Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IHC (Immunohiostchemistry) (Immunohistochemistry of Clathrin heavy chain in paraffin-embedded Human colon cancer tissue using Clathrin heavy chain Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of Clathrin heavy chain in Hela,A549,HL-60,U251,U87-MG cell lysates using Clathrin heavy chain Rabbit mAb(1:1000 diluted).Predicted band size:192kDa.Observed band size:192kDa.)

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Clathrin Heavy Chain, Monoclonal Antibody (Cat# AAA314517)

Full Name

Clathrin Heavy Chain Rabbit mAb

Gene Names

CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

Application Data

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Digoxigenin, Monoclonal Antibody (Cat# AAA47771)

Full Name

Digoxigenin Monoclonal Antibody

Applications

Immunohistochemistry, Immunoblot

Pricing

$650/0.1 mg $1,260/2x0.1 mg $1,845/3x0.1 mg $2,390/4x0.1 mg $2,735/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of MAPK8 protein in paraffin embedded Carcinoma of Human prostate tissue using MAPK8 antibody)

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MAPK8, Monoclonal Antibody (Cat# AAA108201)

Full Name

MAPK8 antibody

Applications

Western Blot, Immunohistochemistry

Purity

MAPK8 antibody was purified by affinity chromatography.

Pricing

$1,415/0.1 mL $2,755/2x0.1 mL $4,020/3x0.1 mL $5,220/4x0.1 mL $6,135/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Midkine Antibody (AAA47083)MDK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDK Antibody (AAA47083)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Western blot analysis of Midkine expression in Midkine Recombinant protein (AAA47083).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MDK)

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Midkine, Monoclonal Antibody (Cat# AAA47083)

Full Name

Anti-Midkine Rabbit Monoclonal Antibody

Gene Names

MDK; MK; ARAP; NEGF2

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human Dectin-1:FITC)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human Dectin-1:Alexa Fluor 488 (AAA49749A488))

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human Dectin-1:RPE)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human Dectin-1:Alexa Fluor ?647 (AAA49749A647))

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DECTIN-1, Monoclonal Antibody (Cat# AAA49749)

Full Name

MOUSE ANTI HUMAN DECTIN-1

Gene Names

CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12

Applications

Flow Cytometry

Pricing

$575/0.2 mg $1,110/2x0.2 mg $1,620/3x0.2 mg $2,105/4x0.2 mg $2,360/5x0.2 mg

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab')2 anti Mouse IgG:FITC)

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CD39, Monoclonal Antibody (Cat# AAA49802)

Full Name

MOUSE ANTI HUMAN CD39

Gene Names

ENTPD1; CD39; SPG64; ATPDase; NTPDase-1

Applications

Immunofluorescence, Flow Cytometry

Pricing

$415/0.1 mg $810/2x0.1 mg $1,175/3x0.1 mg $1,525/4x0.1 mg $1,655/5x0.1 mg

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Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P:Alexa Fluor 647)

Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P)

Application Data (Staining of thrombin activated dog platelets with Mouse anti Human CD62P followed by goat anti mouse IgG:FITC)

Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P:RPE)

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CD62P, Monoclonal Antibody (Cat# AAA49677)

Full Name

MOUSE ANTI HUMAN CD62P

Gene Names

Selp; PSELECT

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

$245/0.025 mg $480/2x0.025 mg $700/3x0.025 mg $895/5x0.025 mg $910/4x0.025 mg

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IHC (Immunohiostchemistry) (Anti-RBP4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry) (Anti-RBP4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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RBP / RBP4, Monoclonal Antibody (Cat# AAA51827)

Full Name

Mouse Monoclonal [clone AT2B4] (IgG1,k) to Human RBP / RBP4

Gene Names

RBP4; RDCCAS

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Western blot of anti-AXL monoclonal antibody against extracellular domain of human AXL(aa19-444).)

WB (Western Blot) (Confocal immunofluorescence of methanol-fixed HEK293 cells transfected with AXL-hIgGFc using anti-AXL monoclonal antibody(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.)

IHC (Immunohiostchemistry) (Anti-AXL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

IHC (Immunohistochemistry) (Anti-AXL antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

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AXL, Monoclonal Antibody (Cat# AAA51852)

Full Name

Mouse Monoclonal [clone 7E10] (IgG1) to Human AXL

Gene Names

AXL; ARK; UFO; JTK11; Tyro7

Reactivity

Human

Applications

Immunohistochemistry

Purity

Ascites

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Histone H2Av antibody (mAb) tested by Western blot. Detection of Histone H2Av by Western blot. The analysis was performed using Schneider’s Drosophila L2 nuclear extract (20ug)and the Histone H2Av antibody at a dilution of 1ug/ml.)

ChIP (Chromatin Immunoprecipitation) (Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

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Histone H2Av, Monoclonal Antibody (Cat# AAA60082)

Full Name

Histone H2Av antibody (mAb)(Clone 10E9.D1)

Reactivity

Drosophila

Applications

Western Blot, Chromatin immunoprecipitation

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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Application Data (1x106 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(AAA178662) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L))

IP (Immunoprecipitation) (IP Result of 293F cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody)

IF (Immunofluorescence) (Immunofluorescent analysis of 293F cells transfected with the DYKDDDDK-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.)

WB (Western Blot) (Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.)

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GFP, Monoclonal Antibody (Cat# AAA178662)

Full Name

GFP Monoclonal Antibody

Reactivity

All

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence, Western Blot

Purity

Protein A/G Purified

Pricing

$155/0.025 mL $220/0.1 mL $430/2x0.1 mL $630/3x0.1 mL $815/4x0.1 mL $980/5x0.1 mL

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Application Data (Cytokeratin 19 Antibody on Colon- 40x)

Application Data (Cytokeratin 19 Antibody (P1008) on Colon- 20x)

Application Data (Cytokeratin 19 Antibody (P1008) on Colon- 10x)

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Cytokeratin 19, Monoclonal Antibody (Cat# AAA312717)

Full Name

Cytokeratin 19 Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human

Applications

Immunohistochemistry

Pricing

$285/0.1 mL $705/1 mL $1,375/2x1 mL $2,005/3x1 mL $2,605/4x1 mL $2,955/5x1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of IRF7 in paraffin-embedded Human breast cancer tissue using IRF7 Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of IRF7 in Rat Brain lysates using IRF7 Rabbit mAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:54kDa.)

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IRF7, Monoclonal Antibody (Cat# AAA314487)

Full Name

IRF7 Rabbit mAb

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysateAll lanes: HMGB1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 25 kDaObserved band size: 25 kDa)

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HMGB1, Monoclonal Recombinant Antibody (Cat# AAA243907)

Full Name

HMGB1 Antibody

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded lung cancer (left) and colon tumour tissues (right) with DAB staining using IMP-3 Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis using IMP-3 Monoclonal Antibody against Jurkat (1). K562 (2) and NTERA-2 (3) cell lysate.)

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IMP-3, Monoclonal Antibody (Cat# AAA320116)

Full Name

IMP-3 Monoclonal Antibody

Gene Names

IGF2BP3; CT98; IMP3; KOC1; IMP-3; VICKZ3

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B). showing cytoplasmic localization with AEC staining (A) and DAB staining(B) using TRIM5? Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis using TRIM5? Monoclonal Antibody against human breast carcinoma tissue lysate.)

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TRIM5alpha, Monoclonal Antibody (Cat# AAA320247)

Full Name

TRIM5alpha Monoclonal Antibody

Gene Names

TRIM5; RNF88; TRIM5alpha

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$310/0.05 mL $430/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Integrin beta 1 expression in A431 cell lysate (AAA46990).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGB1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using Integrin beta 1 Antibody(AAA46990)ITGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGB1 Antibody (AAA46990)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Integrin beta 1, Monoclonal Antibody (Cat# AAA46990)

Full Name

Anti-Integrin beta 1 Rabbit Monoclonal Antibody

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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Application Data (SDS-PAGE analysis of GM-1D6 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified GM-1D6 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-1D6 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)

Application Data (Fig.2: Spectral Confocal Microscopy of CHO cells using GM1-D6 Cat.# GM-0601. CHO cells were transiently transfected with an expression vector encoding FPRL1. Binding of GM-1D6 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)

Application Data (Fig.1: FACS analysis of BOSC23 cells using GM- 1D6 Cat.# GM0601. BOSC23 cells were transiently transfected with an expression vector encoding either FPRL1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM- 1D6 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FPRL1 transfected cells.)

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FPRL1, Monoclonal Antibody (Cat# AAA71466)

Full Name

anti-human formyl peptide-receptor-like-1 (FPRL1) monoclonal antibody

Gene Names

FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II

Reactivity

Human

Applications

Flow Cytometry

Purity

Protein G

Pricing

$630/0.1 mg $1,220/2x0.1 mg $1,780/3x0.1 mg $2,315/4x0.1 mg

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ICC (Immunocytochemistry) (Anti-Cyclin A antibody (monoclonal), AAA44885, ICCICC: SMMC-7721 Cell)

ICC (Immunocytochemistry) (Anti-Cyclin A antibody (monoclonal), AAA44885, ICCICC: A549 Cell)

WB (Western Blot) (Anti-Cyclin A antibody (monoclonal), AAA44885, Western blottingLane 1: HELA Cell LysateLane 2: JURKAT Cell Lysate)

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Cyclin A, Monoclonal Antibody (Cat# AAA44885)

Full Name

Anti-Cyclin A antibody (monoclonal)

Gene Names

CCNA2; CCN1; CCNA

Reactivity

Human, Mouse, Bovine

Applications

Immunocytochemistry, Western Blot

Purity

Ascites

Pricing

$485/0.1 mg $945/2x0.1 mg $1,385/3x0.1 mg $1,795/4x0.1 mg $2,005/5x0.1 mg

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LSM8 mAb. [Lot No.2527C6a-1])

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-LSM8 monoclonal antibody.)

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LSM8, Monoclonal Antibody (Cat# AAA36393)

Full Name

Mouse monoclonal antibody Anti-Human LSM8

Gene Names

LSM8; LSM8; NAA38

Reactivity

Human

Applications

Immunocytochemistry, Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

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Application Data (Staining of rat spleen cells with Mouse anti Rat CD45:Alexa Fluor 488)

Application Data (Staining of rat spleen cells with Mouse anti Rat CD45: RPE)

Application Data (Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD45)

Application Data (Staining of rat spleen cells with Mouse anti Rat CD45:FITC)

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CD45, Monoclonal Antibody (Cat# AAA49505)

Full Name

MOUSE ANTI RAT CD45:RPE

Gene Names

Ptprc; Lca; RT7; CD45; L-CA; T200

Applications

Flow Cytometry

Pricing

$630/100 Tests $1,220/2x100 Tests $1,780/3x100 Tests $2,315/4x100 Tests $2,605/5x100 Tests

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WB (Western Blot) (Western blot of CD38 mAb against CD38-hIgGFc transfected HEK293 cell lysate.)

IHC (Immunohiostchemistry) (Anti-CD38 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

IHC (Immunohistochemistry) (Anti-CD38 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

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CD38, Monoclonal Antibody (Cat# AAA51941)

Full Name

Mouse Monoclonal [clone 6E12D] (IgG1) to Human CD38

Gene Names

CD38; T10; ADPRC 1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Ascites

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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Application Data (Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD31:FITC)

Application Data (Published customer image:CAM expression in M. suis infected endothelial cells. The expression profiles of adhesion molecules on endothelial cells were analyzed using a FACSCanto II cell sorting system. A. Dashed lines represent the isotype control; solid lines represent cells incubated with the negative control preparation; grey histograms represent PEDSV.15 cells incubated with M. suis (1 x 104 cells/mL). B. Percent increase in expression ICAM-1 (CD54), PECAM-1 (CD31), E (CD62E)- and P (CD62P)-selectin in ECs infected with M. suis.From: Sokoli, A. et al. œMycoplasma Suis Infection Results Endothelial Cell Damage and Activation: New Insight into the Cell Tropism and Pathogenicity of Hemotrophic Mycoplasma. Veterinary Research 44, no. 1 (2013): 6.)

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CD31, Monoclonal Antibody (Cat# AAA49603)

Full Name

MOUSE ANTI PIG CD31:RPE

Gene Names

PECAM1; CD31; PECAM-1

Applications

Flow Cytometry

Pricing

$305/25 Tests $585/2x25 Tests $850/3x25 Tests $1,105/4x25 Tests $1,140/5x25 Tests

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IP (Immunoprecipitation)

IF (Immunofluorescence)

WB (Western Blot)

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GFP-Tag, Monoclonal Antibody (Cat# AAA62302)

Full Name

GFP-Tag (7G9) Mouse mAb

Reactivity

All

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Pricing

$285/0.05 mL $355/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,795/5x0.2 mL

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Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-HHEX monoclonal antibody.)

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HHEX, Monoclonal Antibody (Cat# AAA36326)

Full Name

Mouse monoclonal antibody Anti-Human HHEX

Gene Names

HHEX; HEX; PRH; HMPH; PRHX; HOX11L-PEN

Reactivity

Human

Applications

ELISA

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

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Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-XBP1 monoclonal antibody.)

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XBP1, Monoclonal Antibody (Cat# AAA36350)

Full Name

Mouse monoclonal antibody Anti-Human XBP1

Gene Names

XBP1; XBP2; TREB5; XBP-1; TREB-5

Reactivity

Human

Applications

Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using JAK1 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using JAK1 Mouse mAb diluted at 1:200.)

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JAK1, Monoclonal Antibody (Cat# AAA243630)

Full Name

JAK1 Monoclonal Antibody

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (0.5ug HA fusion protein + Primary antibody dilution at 1:10000)

IF (Immunofluorescence) (IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA))

IP (Immunoprecipitation) (IP antibody use: 5ug HA Mouse IgG1 per ml Lysate, WB 1:5000Lane 1: untransfected 293 cell lysateLane 2: transfected 293 cell lysate with HA-tag fusion proteinLane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)Lane 6: IP (transfected 293 + Protein G)Lane 7: Recombinant protein (E.coli))

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HA-Tag, Monoclonal Antibody (Cat# AAA243713)

Full Name

HA-Tag Monoclonal Antibody

Reactivity

General

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells, using Cytokeratin 17 Antibody .)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil, using Cytokeratin 17 Antibody.)

WB (Western Blot) (Western blot analysis of Cytokeratin 17 expression in HACAT cell lysate.)

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Cytokeratin 17, Monoclonal Recombinant Antibody (Cat# AAA315213)

Full Name

Cytokeratin 17 Rabbit mAb

Gene Names

KRT17; PC; K17; PC2; PCHC1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Human placenta tissue was stained with Anti-Annexin A1 (PT1761) Antibody)

IHC (Immunohistochemistry) (Human esophagus tissue was stained with Anti-Annexin A1 (PT1761) Antibody)

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Annexin A1, Monoclonal Antibody (Cat# AAA320581)

Full Name

Annexin A1 (PT1761) Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Protein G

Pricing

$215/0.02 mL $290/0.05 mL $350/0.1 mL $575/0.2 mL $1,110/2x0.2 mL $1,620/3x0.2 mL $2,105/4x0.2 mL $2,325/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.)

Application Data (Ge. Lu. et al. "PRMT5 promotes epithelial?mesenchymal transition via EGFR???catenin axis in pancreatic cancer cells." Journal of cellular and molecular medicine 24.2 (2020): 1969-1979.)

WB (Western Blot) (Western blot analysis of PC3 Cell Lysate using Akt Mouse mAb diluted at 1:2000)

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Akt, Monoclonal Antibody (Cat# AAA320511)

Full Name

Akt (PTR2314) Mouse Monoclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Protein G

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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Application Data (Wei. Yuzhen. et al. "Inhibition of microRNA?155 ameliorates cardiac fibrosis in the process of angiotensin II?induced cardiac remodeling." Molecular medicine reports 16.5 (2017): 7287-7296.)

WB (Western Blot) (Western blot analysis of 1) Hela. 2) 3T3. 3) Rat Brain using ?-SMA Monoclonal Antibody.)

Application Data (The picture was kindly provided by our customer. Primary antibody was diluted at 1:5000. Loading control antibody was diluted at 1:20000)

Application Data (The picture was kindly provided by our customer)

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alpha-SMA, Monoclonal Antibody (Cat# AAA320483)

Full Name

alpha-SMA Monoclonal Antibody (1E12)

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

$275/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

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O-GlcNAc, Monoclonal Antibody (Cat# AAA103983)

Full Name

O-GlcNAc Antibody, Clone 9H6: HRP

Reactivity

Species Independent

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

PEG Purified

Pricing

$665/0.1 mg $1,295/2x0.1 mg $1,890/3x0.1 mg $2,450/4x0.1 mg $2,685/5x0.1 mg

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Application Data

Application Data

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IL-6, Monoclonal Antibody (Cat# AAA60744)

Full Name

Anti-IL-6 Antibody

Gene Names

IL6; HGF; HSF; BSF2; IL-6; IFNB2

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Figure 5 Pseudovirus neutralization assayAntibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibodies, AAA40988 (A10) and SD9855 (E10). Luciferase reporter virus-like particles and 293T-hsACE2 cells were used for the assay. After 72 hrs incubation infectivity was measured by luciferase expression using Promega Renilla-Glo Luciferase Assay System. Infectivity was measured as RLUs and no sdAb control was set to 100% infectivity. Neutralization activity of AAA40988 (A10) and SD9855 (E10) was measured over a serial dilution series to determine the half-maximal inhibitory concentration (IC50). Both AAA40988 (A10) and SD9855 (E10) exhibited a dose dependent neutralizing effect on both alpha pseudoviruses, and the combination of the two showed a significantly synergistic effect.)

Application Data (Figure 4 Pseudovirus neutralization assayAntibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibodies, AAA40988 (A10) and SD9855 (E10). Luciferase reporter virus-like particles and 293T-hsACE2 cells were used for the assay. After 72 hrs incubation infectivity was measured by luciferase expression using Promega Renilla-Glo Luciferase Assay System. Infectivity was measured as RLUs and no sdAb control was set to 100% infectivity. Neutralization activity of AAA40988 (A10) and SD9855 (E10) was measured over a serial dilution series to determine the half-maximal inhibitory concentration (IC50). Both AAA40988 (A10) and SD9855 (E10) exhibited a dose dependent neutralizing effect on both wild-type pseudoviruses, and the combination of the two showed a significantly synergistic effect.)

ELISA (Figure 3 ACE2-RBD binding inhibitory ELISA Antibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibodies, AAA40988 (A10) and SD9855 (E10). ACE2-RBD binding inhibitory ELISA was performed using RBD protein of SARS-CoV-2 variants (wild-type and alpha) as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) RBD antibody (AAA40988 and SD9855) as the capture antibody, followed by incubation with 20 ng/mL human ACE2-Fc. Bound h-ACE2-Fc was detected with a goat anti-human IgG-HRP conjugate (1:20,000 dilution) using the TMB chromogenic substrate system. Both AAA40988 (A10) and SD9855 (E10) exhibited a dose dependent inhibitory effect on ACE2 binding to RBDs of wild-type and alpha strains, and the combination of the two (A10+E10) showed a significantly synergistic effect.)

ELISA (Figure 2 ELISA Validation with RBDs of SARS-CoV-2 VariantsAntibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibody, AAA40988. A direct ELISA was performed using RBD protein of SARS-CoV-2 variants (wild-type, alpha, beta, gamma and Delta) as coating antigens at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) RBD antibody (AAA40988) as the capture antibody, followed by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. AAA40988 binds to RBDs of wild-type and alpha but not to the beta, gamma and Delta variants.)

Application Data (Figure 1 Neutralization Assay of SARS-CoV-2 Pseudovirus by SARS-CoV-2 Spike Antibody Neutralization antibody: Anti-SARS-CoV-2 Spike S1 antibody, AAA40988, dilution: 0.1 - 200 ng/mL.Pseudovirus: SARS-CoV-2 Spike Pseudovirus, 95-200. Cells: ACE2-overexpressing 293T cell. Diluted antibody was incubated with Spike pseudovirus at 37 degree C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 degree C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.)

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SARS-CoV-2 (COVID-19) Spike Neutralization, Monoclonal Recombinant Antibody (Cat# AAA40988)

Full Name

SARS-CoV-2 (COVID-19) Spike Neutralization Single Domain Antibody [A10]

Reactivity

Virus

Applications

Neutralization Assay

Purity

SARS-CoV-2 (COVID-19) Spike Neutralization Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.

Pricing

$630/0.1 mg $1,220/2x0.1 mg $1,780/3x0.1 mg $2,315/4x0.1 mg $2,590/5x0.1 mg

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WB (Western Blot) (Detection of human TAX1BP1 by Western blot.Samples: Whole cell lysate (50 ug) from HeLa cells. [Lot No. 3098C2a-1]Predicted molecular weight: 90 kDa)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-TAX1BP1 monoclonal antibody.)

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TAX1BP1, Monoclonal Antibody (Cat# AAA36426)

Full Name

Mouse monoclonal antibody Anti-Human TAX1BP1

Gene Names

TAX1BP1; T6BP; TXBP151; CALCOCO3

Reactivity

Human

Applications

Western Blot, Dot Blot

Pricing

$590/0.1 mg $1,140/2x0.1 mg $1,665/3x0.1 mg $2,160/4x0.1 mg $2,410/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)

IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western BlotPositive WB detected in: U-251 whole cell lysateAll lanes: Mutant p53 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38, 39, 40, 34, 35, 30, 24, 25 kDaObserved band size: 53 kDa)

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TP53, Monoclonal Recombinant Antibody (Cat# AAA243961)

Full Name

TP53 Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

ELISA (It is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.)

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COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody Pair Kit (Cat# AAA244025)

Full Name

SARS-CoV-2 N Antibody Pair 2

Applications

ELISA

Pricing

$1,010/1 Pair (Sufficient for 5x96 well plates) $1,970/2x1 Pair (Sufficient for 5x96 well plates) $4,680/5x1 Pair (Sufficient for 25x96 well plates)

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma Tissue using SGK1 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using SGK1 Mouse mAb diluted at 1:200.)

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SGK1, Monoclonal Antibody (Cat# AAA243632)

Full Name

SGK1 Monoclonal Antibody

Gene Names

SGK1; SGK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1gamma Mouse mAb diluted at 1:1000.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1gamma Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1gamma Mouse mAb diluted at 1:200.)

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CBX3, Monoclonal Antibody (Cat# AAA243650)

Full Name

CBX3 Monoclonal Antibody

Gene Names

CBX3; HECH; HP1-GAMMA; HP1Hs-gamma

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Muscle Actin Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muscle Tissue using Muscle Actin Mouse mAb diluted at 1:200.)

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Muscle actin, Monoclonal Antibody (Cat# AAA309498)

Full Name

Muscle actin Mouse Monoclonal Antibody(2H3)

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Beclin-1 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.)

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Beclin-1, Monoclonal Antibody (Cat# AAA309511)

Full Name

Beclin-1 Mouse Monoclonal Antibody(5C5)

Gene Names

BECN1; beclin-1

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using JAK2 Mouse mAb diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using JAK2 Mouse mAb diluted at 1:200)

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JAK2, Monoclonal Antibody (Cat# AAA309536)

Full Name

JAK2 Mouse Monoclonal Antibody(9B4)

Gene Names

jak2b; jak2; JAK2bCA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.