Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IP (Immunoprecipitation) (Immunoprecipitation of TYK2 transfected lysate using TYK2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TYK2 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TYK2 on HeLa cells using [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human colon using [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TYK2 expression in transfected 293T cell line using 1: TYK2 transfected lysate (133.7kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (TYK2 monoclonal antibody, Western Blot analysis of TYK2 expression in HeLa cells using .)

ELISA (Detection limit for recombinant GST tagged TYK2 is 1ng/mL using AAA25877 as a capture antibody.)

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TYK2, Monoclonal Antibody (Cat# AAA25877)

Full Name

TYK2 (Non-receptor Tyrosine-protein Kinase TYK2, JTK1) (PE)

Gene Names

TYK2; JTK1; IMD35

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TCEA3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (TCEA3 monoclonal antibody (M08), clone 4E11 Western Blot analysis of TCEA3 expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

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TCEA3, Monoclonal Antibody (Cat# AAA26209)

Full Name

TCEA3 (Transcription Elongation Factor A (SII), 3, TFIIS, TFIIS.H) (Biotin)

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin Rabbit mAb (AAA28537) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin Rabbit mAb (AAA28537) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Syntaxin Rabbit mAb (AAA28537) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Rat spinal cord, using Syntaxin Rabbit mAb (AAA28537) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of various lysates using Syntaxin Rabbit mAb (AAA28537) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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Syntaxin, Monoclonal Antibody (Cat# AAA28537)

Full Name

Syntaxin Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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FCM (Flow Cytometry) (Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)

IHC (Immunohistochemistry) (Immunohistochemistry analyis of SSTR2 in human cell line. SSTR2 was detected in immersion fixed human breast cancer cell line using AAA14651 (10ug/ml) for 3 hours at RT. Cells were stained with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown) and counterstained with DAPI (blue).)

IHC (Immunohistochemistry) (Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using AAA14651 (3ug/ml) overnight at 4°C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).)

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Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA14651)

Full Name

Somatostatin Receptor Type 2 (SSTr2)

Gene Names

SSTR2; SSTR2

Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified by Protein A affinity chromatography from hybridoma culture supernatant.

Pricing

$1,100/0.1 mg $2,115/2x0.1 mg $3,075/3x0.1 mg $3,980/4x0.1 mg $4,645/5x0.1 mg

WB (Western Blot) (WB (1:1000) analysis of recombinant protein JEV PreM with Anti-JEV PreM (AAA17973))

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JEV PreM, Monoclonal Antibody (Cat# AAA17973)

Full Name

JEV PreM Antibody

Applications

Western Blot

Pricing

$490/0.1 mg $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

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WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MUTYH, Monoclonal Antibody (Cat# AAA24577)

Full Name

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) APC

Gene Names

MUTYH; MYH

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

IHC (Immunohistochemistry) (Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Figure 2: Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).)

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CD133, Monoclonal Antibody (Cat# AAA17955)

Full Name

CD133 Antibody

Gene Names

PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$415/0.1 mg $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

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IF (Immunofluorescence) (Immunofluorescence of on HeLa cell using AAA24920 (10ug/ml).)

IHC (Immunohistochemistry) (Immunoperoxidase of formalin-fixed paraffin-embedded human placenta using AAA24920 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of PRPH expression in IMR-32 using AAA24920.)

Application Data (Detection limit for recombinant GST tagged PRPH is 1ng/ml using AAA24920 as a capture antibody.)

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PRPH, Monoclonal Antibody (Cat# AAA24920)

Full Name

PRPH (Peripherin, Neurofilament 4, NEF4, PRPH1) (Biotin)

Gene Names

PRPH; NEF4; PRPH1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)

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STIP1, Monoclonal Antibody (Cat# AAA26142)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (APC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (AAA28613) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37? for 24 hours or treated by nocodazole (50 ng/mL) at 37? for 20 hours or treated by CIP(20uL/400ul) at 37? for 1 hour. C2C12 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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POLR2A CTD-S5, Monoclonal Antibody (Cat# AAA28613)

Full Name

Phospho-POLR2A CTD-S5 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Testis Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Spinal cord Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Rat Colon Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Small intestine Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Pancreas Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: Rat Uterus Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Von Hippel Lindau Tumor Suppressor (vHL), Monoclonal Antibody (Cat# AAA21259)

Full Name

Monoclonal Antibody to Von Hippel Lindau Tumor Suppressor (vHL)

Gene Names

Vhl; Vhlh

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$265/0.1 mL $325/0.2 mL $525/0.5 mL $630/1 mL $1,675/5 mL $3,240/2x5 mL $4,720/3x5 mL $6,115/4x5 mL $7,465/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate (105.4 KDa).Lane 2: Non-transfected lysate.)

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NFKB1, Monoclonal Antibody (Cat# AAA26625)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (PE)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Published customer image: Mouse anti Pig IgM antibody, clone K52 1C3 used for thetection of IgM expressing cels in pig jejenum by immunofluorescence.Image caption:Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 um. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 um. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p)

Application Data (Published customer image: Mouse anti Pig IgM antibody, clone K52 1C3 used for the measurement of ova specific IgM levels in serum by ELISA.Image caption:OVA-specific antibody-mediated immune responses in serum from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n?=?5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. We measured serum anti-OVA IgM (A), IgA (B), IgG (C), IgG1 (D) and IgG2 (E) production on day 28, day 42 and day 49 after birth. Each data point represents an individual animal and median values are indicated by horizontal lines. *p?)

Application Data (Published customer image: Mouse anti Pig IgM antibody, clone K52 1C3 used for the measurement of ova specific IgM levels in lung lavages by ELISA.Image caption:OVA-specific antibody-mediated immune responses in lung washes from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n?=?5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. Lung lavages were collected four weeks post i.p. immunization and OVA-specific serum IgM (A), IgA (B), IgG (C), IgG1 (D), and IgG2 (E) titres were measured. ELISA titres are expressed as the reciprocal of the highest dilution resulting in a reading of two standard deviations above the negative control. Each data point represents an individual animal and median values are indicated by horizontal lines. *p?)

Application Data (Native pig IgM (5276-6504) detected with Mouse anti Pig IgM followed by Goat anti Mouse IgG:HRP)

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IgM, Monoclonal Antibody (Cat# AAA12250)

Full Name

MOUSE ANTI PIG IgM

Reactivity

Pig

Applications

Flow Cytometry, Immunohistochemistry

Pricing

$495/0.1 mg $945/2x0.1 mg $1,370/3x0.1 mg $1,760/4x0.1 mg $1,930/5x0.1 mg

SDS-PAGE (Analysis Purified CD90 Mouse Monoclonal antibody. Confirmation of Purity and Integrity of Antibody.)

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Cd90, Monoclonal Antibody (Cat# AAA13815)

Full Name

Cd90

Gene Names

THY1; CD90

Reactivity

Human, Monkey. Others not known.

Applications

Flow Cytometry, Immunofluorescence, Functional Assay

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed HeLa cells using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IF (Immunofluorescence) (Immunofluorescence staining of paraformaldehyde-fixed HeLa cells with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).)

SDS-PAGE (SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).)

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Annexin A1, Monoclonal Antibody (Cat# AAA23918)

Full Name

Annexin A1/(Hairy Cell Leukemia Marker)

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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SEC-HPLC (The purity of this product is >95% as determined by SEC-MALS.)

SDS-PAGE (SDS PAGE for Donanemab)

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Donanemab, Monoclonal Antibody (Cat# AAA18886)

Full Name

Research Grade Donanemab

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human

Purity

>95%, by SDS-PAGE; Protein A or G purified from cell culture supernatant.

Pricing

$510/0.1 mg $1,380/1 mg $2,665/2x1 mg $3,875/3x1 mg $5,020/4x1 mg $5,715/5x1 mg

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Application Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 750)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin)

Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Pacific Blue)

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CD14, Monoclonal Antibody (Cat# AAA12224)

Full Name

MOUSE ANTI HUMAN CD14:Biotin

Applications

Flow Cytometry

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

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WB (Western Blot) (Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (AAA23809 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat (10 ug), Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (AAA23809 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

Simple Western (Detection of human CTCF by Simple Western. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

SWB (Simple Western) (Detection of mouse CTCF by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation) (Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (AAA23809 lot 4) used for IP at 20 ul/mg lysate. CTCF was also immunoprecipitated by a previous lot of this antibody (AAA23809 lot 1) and a second antibody against CTCF . For blotting immunoprecipitated CTCF, AAA23809 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistchemistry) (Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse CTCF by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse CTCF (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CTCF (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (AAA23809) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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CTCF, Monoclonal Recombinant Antibody (Cat# AAA23809)

Full Name

Rabbit anti-CTCF Recombinant Monoclonal Antibody [BLR041F]

Gene Names

CTCF; MRD21

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

Purified

Pricing

$295/0.02 mL $480/0.1 mL $915/2x0.1 mL $980/5x0.02 mL $1,325/3x0.1 mL $1,705/4x0.1 mL $1,855/5x0.1 mL

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Application Data (Zika rash: arm.)

Application Data (Aedes aegypti Image source: Centers for Disease Control and Prevention Publich Health Image Library.)

ELISA (An ELISA plate was coated with 2ug/ml of NS1 antigen/well, then blocked with 5% BSA. AAA24113 was titrated as shown in the figure, starting from a concentration of 1ug/ml, and the detection antibody used was IgM (HRP) Goat anti-human. The substrate used was TMB (KPL).)

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Zika Virus NS1, Monoclonal Antibody (Cat# AAA24113)

Full Name

Human Anti-Zika Virus NS1 (Zika Virus Nonstructural Protein)

Applications

ELISA

Purity

>98% (SDS-PAGE). Purified from cell culture supernatant by lectin affinity chromatography.

Pricing

$1,815/0.1 mg $3,510/2x0.1 mg $5,120/3x0.1 mg $6,640/4x0.1 mg $7,875/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])

WB (Western Blot) (ROCK2 monoclonal antibody (M02), clone 1E12 Western Blot analysis of ROCK2 expression in Hela S3 NE.)

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ROCK2, Monoclonal Antibody (Cat# AAA26224)

Full Name

ROCK2 (Rho-Associated, coiled-coil Containing Protein Kinase 2, KIAA0619) (Biotin)

Gene Names

ROCK2; ROCK-II

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of HeLa celLauren Shoreusing AAA24076 (10ug/ml).)

Application Data (Detection limit for recombinant GST tagged UTRN is 0.1ng/ml using AAA24076 as a capture antibody.)

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Utrophin, Monoclonal Antibody (Cat# AAA24076)

Full Name

Utrophin (UTRN, Dystrophin-related Protein 1, DRP1, DRP-1, DMDL, DRP)

Gene Names

UTRN; DRP; DMDL; DRP1

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in NIH/3T3)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and HSPA1B mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1B is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1A, Monoclonal Antibody (Cat# AAA24832)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (Biotin)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in Raw 264.7.)

WB (Western Blot) (ADPGK monoclonal antibody, Western Blot analysis of ADPGK expression in HeLa.)

WB (Western Blot) (Western blot analysis of ADPGK over-expressed 293 cell line, cotransfected with ADPGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ADPGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ADPGK is 0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ADPGK expression in transfected 293T cell line by ADPGK monoclonal antibody. Lane 1: ADPGK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (34.03kD).)

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ADPGK, Monoclonal Antibody (Cat# AAA25600)

Full Name

ADPGK (ADP-dependent Glucokinase, ADP-GK, RbBP-35, PSEC0260, 2610017G09Rik, DKFZp434B195) (PE)

Gene Names

ADPGK; ADP-GK; 2610017G09Rik

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)

WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)

WB (Western Blot) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

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CDADC1, Monoclonal Antibody (Cat# AAA25345)

Full Name

CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (HRP)

Gene Names

CDADC1; NYD-SP15; bA103J18.1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EIF5 monoclonal antibody, Western Blot analysis of EIF5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged EIF5 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF5 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF5 expression in transfected 293T cell line by EIF5 monoclonal antibody. Lane 1: EIF5 transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.15kD).)

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EIF5, Monoclonal Antibody (Cat# AAA25089)

Full Name

EIF5 (Eukaryotic Translation Initiation Factor 5, eIF-5) (FITC)

Gene Names

EIF5; EIF-5; EIF-5A

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Figure 1. Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FACL4/ACSL4 antigen affinity purified monoclonal antibody (#AAA19713) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 68 kDa.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-FACL4/ACSL4 antibody (AAA19713).Overlay histogram showing HepG2 cells stained with AAA19713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FACL4/ACSL4 Antibody (AAA19713, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 6. IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (AAA19713).FACL4/ACSL4 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FACL4/ACSL4 Antibody (AAA19713) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

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FACL4/ACSL4, Monoclonal Antibody (Cat# AAA19713)

Full Name

Anti-FACL4/ACSL4 Antibody Picoband (monoclonal, 4I7)

Gene Names

ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Porcine Colon Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Kidney Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Lymph node Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Porcine Small intestine Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Adrenal gland Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Uterus Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human Placenta Tissue; Primary Ab: 30ug/ml Mouse Anti-human YWHAz Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), Monoclonal Antibody (Cat# AAA21256)

Full Name

Monoclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

Gene Names

YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$250/0.1 mL $315/0.2 mL $505/0.5 mL $610/1 mL $1,600/5 mL $3,085/2x5 mL $4,495/3x5 mL $5,825/4x5 mL $7,110/5x5 mL

SDS-PAGE (SDS PAGE for Leronlimab)

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Leronlimab, Monoclonal Antibody (Cat# AAA18889)

Full Name

Research Grade Leronlimab

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Purity

>95% by SDS-PAGE; Protein A or G purified from cell culture supernatant.

Pricing

$510/0.1 mg $1,380/1 mg $2,665/2x1 mg $3,875/3x1 mg $5,020/4x1 mg $5,715/5x1 mg

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IF (Immunofluorescence) (Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.)

FCM (Flow Cytometry) (Flow Cytometric Analysis of A549 cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

FCM (Flow Cytometry) (Flow Cytometric Analysis of T98G cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

WB (Western Blot) (Western Blot Analysis of HeLa cell lysate using S100A4 Mouse Monoclonal Antibody (S100A4/1482).)

SDS-PAGE (SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/1482). Confirmation of Integrity and Purity of Antibody.)

WB (Western Blot) (Western Blot of HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1482).)

IHC (Immunohistochemistry) (Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1482).)

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S100A4, Monoclonal Antibody (Cat# AAA23892)

Full Name

S100A4 (Marker of Tumor Metastasis)

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 ug) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human GITR/TNFRSF18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used for IP at 6 ul per reaction. GITR/TNFRSF18 was also immunoprecipitated by rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BL-1066-1D11]. For blotting immunoprecipitated GITR/TNFRSF18, AAA23817 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human GITR/TNFRSF18 by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human GITR/TNFRSF18 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal (AAA23817 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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GITR/TNFRSF18, Monoclonal Recombinant Antibody (Cat# AAA23817)

Full Name

Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [BLR068G]

Gene Names

TNFRSF18; AITR; GITR; CD357; GITR-D

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 750)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin)

Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Pacific Blue)

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CD14, Monoclonal Antibody (Cat# AAA12087)

Full Name

MOUSE ANTI HUMAN CD14:RPE

Applications

Flow Cytometry

Pricing

$325/25 Tests $605/2x25 Tests $865/3x25 Tests $1,115/4x25 Tests $1,125/5x25 Tests

Hazard Indicator

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COMPLEMENT FACTOR I, Monoclonal Antibody (Cat# AAA11964)

Full Name

MOUSE ANTI HUMAN COMPLEMENT FACTOR I

Gene Names

CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Western Blot, Functional Assay

Pricing

$860/0.1 mg $1,650/2x0.1 mg $2,395/3x0.1 mg $3,095/4x0.1 mg $3,535/5x0.1 mg

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Porcine Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-human AQP4 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine CerebeluM Tissue; Primary Ab: 30ug/ml Mouse Anti-human AQP4 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine Spinal cord Tissue; Primary Ab: 30ug/ml Mouse Anti-human AQP4 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Porcine CerebuM Tissue; Primary Ab: 30ug/ml Mouse Anti-human AQP4 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Glioma Tissue; Primary Ab: 20ug/ml Mouse Anti-human AQP4 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human CerebuM Tissue; Primary Ab: 20ug/ml Mouse Anti-human AQP4 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Aquaporin 4 (AQP4), Monoclonal Antibody (Cat# AAA21257)

Full Name

Monoclonal Antibody to Aquaporin 4 (AQP4)

Gene Names

AQP4; MIWC; HMIWC2

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $465/0.5 mL $560/1 mL $1,465/5 mL $2,835/2x5 mL $4,125/3x5 mL $5,345/4x5 mL $6,500/5x5 mL

WB (Western Blot) (Western blot (1:1000) analysis of recombinant protein LM Hly with LM Hly antibody)

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LLO, Monoclonal Antibody (Cat# AAA17939)

Full Name

LLO antibody

Reactivity

Recognizes recombinant protein LM Hly.

Applications

Western Blot

Pricing

$935/0.1 mg $1,800/2x0.1 mg $2,610/3x0.1 mg $3,380/4x0.1 mg $3,885/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA26624)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (PE)

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (77.26kD).)

IP (Immunoprecipitation) (Immunoprecipitation of STK38 transfected lysate using STK38 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK38 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK38 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue [antibody concentration 5ug/ml])

WB (Western Blot) (STK38 monoclonal antibody Western Blot analysis of STK38 expression in human kidney.)

WB (Western Blot) (Western Blot analysis of STK38 expression in transfected 293T cell line by STK38 monoclonal antibody Lane 1: STK38 transfected lysate (54.2kD). Lane 2: Non-transfected lysate.)

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STK38, Monoclonal Antibody (Cat# AAA25857)

Full Name

STK38 (Serine/Threonine-protein Kinase 38, NDR1 Protein Kinase, Nuclear Dbf2-related Kinase 1, NDR1) (PE)

Gene Names

STK38; NDR; NDR1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of mouse CD5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, EL4, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human CD5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MOLT-4, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human CD5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MOLT-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871 lot 1) used for IP at 20 ul/mg lysate. CD5 was also immunoprecipitated by a polyclonal antibody against the same eptiope of CD5 . For blotting immunoprecipitated CD5, AAA23871 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistchemistry) (Detection of mouse CD5 by immunohistochemistry. Sample: FFPE section of mouse thymus. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human CD5 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse CD5 by immunocytochemistry. Sample: FFPE section of EL4 cells. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human CD5 by immunocytochemistry. Sample: FFPE section of HH cells. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse CD5 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CD5 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD5 recombinant monoclonal antibody (AAA23871) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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CD5, Monoclonal Recombinant Antibody (Cat# AAA23871)

Full Name

Rabbit anti-CD5 Recombinant Monoclonal Antibody [BLR219K]

Gene Names

CD5; T1; LEU1

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (CD58 monoclonal antibody, Western Blot analysis of CD58 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CD58 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CD58 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 2ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human lymphoma tissue [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (52.14kD).)

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CD58, Monoclonal Antibody (Cat# AAA25344)

Full Name

CD58 (Lymphocyte Function-associated Antigen 3, Ag3, Surface Glycoprotein LFA-3, LFA3) (HRP)

Gene Names

CD58; ag3; LFA3; LFA-3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.