Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using G6PD Monoclonal Antibody (green) and negative control (red).)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded ovarian cancer tissues with DAB staining using G6PD Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using G6PD Monoclonal Antibody against HeLa (1). MCF-7 (2). Jurkat (3) and K562 (4) cell lysate.)
IF (Immunofluorescence) (ICC/IF analysis of PDIA3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDIA3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDIA3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : NIH3T3 cell lysate Lane 4. : Raw 264.7 cell lysate Lane 5. : LNCaP cell lysate)
IF (Immunofluorescence) (Immunofluorescencestainingofhumanperipheralbloodmonocyteswithanti-CD11a(AAA72514)VIPIIIB1 ImmunofluorescenceanalysisofparaformaldehydefixedhumanperipheralbloodmonocytesonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofVIPIIIB1(AAA72514)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72514,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence) (Immunofluorescencestainingofhumanperipheralbloodmonocyteswithanti-CD11a(AAA72514)VIPIIIB1 ImmunofluorescenceanalysisofparaformaldehydefixedhumanperipheralbloodmonocytesonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofVIPIIIB1(AAA72514)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72514,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot) (Detection of human CRSP7 by Western blot.Samples: Whole cell lysate (25 ug) from HEK293 cells. [Lot No. 2216C1a-1]Predicted molecular weight: 65 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-CRSP7 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse mAb diluted at 1:200.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Bax Mouse mAb (AAA320513) diluted at 1:200 (4 degree overnight). 2. High-pressure and temperature Citric acid. pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot) (Western blot detection of Bax in human breast cancer cell line MCF-7(A). MDA-MB-231(B) and Cal51 (C) using Bax mouse mAb (AAA320513. 1:1000 diluted).Predicted band size: 20kDa.Observed band size:20kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
SDS-PAGE (Whole cell lysates of LnCap were separated by 8% SDS-PAGE, and the membrane was blotted with anti-PSMA antibody. The HRP-conjugated)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PMSA antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human TNFRSF10B protein, mFc tagged protein PME100465 can bind Rabbit anti-TNFRSF10B monoclonal antibody (clone: DM114) in a linear range of 0.6-90 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human TNFRSF10B protein, mFc tagged protein PME100465 can bind Rabbit anti-TNFRSF10B monoclonal antibody (clone: DM114) in a linear range of 0.6-90 ng/ml.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Lung CarcinomaTissue using HSP40 Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon CarcinomaTissue using HSP40 Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Hela Cell. 2) Mouse Liver. 3) PC12 Cell Lysate using HSP40Mouse Monoclonal mAb diluted at 1:2.000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human KidneyTissue using ERK5 Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Hela Cell. 2) PC12 Cell. 3)3T3 Cell Lysate using ERK5Mouse Monoclonal Antibody diluted at 1:2.000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of extracts from Hela cells. untreated (-) or treated with TSA (1 uM. 18 hr; +). using Acetyl- a-tubulin(Lys40) Mouse mAb 1:2000.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
WB (Western Blot) (Western Blot analysis of Arabidopsis using Rubisco (Large Chain) Monoclonal Antibody at dilution of 1)1:2000 2)1:5000. Observed MW: 53kDa)
WB (Western Blot) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LNX1.)
WB (Western Blot) (Detection of human TRIOBP by Western blot.Samples: Whole cell lysate (25 ug) from HEK293 cells. [Lot No. 2438C1a-1]Predicted molecular weight: 74 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-TRIOBP monoclonal antibody.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of A549 cells using anti-Calpain 1 antibody (AAA125919).Overlay histogram showing A549 cells stained with AAA125919 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calpain 1 Antibody (AAA125919, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 2. IF analysis of Calpain 1 using anti- Calpain 1 antibody (AAA125919).Calpain 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Calpain 1 Antibody (AAA125919) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of Calpain 1 using anti-Calpain 1 antibody (AAA125919).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Calpain 1 antigen affinity purified monoclonal antibody (Catalog # AAA125919) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD.)
Application Data (Formalin-fixed, paraffin-embedded human histiocytoma stained with TNF alpha Mouse Monoclonal Antibody (SPM543).)
IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells using TNF alpha Mouse Monoclonal Antibody (SPM543) followed by goat anti-mouse IgG-CF488 (green). Counterstain is RedDot.)
WB (Western Blot) (Western blot analysis of CD34 in expression NIH/3T3 cell lysate (AAA46999).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD34)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using CD34 Antibody(AAA46999)CD34 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD34 Antibody (AAA46999)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis on (1)A431 cell lysate; (2)C6 cell lysate using alpha-SMA antibody (AAA47019).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTA2)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody(AAA47019)ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (AAA47019)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-CD31 antibody BAG-85D10 . Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody or the rabbit IgG version of BAG-85D10 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of fixed K562 cells with anti-CD31 antibody BAG-85D10 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BAG-85D10 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX3X mAb. [Lot No. 2253C5a-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX3X mAb. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)
WB (Western Blot) (Detection of DDX3X by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-DDX3X monoclonal antibody.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Detection of human CD22 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, Daudi, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CD22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used for IP at 12 ul/mg lysate. CD22 was also immunoprecipitated by a second antibody against a different epitope of CD22 (BL-2551B-1F2). For blotting immunoprecipitated CD22, AAA213644 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry) (Detection of human CD22 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human CD22 by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry) (Detection of human CD22 (shaded) in Raji cells by flow cytometry. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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