Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Fig: 2 Immunohistochemical analysis of CD31 in human lung squamous cell carcinoma (left) and bladder transitional cell carcinoma (right) using CD31 antibody (Clone: PECAM-1) at 1: 200 dilution.)
FCM/FACS (Flow Cytometry) (Fig.1: FLOW Cytometry analysis of CD31 in human PBMC using 0.5ug antibody per 10^6 cells. Green represents isotope control. Red represents anti-CD31 antibody (Clone: PECAM-1). Goat anti-mouse IgG conjugated with PE was used as secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of PYCR2 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using PYCR2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PYCR2 protein (Right) or empty vector (Left) detected with PYCR2 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PYCR2 protein using PYCR2 antibody)
Application Data (Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and Rat IgG2b isotype control detected with Goat F(ab')2 anti Rat IgG:RPE . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 and Rat anti Human CD45 detected with Goat F(ab')2 anti Rat IgG:PE . All experiments performed on human peripheral blood gated on live single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data (Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Rat IgG2b isotype control detected with Goat F(ab')2 anti Rat IgG:FITC . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Rat anti Human CD45 detected with Goat F(ab')2 anti Rat IgG:FITC . All experiments performed on human peripheral blood gated on live single cell lymphocytes in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data (Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PRPF8 mAb. [Lot No. 2834C1a-1])
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PRPF8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2834C1a-1])
WB (Western Blot) (Detection of PRPF8 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2834C1a-1]Predicted molecular weight: 273 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-PRPF8 monoclonal antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical staining of rat spleen using anti-CD28 antibody Formalin fixed rat spleen slices were were stained at 3 ug/ml.)
WB (Western Blot) (Western Blot using anti-CD28 antibody Rat thymus (lane 1), rat lymph node (lane 2), rat thyroid (lane 3) and rat spleen (lane 4) sample were resolved on a 12% SDS PAGE gel and blots probed at 3 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining Lipoprotein a (green) and actin filaments (red) in HepG2 cell. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Lipoprotein a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Lipoprotein a antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Lipoprotein a antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Lipoprotein a on human Lipoprotein a recombinant protein using anti- Lipoprotein a antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of A549 cells using DDR2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal musle tissues with AEC staining using DDR2 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using DDR2 Monoclonal Antibody against truncated DDR2 recombinant protein.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human stomach cancer tissues with DAB staining using PGA5 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using PGA5 Monoclonal Antibody against HepG2 (1) and SMMC-7721 (2) cell lysate.)
SDS-PAGE (Hela whole cell lysates were separated by 6% SDS-PAGE, and the membrane was blotted with anti-MDR1(ABT252) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-G3BP-1 (PTR1324) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD117(ABT064) antibody. The HRP-conjugated Goat anti-M)
IF (Immunofluorescence) (ICC/IF analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : HeLa cell lysate Lane 3 : A431 cell lysate Lane 4 : A549 cell lysate Lane 5 : Jurkat cell lysate Lane 6 : MCF7 cell lysate Lane 7 : PC3 cell lysate)
IF (Immunofluorescence) (Immunofluorescent analysis of A431 cells, using Collagen XVII Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin, using Collagen XVII Antibody(AAA47126)COL17A1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COL17A1 Antibody (AAA47126)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of Collagen XVII expression in Human skin lysate (AAA47126).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COL17A1)
FCM/FACS (Flow Cytometry) (Intracellular flow analysis of hTLR8 in U937 using 2 ug/10^6 cells of hTLR8 antibody (Clone: ABM15F6). Green represents isotype control; red represents anti-hTLR8 antibody. Goat anti-mouse PE conjugate was used as secondary.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of TLR8 in small cell carcinoma of esophagus using TLR8 antibody (Clone: ABM15F6) at 5 ug/ml.)
Application Data (Western blot analysis of K562 cell lysate using 0.5mg/mL mouse anti-PML mAb clone B3E9. The band shows on the blot is the 106KDa chimeric PML/RARa protein.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of PPME1 protein in paraffin embedded Human ovary tissue using PPME1 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PPME1 protein (Right) or empty vector (Left) detected with PPME1 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PPME1 protein using PPME1 antibody)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Standard Curve (Sample) (Representative Standard Curve using mouse monoclonal anti-EpCAM (EM0421) for ELISA capture of human recombinant EpCAM extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
ICC (Immunocytochemistry) (Immunocytochemical labeling of EpCAM in aldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-EpCAM (EM0421). The antibody was detected using goat anti-mouse Ig:DyLight 594.)
WB (Western Blot) (Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LHX2 mAb. [Lot No.LHX2A12G1-2])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-LHX2 monoclonal antibody.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysateAll lanes: MMP12 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 55 kDaObserved band size: 54, 45 kDa)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
ICC (Immunocytochemistry) (ICC staining Laminnin 5 alpha 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Laminnin 5 alpha 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of Laminnin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-appendix tissue. 1.Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1.Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of lysates from 1) Hela. 2) Mouse Heart tissue. 3) Rat Heart Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) Mouse Heart tissue. 3) Rat Heart Tissue. diluted at 1:2000.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast tissue. 1.IDE Monoclonal Antibody(3H4)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1.IDE Monoclonal Antibody(3H4) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) HepG2. diluted at 1:2000)
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
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What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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