Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Figure 2: Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution.; 1. hACE2 (Fc protein).; 2. hACE2 (Ecto domain) (DYKDDDDK-tagged).; 3. Other hGITR (Fc protein) (control).)
FCM/FACS (Flow Cytometry) (Figure 1: FACS: Cell Surface staining of human ACE2 on HepG2 cell line. Red: Human anti-ACE2 antibody (Clone:AC18F), 1ug/10^6 cells were used. Green : Isotype control, mouse IgG1, 1ug/ 10^6 cells was used as control. PE conjugated Goat anti-mouse IgG was used as secondary antibody.)
FCM/FACS (Flow Cytometry) (BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Mouse Anti-Rat IgG2b-BIOT and Streptavidin-FITC)
ELISA (ELISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2b-BIOT followed by Streptavidin-HRP)
IF (Immunofluorescence) (Immunofluorescence of Caveolin-1 (green) in Hela using Caveolin-1 antibody at dilution 1:20, and DAPI(blue))
IHC (Immunohiostchemistry) (Immunohistochemistry of Caveolin-1 in paraffin-embedded Human breast cancer tissue using Caveolin-1 Rabbit mAb at dilution 1:100)
WB (Western Blot) (Western blot detection of Caveolin-1 in Hela cell lysates using Caveolin-1 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:20kDa.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
Application Data (Human peripheral blood lymphocytes are stained with APC Anti-Human CD19 Antibody and Fluor Violet 450 Anti-Human CD40 Antibody (Left). Lymphocytes are stained with APC Anti-Human CD19 Antibody and Fluor Violet 450 Mouse IgG1, kappa Isotype Control (Right).)
ICC (Immunocytochemistry) (ICC staining PKR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKR antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PKR on different lysates using anti-PKR antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)
Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nestin antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of A431 cells with Involucrin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Involucrin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Involucrin on human skin tissue lysate using Involucrin antibody at 1/500 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast tissue. 1.alpha Lactalbumin Monoclonal Antibody(9E9)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.alpha Lactalbumin Monoclonal Antibody(9E9) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of 1) Human Milk. 2) Milk. diluted at 1:3000.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDMX Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues. showing nuclear localization with DAB staining using MDMX Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using MDMX Monoclonal Antibody against HeLa (1). A549 (2) and A431 (3) cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry of eIF4EBP1 in paraffin-embedded Human breast cancer tissue using eIF4EBP1 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of eIF4EBP1 in Jurkat,C6,CHO-K1 cell lysates using eIF4EBP1 Rabbit mAb(1:1000 diluted).Predicted band size:13kDa.Observed band size:15-20kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Tissue Factor in paraffin-embedded Human lung cancer tissue using Tissue Factor Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Tissue Factor in Hela,A549,MCF-7,U251,HL-60 cell lysates using Tissue Factor Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:45kDa.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of A549 cells using Catenin-? Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using Catenin-? Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human Placenta tissues with AEC staining using Catenin-? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using Catenin-? Monoclonal Antibody against CTNNB1-hIgGFc transfected HEK293 cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis human colon cancer Caco-2 cells analyzed by flow cytometry using isotype control antibody (left) or Hsp90 beta mAb (K3701) (right).)
WB (Western Blot) (Western blot analysis: Lane 1: MW marker, Lane 2: HeL a, Lane 3: PC-12, Lane 4: CHO-K1, Lane 5: HSP90 Native Protein, Lane 6: HSP90 alpha Recombinant Protein (negative control), probed with HSP90 beta mAb (K3701))
WB (Western Blot) (Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.)
IF (Immunofluorescence) (Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.)
Application Data (Published customer image: Mouse anti Human aggrecan antibody, clone 7E1 used for the detection of aggrecan expressing cells in murine micromass cultures by immunocytochemistry.Image caption: Regulation of Extracellular matrix (ECM) composition by Bone morphogenetic protein (BMP) family growth factors during chondrogenesis. Mouse micromass cultures without treatment (A-E), Gdf-5 treatment (F-J), and Bmp-7 treatment (K-O); Alcian blue stain (A-K) showed cartilage nodules, Immunohistochemical stain for type 1 collagen (A, F, K), type II collagen (C, H, M), aggrecan (D, I, N), and fibronectin (E, J, O). Statistical analysis for histochemical and immunohistochemical data, (P) = percentage of positive area for Alcian blue, type I collagen, type II collagen, aggrecan, and fibronectin stains in micromasses with different treatments; mean values are shown with Standard deviations (SD) (n = three independent experiments), One-way Analysis of variance (ANOVA). ***P )
WB (Western Blot) (Western Blot: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohistochemistry) (Anti-APOA1 / Apolipoprotein A 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohiostchemistry) (EED mAb tested by Immunohistochemistry . EED detection by Immunohistochemistry. The analysis was performed using human tonsil paraffin section and the EED mAb.)
ChIP (Chromatin Immunoprecipitation) (EED antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from a human B cell lymphoma cell line (4.5 million cells) and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 32 million sequence tags were mapped to identify EED binding sites. The image on the left shows EED binding across a 1.6 million bp region on chromosome 6. The image on the right shows EED binding at the STX16 start site.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BUB1B protein in paraffin embedded Adenocarcinoma of Human colon tissue using BUB1B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BUB1B protein (Right) or empty vector (Left) detected with BUB1B antibody)
WB (Western Blot) (Detection of human ARID3A by Western blot.Samples: Whole cell lysate (25 ug) from HEK293 cells. [Lot No. ARIDE9C11-2]Predicted molecular weight: 62 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ARID3A monoclonal antibody.)
WB (Western Blot) (Detection of NEIL2 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2626C2a-1]Predicted molecular weight: 36 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-NEIL2 monoclonal antibody.)
WB (Western Blot) (Western blot analysis of Cytokeratin 8 expression in HaCaT cell lysate (AAA47058).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT8)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody(AAA47058)KRT8 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT8 Antibody (AAA47058)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3-1B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: Citrulline-BSA (0.5 ug). Lane 5: Citrulline-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
IF (Immunofluorescence) (ICC/IF analysis of MAT2A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAT2A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry) (Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Ramos cell lysate Lane 3. : 293T cell lysate Lane 4. : Jurkat cell lysate Lane 5. : NIH-3T3 cell lysate)
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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