.1 mL

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WB (Western Blot)

IF (Immunofluorescence)

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TNF alpha, Monoclonal Antibody (Cat# AAA197037)

Full Name

Anti-Mouse TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12)

Gene Names

Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

Reactivity

Mouse

Applications

Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation

Purity

>95% by SDS-PAGE and HPLC
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Pricing

$560/5 mg $1,395/25 mg $2,180/50 mg $3,110/100 mg $6,060/2x100 mg $8,855/3x100 mg $11,500/4x100 mg $13,740/5x100 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of Phospho-PKC alpha (Thr638) in paraffin-embedded human colon cancer tissue using Phospho-PKC alpha (Thr638) Rabbit mAb at dilution 1/100)

WB (Western Blot) (Western blot detection of Phospho-PKC alpha (Thr638) in K562,C6 cell lysates using Phospho-PKC alpha (Thr638) Rabbit mAb(1:1000 diluted).Predicted band size:77kDa.Observed band size:80kDa.)

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PKC alpha, Monoclonal Antibody (Cat# AAA314290)

Full Name

Phospho-PKC alpha (Thr638) Rabbit mAb

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using PAPP-A Mouse Monoclonal Antibody (PAPPA/2716) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis Purified PAPP-A Mouse Monoclonal Antibody (PAPPA/2716). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with PAPP-A Mouse Monoclonal Antibody (PAPPA/2716).)

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PAPP-A/Pappalysin-1, Monoclonal Antibody (Cat# AAA215122)

Full Name

PAPP-A/Pappalysin-1 (Marker of Atherosclerosis and Aneuploid Fetus)

Gene Names

PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL)

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IF (Immunofluorescence) (Immunofluorescent staining of PFA-fixed HeLa cells. HSP60 Monoclonal Antibody (AE-1) (CF488). Nuclei stained with RedDot (red).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (AE-1).)

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HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA215454)

Full Name

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Gene Names

HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data

IHC (Immunohistochemistry) (IHC staining of human colon cancer tissue. diluted at 1:200.)

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CK19, Monoclonal Antibody (Cat# AAA320417)

Full Name

CK19 Monoclonal Antibody (11F5)

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD8a (PTR1338) antibody. The HRP-conjugated Goat anti-M)

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CD8 a, Monoclonal Antibody (Cat# AAA320590)

Full Name

CD8 a (PTR1338) Antibody

Gene Names

CD8A; CD8; MAL; p32; Leu2

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Protein G

Pricing

$215/0.02 mL $290/0.05 mL $350/0.1 mL $575/0.2 mL $1,110/2x0.2 mL $1,620/3x0.2 mL $2,105/4x0.2 mL $2,325/5x0.2 mL

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Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Annexin A1 (ABT-ANXA1) antibody. The HRP-conjugated Goat anti-M)

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Annexin A1, Monoclonal Antibody (Cat# AAA320595)

Full Name

Annexin A1 (ABT-ANXA1) Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Protein G

Pricing

$215/0.02 mL $290/0.05 mL $350/0.1 mL $575/0.2 mL $1,110/2x0.2 mL $1,620/3x0.2 mL $2,105/4x0.2 mL $2,325/5x0.2 mL

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Application Data

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using C/EBP ? Monoclonal Antibody (blue) and negative control (red).)

WB (Western Blot) (Western Blot analysis using C/EBP ? Monoclonal Antibody against human C/EBP ? (AA: 161-338) recombinant protein.)

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C/EBP beta, Monoclonal Antibody (Cat# AAA319975)

Full Name

C/EBP beta Monoclonal Antibody

Gene Names

CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PPX Monoclonal Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer using PPX Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis using PPX Monoclonal Antibody against HeLa. 293. Jurkat cell lysate.)

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PPX, Monoclonal Antibody (Cat# AAA320276)

Full Name

PPX Monoclonal Antibody

Gene Names

PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4

Reactivity

Human, Mouse, Rat, Bovine, Dog, Zebrafish

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

FCM/FACS (Flow Cytometry) (Human peripheral blood granulocytes are stained with Anti-Human CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated)(filled gray histogram). Unstained  granulocytes (empty black histogram) are used as control.)

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CD31, Monoclonal Antibody (Cat# AAA174572)

Full Name

Anti-Human CD31 Monoclonal Antibody (Alexa Fluor 488 Conjugated)

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human

Applications

Flow Cytometry

Pricing

$170/20 Tests $285/100 Tests $365/2x100 Tests $1,620/10x100 Tests

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Application Data (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:FITC)

Application Data (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:Alexa Fluor 647)

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CD4, Monoclonal Antibody (Cat# AAA49596)

Full Name

MOUSE ANTI BOVINE CD4:FITC

Applications

Flow Cytometry

Pricing

$700/0.1 mg $1,365/2x0.1 mg $1,990/3x0.1 mg $2,585/4x0.1 mg $2,925/5x0.1 mg

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Application Data (Published customer image; CSF1R-transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R-mApple+ cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG-eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R-mApple embryos indicates that transgene expression is restricted to CD45+ (B, green), CSF1R+ (C, green) cells in the mesenchyme (red arrowheads) and not CD45+ cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 um. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R-mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45+ (D, green) and CSF1R+ (E, green), but are CD41/61- (F, green). Scale bars in D-F: 100 um. BV, blood vessel lumen. (G) Scattered eGFP+ cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 um. (H-J) Colocalization of eGFP+ cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 um. From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.)

Application Data (Staining of Chicken peripheral blood cells with Mouse anti Chicken CD41/CD61:RPE)

Application Data (Staining of Chicken peripheral blood cells with Mouse anti Chicken CD41/61: FITC)

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CD41/CD61, Monoclonal Antibody (Cat# AAA49654)

Full Name

MOUSE ANTI CHICKEN CD41/CD61:RPE

Applications

Flow Cytometry

Pricing

$785/100 Tests $1,525/2x100 Tests $2,230/3x100 Tests $2,895/4x100 Tests $3,295/5x100 Tests

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Application Data (Staining of mouse spleen with Rat anti Mouse CD22: Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD22:FITC)

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CD22, Monoclonal Antibody (Cat# AAA49493)

Full Name

RAT ANTI MOUSE CD22:RPE

Gene Names

Cd22; Lyb8; Lyb-8; A530093D23

Applications

Flow Cytometry

Pricing

$600/100 Tests $1,160/2x100 Tests $1,695/3x100 Tests $2,200/4x100 Tests $2,465/5x100 Tests

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IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells, using Cyclin B1 Antibody .)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody.)

WB (Western Blot) (Western blot analysis of Cyclin B1 expression in HeLa cell lysate.)

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Cyclin B1, Monoclonal Recombinant Antibody (Cat# AAA315227)

Full Name

Cyclin B1 Rabbit mAb

Gene Names

CCNB1; CCNB

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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FCM/FACS (Flow Cytometry)

IP (Immunoprecipitation)

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Cytokeratin 18 (K1C18), Monoclonal Antibody (Cat# AAA62444)

Full Name

Cytokeratin 18 (K1C18) Mouse mAb

Gene Names

KRT18; K18; CK-18; CYK18

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

$445/0.05 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL $2,820/5x0.1 mL

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IF (Immunofluorescence) (IF analysis of 293T cells transfected with a DYKDDDDK-tagged protein using DYKDDDDK-Tag (3B9) Mouse mAb at a 1:1000 dilution.)

WB (Western Blot) (Lane 1: 293T cell lysate transfected with DYKDDDDK-tag protein.Lane 2: Mock transfected 293T cell lysate. The antibody dilution is 1:100.)

WB (Western Blot) (Western blot analysis of over-erpressed DYKDDDDK-tagged protein in 293T cell lysate, using DYKDDD0K-tag (3B9) Mouse mAb. The antibody dilutions are 1:2000 (lane 1). 1:5000 (lane 2). 1:1000 (lane 3) and 1:20000 (lane 4). Each lane was loaded with 2 ug of cell lysate. Lane 5 is 2 ug of mock transfected celL lysate at a dilution of 1:5000.)

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DYKDDDDK-Tag, Monoclonal Antibody (Cat# AAA62295)

Full Name

DYKDDDDK-Tag (3B9) Mouse mAb

Reactivity

All

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Pricing

$285/0.05 mL $355/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,795/5x0.2 mL

ELISA (Figure 1.ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human GFAP(68-377) protein, His tagged protein can bind Rabbit anti-GFAP(68-377) monoclonal antibody(clone: DM216) in a linear range of 1-50 ng/ml.)

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GFAP, Monoclonal Antibody (Cat# AAA47528)

Full Name

Anti-GFAP(68-377) antibody (DM216), Rabbit mAb

Reactivity

Human

Applications

ELISA

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$315/0.01 mg $750/0.1 mg $1,930/0.5 mg $3,755/2x0.5 mg $5,490/3x0.5 mg $7,125/4x0.5 mg $8,360/5x0.5 mg

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WB (Western Blot) (Western blot analysis of ARG1 expression in Mouse liver lysate (AAA47024).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARG1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using ARG1 Antibody(AAA47024)ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (AAA47024)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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ARG1/Arginase 1, Monoclonal Antibody (Cat# AAA47024)

Full Name

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical of paraffin-embedded human breast cancer using AAA118239 at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemical of paraffin-embedded human spleen tissue using AAA118239 at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemical of paraffin-embedded human lung tissue using AAA118239 at dilution of 1:200)

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Protein S100-A9, Monoclonal Antibody (Cat# AAA118239)

Full Name

Mouse anti-human Protein S100-A9 Monoclonal Antibody

Gene Names

S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387

Reactivity

Human. Other species are not tested. Please decide the specificity by homology

Applications

Immunohistochemistry

Purity

>95%, Protein G Purified

Pricing

$280/0.05 mg $375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,680/5x0.1 mg

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IHC (Immunohistochemisry) (Figure 3. IHC analysis of CRM1 using anti-CRM1 antibody (M01180).CRM1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CRM1 Antibody (M01180) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 3. IHC analysis of CRM1 using anti-CRM1 antibody (M01180).CRM1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CRM1 Antibody (M01180) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat lung tissue lysates,Lane 3: mouse liver tissue lysates,Lane 4: mouse lung tissue lysates,Lane 5: Rabbit IgG,Lane 6: Marker 1113,Lane 7: human HepG2 whole cell lysates,Lane 8: human SMMC-7721 whole cell lysates,Lane 9: human Hela whole cell lysates,Lane 10: human JURKAT whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.")

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CRM1, Monoclonal Antibody (Cat# AAA125138)

Full Name

Anti-CRM1 Antibody (monoclonal, 5G3)

Gene Names

XPO1; emb; CRM1; exp1

Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of K562 cells using anti-IRBIT/AHCYL1 antibody (AAA126957).Overlay histogram showing K562 cells stained with AAA126957 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRBIT/AHCYL1 Antibody (AAA126957, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (AAA126957).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat pancreas tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IRBIT/AHCYL1 antigen affinity purified monoclonal antibody (#AAA126957) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 59 kDa. The expected band size for IRBIT/AHCYL1 is at 59 kDa.)

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IRBIT/AHCYL1, Monoclonal Antibody (Cat# AAA126957)

Full Name

Anti-IRBIT/AHCYL1 Antibody Picoband (monoclonal, 2E7D9)

Gene Names

AHCYL1; DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

SDS-PAGE (SDS-PAGE Analysis Purified DAXX Mouse Monoclonal Antibody (PCRP-DAXX-5G11). Confirmation of Purity and Integrity of Antibody.)

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DAXX, Monoclonal Antibody (Cat# AAA215588)

Full Name

DAXX (Transcriptional Corepressor) Mouse Monoclonal Antibody [Clone PCRP-DAXX-5G11]

Gene Names

DAXX; DAP6; EAP1; BING2

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence, Flow Cytometry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL)

IF (Immunofluorescence) (Immunofluorescence Analysis of PFA-fixed HeLa cells using FOSL2 Mouse Monoclonal Antibody (PCRP-FOSL2-1B1) followed by goat anti-mouse IgG-CF488 (green). Counterstain is phalloidin.)

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Fos-related antigen 2 (FRA-2)/FOS-like antigen 2 (FOSL2), Monoclonal Antibody (Cat# AAA215660)

Full Name

Fos-related antigen 2 (FRA-2)/FOS-like antigen 2 (FOSL2) Mouse Monoclonal Antibody [Clone PCRP-FOSL2-1B1]

Gene Names

FOSL2; FRA2

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Alexa Fluor 647)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28:RPE)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin)

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CD28, Monoclonal Antibody (Cat# AAA49432)

Full Name

RAT ANTI HUMAN CD28

Gene Names

CD28; Tp44

Applications

Flow Cytometry, Immunohistochemistry

Pricing

$575/0.2 mg $1,110/2x0.2 mg $1,620/3x0.2 mg $2,105/4x0.2 mg $2,360/5x0.2 mg

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IHC (Immunohiostchemistry) (Anti-ICAM-1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry) (Anti-ICAM-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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ICAM1 / CD54, Monoclonal Antibody (Cat# AAA51822)

Full Name

Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM1 / CD54

Gene Names

ICAM1; BB2; CD54; P3.58

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry

Purity

Protein G Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohiostchemistry) (Anti-TGF-Beta antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry) (Anti-TGF-Beta antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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TGF-Beta, Monoclonal Antibody (Cat# AAA51878)

Full Name

Mouse Monoclonal [clone TB21] (IgG1,k) to Human TGF-Beta

Reactivity

Human

Applications

Functional Assay, Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Protein G Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Lymph node Tissue; Primary Ab: 30ug/ml Mouse AntiHuman IL6 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Lung Tissue; Primary Ab: 30ug/ml Mouse AntiHuman IL6 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemisry) (DAB staining on IHCP;Sample: Human Spleen Tissue; Primary Ab: 30ug/ml Mouse AntiHuman IL6 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohiostchemistry) (DAB staining on IHCP;Sample: Human Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse AntiHuman IL6 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml Mouse AntiHuman IL6 Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

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Interleukin 6 (IL6), Monoclonal Antibody (Cat# AAA151463)

Full Name

Monoclonal Antibody to Interleukin 6 (IL6)

Gene Names

IL6; HGF; HSF; BSF2; IL-6; IFNB2

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$205/0.1 mL $240/0.2 mL $390/0.5 mL $465/1 mL $1,255/5 mL $2,450/2x5 mL $3,580/3x5 mL $4,645/4x5 mL $5,685/5x5 mL

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WB (Western Blot) (WRN antibody (mAb) tested by Western blot. Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).)

IHC (Immunohiostchemistry) (WRN antibody (mAb) tested by Immunohistochemistry. Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line).)

IF (Immunofluorescence) (WRN antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 ug/ml dilution.)

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WRN, Monoclonal Antibody (Cat# AAA59986)

Full Name

WRN antibody (mAb)(Clone 195C)

Gene Names

WRN; RECQ3; RECQL2; RECQL3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Protein G Chromatography

Pricing

$250/0.01 mg $705/0.1 mg $1,375/2x0.1 mg $2,005/3x0.1 mg $2,605/4x0.1 mg $3,015/5x0.1 mg

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FCM/FACS (Flow Cytometry)

WB (Western Blot)

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GRP75/HSPA9, Monoclonal Antibody (Cat# AAA62454)

Full Name

GRP75/HSPA9 Mouse mAb

Gene Names

HSPA9; CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m

Reactivity

Human

Applications

Immunoprecipitation, Flow Cytometry, Western Blot

Pricing

$445/0.05 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL $2,820/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of Poliovirus Receptor/PVR in paraffin-embedded Human Cholangiocarcinoma using Poliovirus Receptor/PVR Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of Poliovirus Receptor/PVR in Hela,A549,U2OS cell lysates using Poliovirus Receptor/PVR Rabbit mAb(1:1000 diluted).Predicted band size:45kDa.Observed band size:60-80kDa.)

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Poliovirus Receptor, Monoclonal Antibody (Cat# AAA314562)

Full Name

Poliovirus Receptor Rabbit mAb

Gene Names

PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemistry of RAB45 (green) in Hela using RAB45 antibody at dilution 1/20, and DAPI(blue))

WB (Western Blot) (Western blot detection of RAB45 in Jurkat cell lysates using RAB45 Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:83kDa.)

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Rab45, Monoclonal Antibody (Cat# AAA314445)

Full Name

Rab45 Rabbit mAb

Gene Names

RASEF; RAB45

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carrcinoma Tissue using IL-8 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using IL-8 Mouse mAb diluted at 1:200.)

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IL-8, Monoclonal Antibody (Cat# AAA320510)

Full Name

IL-8 Mouse Monoclonal Antibody (13F8)

Gene Names

IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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Application Data

SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MGMT (PTR2300) antibody. The HRP-conjugated Goat anti-M)

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MGMT, Monoclonal Antibody (Cat# AAA320576)

Full Name

MGMT (PTR2300) Antibody

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Protein G

Pricing

$215/0.02 mL $290/0.05 mL $350/0.1 mL $575/0.2 mL $1,110/2x0.2 mL $1,620/3x0.2 mL $2,105/4x0.2 mL $2,325/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TFIIB Monoclonal Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon using TFIIB Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis using TFIIB Monoclonal Antibody against Jurkat. MCF7. HeLa cell lysate.)

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TFIIB, Monoclonal Antibody (Cat# AAA320285)

Full Name

TFIIB Monoclonal Antibody

Gene Names

GTF2B; TF2B; TFIIB

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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Application Data

FCM/FACS (Flow Cytometry) (Flow Cytometry analysis of HeLa cells stained with SMC1A (N-terminus)(red. 1/100 dilution). followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control. normal mouse IgG.)

IHC (Immunohiostchemistry) (IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) mouse mAb diluted 1/500-1/1000.)

WB (Western Blot) (Western blot detection of SMC1A(N-terminus) in HelaNE.Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.)

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SMC1A, Monoclonal Antibody (Cat# AAA320306)

Full Name

SMC1A (N-term) Mouse mAb

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain hippocampus (A). lung cancer (B). brain tumor (C). breast cance (D). showing cytoplasmic localization with DAB staining using Crystallin-?B Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis using Crystallin-?B Monoclonal Antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).)

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Crystallin-alphaB, Monoclonal Antibody (Cat# AAA320022)

Full Name

Crystallin-alphaB Monoclonal Antibody

Gene Names

CRYAB; CRYA2; CTPP2; HSPB5; CMD1II

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissueAll lanes: ATF5 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 31 kDaObserved band size: 35 kDa)

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ATF5, Monoclonal Recombinant Antibody (Cat# AAA243824)

Full Name

ATF5 Antibody

Gene Names

ATF5; ATFX; HMFN0395

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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ELISA (Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.)

WB (Western Blot) (AATF monoclonal antibody (M04), clone 3C7 Western blot of AATF expression in HeLa NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Anti-AATF antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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AATF, Monoclonal Antibody (Cat# AAA162294)

Full Name

Anti-AATF Antibody (clone 3C7)

Gene Names

AATF; DED; BFR2; CHE1; CHE-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Purified from ascites by Protein A

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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Application Data (Staining of mouse bone marrow with Rat anti Mouse CD34: Alexa Fluor 647)

Application Data (Staining of WEHI cells with Rat anti Mouse CD34: RPE)

Application Data (Staining of WEHI cells with Rat anti Mouse CD34)

Application Data (Staining of mouse bone marrow cells with Rat anti Mouse CD34:FITC)

Application Data (Staining of mouse bone marrow with Rat anti Mouse CD34: Alexa Fluor 488)

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CD34, Monoclonal Antibody (Cat# AAA49070)

Full Name

RAT ANTI MOUSE CD34:FITC

Gene Names

Cd34; AU040960

Applications

Flow Cytometry

Pricing

$500/0.1 mg $975/2x0.1 mg $1,430/3x0.1 mg $1,855/4x0.1 mg $2,040/5x0.1 mg

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IHC (Immunohiostchemistry) (Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 1.: K562 cell lysateLane 1.: MCF7 cell lysate)

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HMGB1, Monoclonal Antibody (Cat# AAA48035)

Full Name

HMGB1 antibody

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

$295/0.05 mL $380/0.1 mL $735/2x0.1 mL $1,010/5x0.05 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,400/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ATP citrate lyase expression in HeLa cell lysate (AAA47109).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACLY monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACLY)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody (AAA47109)ACLY was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACLY Antibody (AAA47109)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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ATP citrate lyase, Monoclonal Antibody (Cat# AAA47109)

Full Name

Anti-ATP citrate lyase Rabbit Monoclonal Antibody

Gene Names

ACLY; ACL; ATPCL; CLATP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western blot analysis of MMP8 expression in Human placenta lysate.)

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MMP8, Monoclonal Antibody (Cat# AAA47077)

Full Name

Anti-MMP8 Rabbit Monoclonal Antibody

Gene Names

MMP8; HNC; CLG1; MMP-8; PMNL-CL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BBX mAb. [Lot No. 2065C12a-1])

WB (Western Blot) (Detection of BBX by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2065C12a-1]Predicted molecular weight: 101 kDa)

Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-BBX monoclonal antibody.)

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BBX, Monoclonal Antibody (Cat# AAA36363)

Full Name

Mouse monoclonal antibody Anti-Human BBX

Gene Names

BBX; HBP2; ARTC1; MDS001; HSPC339

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STK3 mAb. [Lot No.3067C3a-1])

WB (Western Blot) (Detection of STK3 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3067C3a-1]Predicted molecular weight: 56 kDa)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-STK3 monoclonal antibody.)

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STK3, Monoclonal Antibody (Cat# AAA36517)

Full Name

Mouse monoclonal antibody Anti-Human STK3

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.