Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Immunodetection of htPAPP-A in sandwich immunoassay.AAA57741 is biotinylated.is labeled with stable EU^3+ chelate.Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin coated plates.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD88:Endotoxin Low followed by Rabbit F(ab')2 anti Mouse IgG:FITC)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD88)
Application Data (Staining of paraffin embedded human lung with Mouse anti Human CD88)
IF (Immunofluorescence) (ICC/IF analysis of KRT23 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT23 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Application Data (Fig. 1- Cell surface staining of PBMC stimulted 5hrs with PMA+Ionomycin. Lymphocytes were gated. Green represents IgG1 Isotype control , Red represents Anti-CD69 Clone FN61 (AAA78484). 0.5 ug antibody was used. PE conjugated Goat anti-mouse Ig was used as secondary antibody.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells using DLK1 Monoclonal Antibody (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of U251 cells using DLK1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using DLK1 Monoclonal Antibody against human DLK1 (AA: 174-349) recombinant protein.)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using ER? Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded mammary cancer tissues with DAB staining using ER? Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ER? Monoclonal Antibody against MCF-7 (1). T47D (2) and SKBR3 (3) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of LOVO cells using I-FABP Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using I-FABP Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human Small Intestine tissues with AEC staining using I-FABP Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using I-FABP Monoclonal Antibody against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells using KLHL11 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of 3T3-L1 cells using KLHL11 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
WB (Western Blot) (Western Blot analysis using KLHL11 Monoclonal Antibody against HeLa (1) and MCF-7 (2) cell lysate.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using IRF3 Mouse Monoclonal antibody diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Spleen Tissue using IRF3 Mouse Monoclonal antibody diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)MCF7 Cell. 2) Mouse Heart. 3) Rat Heart Tissue Lysate using IRF3Mouse Monoclonal mAb diluted at 1:2.000.)
Application Data ((0.0003ug/ml) staining in HeLa(A), (0.0001ug/ml) staining in MCF7(B) and (0.0003ug/ml) staining in HepG2(C) cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 ug of HeLa nuclear extract and NCAPH2 antibody at a 1 ug/ml dilution.)
IF (Immunofluorescence) (NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.)
WB (Western Blot) (Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.)
Application Data (Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence)
Application Data (c-myc tagged protein detected with Mouse anti c-myc:Alk Phos)
WB (Western Blot) (Western blot analysis of Amyloid beta A4 expression in mouse brain lysate (AAA46868).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APP)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain, using Amyloid beta A4 Antibody (AAA46868)APP was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APP Antibody (AAA46868)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD40 protein, mFc-His tagged protein PME100015 can bind Rabbit anti-CD40 monoclonal antibody ( clone: DM101) in a linear range of 0.64-80 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD40 protein, mFc-His tagged protein PME100015 can bind Rabbit anti-CD40 monoclonal antibody ( clone: DM101) in a linear range of 0.64-80 ng/ml.)
IF (Immunofluorescence) (Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green).)
WB (Western Blot) (Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IF (Immunofluorescence) (ICC/IF analysis of Visfatin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Visfatin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate (30ug) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : SW480 cell lysate (40ug))
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SNAPC1 mAb. [Lot No.3025C1a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-SNAPC1 monoclonal antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ENPEP protein in paraffin embedded Carcinoma of Human kidney tissue using ENPEP antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ENPEP protein (Right) or empty vector (Left) detected with ENPEP antibody)
Purified Mouse anti-Horse SAA (Clone 4F6) - Monoclonal
Gene Names
SAA2; SAA; SAA1
Reactivity
Horse
Applications
ELISA
Pricing
WB (Western Blot) (Western blot: The image below shows that anti-human IL-17A antibody 2F3 reacts with human 293 cell expressed human IL-17A antigen (10ng, 8ng) in Western Blot. The blot was stained with HRP substrate 4-chlorol-1-Naphthol.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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