Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein-A
Pricing
Application Data (Figure 1. Strong collagen type IV specific staining of AAA77484 Clone 1043) in the basement membrane surrounding epithelial ducts and glomeruli in a frozen section of human kidney up to a 1:200 dilution. Also staining in the connective tissue and muscular layer of blood vessels. No staining of epithelial cells.)
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
Application Data (Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 Ab (ICO-115) (red) and isotype control Ab (green). PPI negative cell population was gated for analysis.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells stained with Mi2-? Monoclonal Antibody (red). followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control. normal mouse IgG.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Mi2-? Monoclonal Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon using Mi2-? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Mi2-? Monoclonal Antibody against Y7P. Raji cell lysate.)
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
WB (Western Blot) (Western blot analysis of CHAT expression in SH-SY5Y cell lysate (AAA47034).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHAT)
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
WB (Western Blot) (Western blot analysis of eIF5A expression in Jurkat cell lysate (AAA47076).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF5A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF5A)
WB (Western Blot) (Western blot analysis of SOX2 expression in (1) NCCIT cell lysate; (2) F9 cell lysate (AAA46874).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX2)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using SOX2 Antibody(AAA46874)SOX2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SOX2 Antibody (AAA46874)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SAPAP1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human liver cancer tissues with DAB staining using SAPAP1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using SAPAP1 Monoclonal Antibody against HEK293 (1) and GKAP-hIgGFc transfected HEK293 (2) cell lysate.)
WB (Western Blot) (Supt5H/SPT5 antibody (mAb) tested by Western blot 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
WB (Western Blot) (Supt5H/SPT5 antibody (mAb) tested by Immunoprecipitation 5 ug of Supt5H antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Supt5H from 300 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a the same Supt5H antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
ChIP (Chromatin Immunoprecipitation) (Supt5H/SPT5 antibody (mAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify Supt5H binding sites on chromosome 19.)
WB (Western Blot) (Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)
ICC (Immunocytochemistry) (Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Calnexin (CM4371), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight 594.)
IF (Immunofluorescence) (ICC/IF analysis of PSMD11 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: U87-MG cell lysate Lane 4.: NIH-3T3 cell lysate Lane 5.: PC3 cell lysate Lane 6.: TF1 cell lysate Lane 7.: MCF7 cell lysate)
ICC (Immunocytochemistry) (Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SATB2 mAb. [Lot No. SATBA4B10-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-SATB2 mAb. [Lot No. SATBA4B10-1]Predicted molecular weight: 82 kDa)
WB (Western Blot) (Detection of SATB2 by Western blot.Samples: Whole cell lysate from human HT1080 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. SATBA4B10-2]Predicted molecular weight: 82 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-SATB2 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant MOBKL2B protein using MOBKL2B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MOBKL2B protein (Right) or empty vector (Left) detected with MOBKL2B antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- A2M antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- A2M antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- A2M antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution.)
WB (Western Blot) (Figure-2: Western Blot analysis of MonkeyPox L1R Antibody: Anti-MonkeyPox L1R Antibody (Clone: ABM4F12.1B7) was used at 0.5 ug/ml on MonkeyPox L1R Recombinant protein.)
ELISA (Figure-1: An indirect ELISA is carried out by coating L1R antigen in serial dilution from 5ng/well to 0.625ng/well and purified Anti-L1R monoclonal antibodies ABM4F12.1B7 was added at 100ng/well. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1])
WB (Western Blot) (Detection of human RUVBL1 by Western blot.Samples: Whole cell lysate (50 ug) from HT-1080 cells. [Lot No. 2943C1a-1]Predicted molecular weight: 50 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody.)
WB (Western Blot) (Western blot analysis of anti-DRP-2 Monoclonal Antibody by NIH-3T3 cell line lysates. DRP-2 protein (arrow) was detected using the ascites Mab.)
WB (Western Blot) (Western blot of NIH-3T3 cell line lysates.)
IHC (Immunohiostchemistry) (Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry) (Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
WB (Western Blot) (Recombinant protein were subjected to SDS PAGE followed by western blot with AAA247960 (IL28A Antibody) at dilution of 1:5000)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA247960 (IL28A Antibody) at dilution of 1:50)
WB (Western Blot) (Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: PC12)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining ATG5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IRS1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IRS1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IRS1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IRS1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma(A). breast carcinoma(B). kidney cell carcinoma(C). bladder carcinoma tumor(D). showing membrane and cytoplasmic localization with DAB staining using IKK? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using IKK? Monoclonal Antibody against truncated IKK? recombinant protein (1).)
IF (Immunofluorescence) (Immunofluorescence of CDK2(green) in Hela cells using CDK2 Rabbit mAb at dilution 1:200, and DAPI(blue))
IHC (Immunohiostchemistry) (Immunohistochemistry of Cdk2 in paraffin-embedded Human tonsil using Cdk2 Rabbit mAb at dilution 1:100)
WB (Western Blot) (Western blot detection of CDK2 in Jurkat,C6,CHO-K1,Hela cell lysates using CDK2 Rabbit mAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:34kDa.)
WB (Western Blot) (Cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohistochemistry) (Anti-CBR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed 1G2 cells labeling ATP5C1 with M25352 at 1/200 dilution (blue) compared with a mouse monoclonal IgG isotype control (black). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation) (ATP5C1 was immunoprecipitated from 1mg of 1G2 cells membrane fraction, blotted with M25352 of 10 ug. Western blot was performed from the immunoprecipitate using M25352 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1:1G2 cells membrane fraction. Lane2: IP product of 1G2 cells membrane fraction.Predicted band size : 33 KDaObserved band size : 33 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot) (Western Blot Analysis of K562 and PC3 cell lysate using SERBP1 Mouse Monoclonal Antibody (SERBP1/3509).)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using SERBP1 Mouse Monoclonal Antibody (SERBP1/3509). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis Purified SERBP1 Mouse Monoclonal Antibody (SERBP1/3509). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with SERBP1 Mouse Monoclonal Antibody (SERBP1/3509).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with SERBP1 Mouse Monoclonal Antibody (SERBP1/3509).)
Application Data (Staining of red blood cells cells with Rat anti Human CD235a:Alexa Fluor 647)
Application Data (Staining of human peripheral blood erythrocytes with Rat anti Human CD235a:FITC)
Application Data (CD235a (Glycophorin A) - AAA49418 - Human hypophysis showing erythrocyte labelling in sinusoids. Formalin fixed, paraffin processed tissue)
Application Data (Staining of human peripheral blood erythrocytes with Rat anti Human CD235a:RPE)
IHC (Immunohiostchemistry) (Immunohistochemical of Tuberin in Human hepatic carcinoma tissue using Tuberin antibody at dilution 1/50)
WB (Western Blot) (Western blot detection of Tuberin in Hela,A549,MCF-7,293T cell lysates using Tuberin Rabbit mAb(1:1000 diluted).Predicted band size:201kDa.Observed band size:201kDa.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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